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The map label for this gene is thiG [H]

Identifier: 123969405

GI number: 123969405

Start: 1607574

End: 1608368

Strand: Direct

Name: thiG [H]

Synonym: A9601_18731

Alternate gene names: 123969405

Gene position: 1607574-1608368 (Clockwise)

Preceding gene: 123969404

Following gene: 123969406

Centisome position: 96.27

GC content: 35.85

Gene sequence:

>795_bases
ATGGAAAATTATTCTTCTTTACTAATTGGTGGAAAACAATTTTCCAGTAGATTAATGGTTGGTACTGGCAAATACAAATC
TACTCAAGATATGGTGGCAAGTTTGTCAAATTCTGAAACGGAAATTATAACCGTCGCTGTTAGAAGAATTAAAAATGATC
AGACCGGAGAAAATTTACTCGAAAAGATCAACTGGGAAAAATACTGGATGCTTCCTAATACAGCTGGTTGTGTTAATTCC
GATGAGGCAGTCAGAATAGCAATTTTAGGTAGAGAACTTGCAAAATTATCTGGTCAAGAAGAAAATAATTTTGTGAAGTT
AGAAGTTATTCCTGACAAAAAGTATTTGCTACCAGATCCAATAGAAACTCTTAAAGCAGCCGAAATTTTAATTAAAAAAG
GTTTCGCTGTACTACCTTATATCAATGCAGATCCCATTCTTGCAAAAAGACTAGAAGAAATAGGTTGTGCAACTGTAATG
CCATTGGGCTCTCCCATAGGCTCCGGGCAAGGTTTGTTAAATTTATCAAATATAAGGATTATTATTGAGAATTCAAAAGT
GCCAGTAATAATTGACGCAGGAATTGGGGTACCTAGTGAAGCTTCTCAAGCTATGGAAATTGGAGCTGATGGTGTTTTAA
TCAATAGTGCAATAGCACAAGCTGCAAATCCTCCTCTAATGGCTCAAGCGATAAATTATAGTGTGAAAGCTGGCAGGCAA
GCTTTTCTGGCAGGAAGAATTAAAAAACAAGACTTTGCAGTAGCAAGTTCGCCGGAAAAAAATATATCTATCTAA

Upstream 100 bases:

>100_bases
TAGATCCAAAAAACAAAACAGCAATAAAAAAAAGTAAGGAATTAGAAAAAAGAAACAATTATGAACCTTATAAAGGTATT
AAGGATAAGGGATTCTAAAT

Downstream 100 bases:

>100_bases
TTCTCTAAATTGAGAAAAGTTTCAAAAGAAAGTAAAAATTTATTATTTGCTTTTATTTATCCTATTTAAGAAAGAAGATT
TGAAACTATTTACAATGTTT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MENYSSLLIGGKQFSSRLMVGTGKYKSTQDMVASLSNSETEIITVAVRRIKNDQTGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENSKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI

Sequences:

>Translated_264_residues
MENYSSLLIGGKQFSSRLMVGTGKYKSTQDMVASLSNSETEIITVAVRRIKNDQTGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENSKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI
>Mature_264_residues
MENYSSLLIGGKQFSSRLMVGTGKYKSTQDMVASLSNSETEIITVAVRRIKNDQTGENLLEKINWEKYWMLPNTAGCVNS
DEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDPIETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVM
PLGSPIGSGQGLLNLSNIRIIIENSKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
AFLAGRIKKQDFAVASSPEKNISI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=49.2063492063492, Blast_Score=243, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28438; Mature: 28438

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENYSSLLIGGKQFSSRLMVGTGKYKSTQDMVASLSNSETEIITVAVRRIKNDQTGENLL
CCCCCCEEECCHHHCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHH
EKINWEKYWMLPNTAGCVNSDEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVMPLGSPIGSGQGLLNLSNIRI
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECEEEE
IIENSKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
EEECCCCCEEEECCCCCCCCHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCHH
AFLAGRIKKQDFAVASSPEKNISI
HHHHHCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MENYSSLLIGGKQFSSRLMVGTGKYKSTQDMVASLSNSETEIITVAVRRIKNDQTGENLL
CCCCCCEEECCHHHCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHH
EKINWEKYWMLPNTAGCVNSDEAVRIAILGRELAKLSGQEENNFVKLEVIPDKKYLLPDP
HHCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCH
IETLKAAEILIKKGFAVLPYINADPILAKRLEEIGCATVMPLGSPIGSGQGLLNLSNIRI
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECEEEE
IIENSKVPVIIDAGIGVPSEASQAMEIGADGVLINSAIAQAANPPLMAQAINYSVKAGRQ
EEECCCCCEEEECCCCCCCCHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCHH
AFLAGRIKKQDFAVASSPEKNISI
HHHHHCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA