Click here to switch to the map view.

The map label for this gene is sqdB [C]

Identifier: 123969406

GI number: 123969406

Start: 1608540

End: 1609733

Strand: Direct

Name: sqdB [C]

Synonym: A9601_18741

Alternate gene names: 123969406

Gene position: 1608540-1609733 (Clockwise)

Preceding gene: 123969405

Following gene: 123969407

Centisome position: 96.33

GC content: 35.43

Gene sequence:

>1194_bases
GTGAAAGTTATTGTTCTAGGTGGAGATGGTTTTTGCGGTTGGCCTTGTGCGGTGAATTTAGCAGAGCAAAATCATGATGT
AATTATTGTCGACAATTTAAGTCGTAGAAAAATTGATATTGATCTAGAGGTAGAATCTTTAACTCCAATTTCTTCCATAA
CAGAACGACTTTCTGCATGGGAAGAGACTGGAGGCAAGCCTATGAGATTTCTTAACATGGATATCTCTAAGCAATATCAA
AAATTACTCAATTTGCTCATTGATGAAAAACCAGATTCCGTGATCCATTTTGCAGAACAAAGAGCAGCACCATACTCGAT
GAAATCGAGTTTTACCAAAAGATATACAGTAGATAATAATGTTAATGGCACCCACAACTTGCTTGCTGCGATAGTAGAGA
GTAATTTAGATATTCATGTTGTTCATTTAGGAACAATGGGAGTCTACGGATATGGATCACATAGAGGTGCAACAATTCCA
GAAGGTTATCTAAAAGTTGAAGTTCCACAACCTGATGGAAGCCGCTTTGAAGAAGAAATATTACACCCTGCAAGCCCAGG
TAGTGTCTACCACATGACTAAAACTTTAGATCAATTATTATTTCTTTACTACAACAAAAATGATCTTGTAAGGATCACTG
ATCTACACCAAGGCATTGTTTGGGGAACAAATACAGAAGCAACTTTAAAAGATCCTAGATTGACAAACAGATTTGACTAT
GACGGAGATTATGGAACTGTTTTAAACAGATTTCTAATGCAAGCTGCAATTGGATATCCATTAAGTGTTCATGGGACAGG
AGGGCAAACAAGAGCATTTATACATATAAAAGACTCTGTCAAATGCGTACAACTTGCTCTTGAAAATCCTCCAAAATCTG
GAGAAAGAGTCAAAATCTTTAATCAAATGACTGAGAGTCATCAAGTTGGAGAACTAGCTAAAAAAGTTGCTTCTCTAACT
GGAGCTGAAATCAATTATTTACCAAATCCAAGGAATGAAGCAGTAGAAAATGATCTAATTGTTGATAATAAATGCTTTAT
AGAATTAGGTTTAAACCCAACTACTCTTGATAATGGCTTATTAGAAGAAGTTGTTGAAGTTGCTAAAAAATACTCCAATA
GATGTGATCTTAATCGCATACCTTGTGTTTCATCCTGGACAAAAAAACAAGCTGAGGCTATTAAGACTAATTAA

Upstream 100 bases:

>100_bases
AAGAAGATTTGAAACTATTTACAATGTTTTTTCGCAAAGTGGAAGCACGCTGTAGTAATTTAATAAATATATTATGAATC
TTTTAATTCTGGAGTTCTGA

Downstream 100 bases:

>100_bases
AATTTTGAAATAGTTATCTTAAGAAAGTGAAAATTGCATTGTTTACTGAAACTTTTTTACCTAAAGTTGACGGCATAGTC
ACAAGACTGACTAAAACAAT

Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; DTDP-Glucose 4-6-Dehydratase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ
KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT
GAEINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN

Sequences:

>Translated_397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ
KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT
GAEINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN
>Mature_397_residues
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAWEETGGKPMRFLNMDISKQYQ
KLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNNVNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIFNQMTESHQVGELAKKVASLT
GAEINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGLLEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=150, Percent_Identity=34.6666666666667, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44359; Mature: 44359

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
EETGGKPMRFLNMDISKQYQKLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN
HHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC
VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEHH
NQMTESHQVGELAKKVASLTGAEINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVIVLGGDGFCGWPCAVNLAEQNHDVIIVDNLSRRKIDIDLEVESLTPISSITERLSAW
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
EETGGKPMRFLNMDISKQYQKLLNLLIDEKPDSVIHFAEQRAAPYSMKSSFTKRYTVDNN
HHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHCCCEEEEECCC
VNGTHNLLAAIVESNLDIHVVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCCHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDLVRITDLHQGIVWGTNTEATLKDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCEEEEEECCCCEEECCCCCCEECCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLSVHGTGGQTRAFIHIKDSVKCVQLALENPPKSGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEHH
NQMTESHQVGELAKKVASLTGAEINYLPNPRNEAVENDLIVDNKCFIELGLNPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVEVAKKYSNRCDLNRIPCVSSWTKKQAEAIKTN
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA