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The map label for this gene is gyrB [H]

Identifier: 123969376

GI number: 123969376

Start: 1573277

End: 1575244

Strand: Reverse

Name: gyrB [H]

Synonym: A9601_18441

Alternate gene names: 123969376

Gene position: 1575244-1573277 (Counterclockwise)

Preceding gene: 123969381

Following gene: 123969375

Centisome position: 94.33

GC content: 34.6

Gene sequence:

>1968_bases
ATGAGTGAGGACAAAAGATCTAATAAAATCTCAAATGATTATGGCGCGGATCAGATTCAGGTTTTAGAGGGGTTAGAACC
AGTTCGAAAACGACCTGGTATGTATATAGGTTCAACAGGGCCTAGGGGATTACATCATTTAGTATATGAGGTAGTTGATA
ACTCAGTTGATGAAGCACTTGCAGGACATTGTGATCACATTGAAATAGTTCTTCGAGCAGATGGTTCTGCTTTTATTTCT
GATAATGGAAGAGGTATTCCAACAGATATTCATCCAAGAACAGGGAAAAGTGCCCTAGAGACTGTACTAACTGTTCTGCA
TGCAGGAGGTAAATTTGGAAGTGGTGGTTATAAAGTCTCAGGGGGTTTGCATGGAGTTGGAATATCTGTAGTTAATGCTC
TAAGCGAATGGGTTAATGTGACTGTTTATAGGGATGGAAGCGAATTTAATCAAAGATTTGAAAAAGGTGTATCAAAGGGT
GAATTGCAAGCTAAAAAGCAGACTGGTAAGCCATTTAAGAAAGGGACAAGTATTTGCTTTAAACCCGACAAAACTATTTT
TTCTGGCGGAATTGAGTTTGAATATGCTCTTCTTTCTTCTAGATTAAGGGAATTAGCTTATCTAAATGGCGGAGTAAAAA
TTGTTTTTAGAGATGAAAGAAATCAATTATCAGATGGTTCCTTTAAAGAAGAAATTTATTTGTATCAAGGAGGTATTAAA
GAATATGTTGAATACATGAATGCTGAAAAAGATTCTATTCATCCTGAAATAATTTATGTTGACTCACAGAAAGAAAATGT
ATATGTAGAAGCAGCTTTACAATGGTGTTCAGATGTATATTCAGATAATATTTTAGGGTTTGCGAATAATATTAGAACTA
TTGATGGAGGGACTCACATTGAAGGGTTAAAAACAGTTCTGACAAGAACTTTCAATAATCTTGCAAAAAAGAGGGGCAAA
AGAAAAGATATTGAAAAAAATTTAGCTGGCGAAAATATTAGAGAGGGTTTGACTGTTGTTTTATCGGTAAAAGTCCCAGA
TCCCGAATTTGAAGGGCAAACAAAAACAAAGTTAGGAAATACTGAAGTACGAGGAATTGTGGATTCTCTCATTGGGGAGG
CTCTTACAAAATATATGGAATTCAATCCTGCAATTTTGGATTTGATCCTTGAAAAAGCAATTCAATCATTTAATGCAGCA
GAAGCTGCGAGAAGGGCTAGAGAATTAGTAAGAAGAAAAAGTGTTCTAGAAAGTTCCACTTTACCTGGCAAATTAGCAGA
TTGTAGTTCTAGAGATCCCTCAGAATCAGAAATTTATATAGTTGAAGGAGATTCAGCTGGAGGTTCTGCAAAACAAGGAC
GAGATAGAAATTTTCAGGCTATTTTGCCTCTCAGGGGAAAAATTCTTAATATTGAAAAAACTGACGATACTAAAATATAT
AAAAATACAGAAATACAGTCATTAATAACAGCTCTAGGATTAGGAATAAAAGGAGAGGAGTTCGACGAAAGCTCTTTGAG
ATATCATAGGGTTGTTATTATGACGGATGCTGATGTTGACGGTGCTCATATAAGAACTTTATTGCTGACATTTTTTTATA
GATACCAAAGAGAACTTGTTGAGAAAGGTTTTATATATATTGCTTGTCCCCCTCTTTATAAAGTTGAAAGAGGTAAAAAT
CATAATTACTGTTATAACGAGAATCAATTAAAGGATACAATTCAAGGTTTTGGAGAAAATGCAAATTATAATATTCAAAG
ATTTAAGGGACTTGGTGAGATGATGCCAAAACAATTATGGGATACAACTATGAATCCCCAAACGAGGATGATGAAAAGGG
TTGAAATCGAAGATGCACTTGAAGCTGACAGAATATTTAATATATTAATGGGAGATAAAGTTGCACCAAGAAGAGAATTT
ATTGAAACCCATAGTGGCAACTTAGATATGGCAACTTTAGACATATGA

Upstream 100 bases:

>100_bases
CTTTTCATTGACTTCGAGCCTTTATCTGATTGCGGTGGTAAGTTTAAATAAAGATCTTTTAAAAATAACATTTTAAAAGT
TGAATATTTTTTTATATTAA

Downstream 100 bases:

>100_bases
TTAATAATGTTCTCAAGAATTTTTGTTTAATTACTTTTGCATTAATTGCTCCAATTACATTACCTGCTGGTGGGATCCAA
AGAAGTTTAAATCAAAATAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSEDKRSNKISNDYGADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLRADGSAFIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSKG
ELQAKKQTGKPFKKGTSICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
EYVEYMNAEKDSIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPAILDLILEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSGNLDMATLDI

Sequences:

>Translated_655_residues
MSEDKRSNKISNDYGADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLRADGSAFIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSKG
ELQAKKQTGKPFKKGTSICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
EYVEYMNAEKDSIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPAILDLILEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKN
HNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSGNLDMATLDI
>Mature_654_residues
SEDKRSNKISNDYGADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLRADGSAFISD
NGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSKGE
LQAKKQTGKPFKKGTSICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIKE
YVEYMNAEKDSIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGKR
KDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKYMEFNPAILDLILEKAIQSFNAAE
AARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIYK
NTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGFIYIACPPLYKVERGKNH
NYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFI
ETHSGNLDMATLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=628, Percent_Identity=22.9299363057325, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI19913408, Length=619, Percent_Identity=24.3941841680129, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI48994957, Length=610, Percent_Identity=50.4918032786885, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1789408, Length=652, Percent_Identity=40.1840490797546, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI212645845, Length=547, Percent_Identity=25.9597806215722, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17535065, Length=575, Percent_Identity=22.7826086956522, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=635, Percent_Identity=25.8267716535433, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17136538, Length=574, Percent_Identity=25.609756097561, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73261; Mature: 73130

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKRSNKISNDYGADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLRADGSAFISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSKGELQAKKQTGKPFKKGTSICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVEYMNAEKDSIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKYMEFNPAILDLILEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSGNLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
SEDKRSNKISNDYGADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLRADGSAFISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSKGELQAKKQTGKPFKKGTSICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCHHHCCCEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNQLSDGSFKEEIYLYQGGIK
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVEYMNAEKDSIHPEIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKYMEFNPAILDLILEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRNFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDESSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGFIYIACPPLYKVERGKNHNYCYNENQLKDTIQGFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSGNLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]