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The map label for this gene is yjfH [C]

Identifier: 123969030

GI number: 123969030

Start: 1279617

End: 1280327

Strand: Reverse

Name: yjfH [C]

Synonym: A9601_14981

Alternate gene names: 123969030

Gene position: 1280327-1279617 (Counterclockwise)

Preceding gene: 123969034

Following gene: 123969029

Centisome position: 76.67

GC content: 33.47

Gene sequence:

>711_bases
TTGCTGAAGTCTGGAAAAAATCCTTCTAAGATTTTAGTTACTGAAAAATGGCTAAGAAAGAATCAAAATCTAAGCAAAAA
ATTTGATGAGTCATTAATAAATATTGTCTCAGAGGAAGTCTTGGCGTCAGCGATTTCAACAATTAATCCAGATGGGATAG
CAGCTTTAGTAGAGATTTCAGCAATACCTAATTATCAATTTAATAGAAAAGATGATTTTGTTCTTGTTCTCGACAGAATT
CAAGATCCTGGGAATATGGGTAATCTATTTAGAACCGCTTTAGCTGCGGGTGTTAATGCAATTTTTTTAGCTGGAGGTGC
GCACCCATTAGGCCAAAAGGTATTAAGAGCATCCTCAGGTGCAGTTTTTCATCTGCCATTTTTAAGATTTGATGGCATTG
AAGAAGAAATATTAAATTCTTTACTTAATTCTTTGTCTGAATTATCAAATGTAGGATTTAAGATTTTCTCTACTAGTAGC
CATAATGAGAGTACAAAAAAACCTTCAAAACCTTACTGGGAAGTTGATTGGTCTAGACGTACAGCATTAATTTTGGGTAA
TGAAGGTAAAGGTATTCATAAAAAAATTCAAGAAGCTTTTAATGAAACAATTACAATTCCGCATAGCGAGATTGTAGAAT
CATTGAATGTGGCTTGTGTTGCAGTTCCGTTATTACTTGAACGAAAAAGAGTCGCATATACCTCTAAATAA

Upstream 100 bases:

>100_bases
AAACAATAATCTAGTTAAAAGATTTAGATCATTTAAAAGAGGATCATCTCCAAAAGATCAAGACTTTTTTTGCATAGAGG
GTACACATCTCATTGAAGAA

Downstream 100 bases:

>100_bases
ATAAATAAAAAGTGACTGATTCAAGTTTTGATTTTGATTTAATTGTAATAGGAGCAGGATATGGAGGTTTTGATGCCGCT
AAACATGCTGCTGGTAAGGG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLKSGKNPSKILVTEKWLRKNQNLSKKFDESLINIVSEEVLASAISTINPDGIAALVEISAIPNYQFNRKDDFVLVLDRI
QDPGNMGNLFRTALAAGVNAIFLAGGAHPLGQKVLRASSGAVFHLPFLRFDGIEEEILNSLLNSLSELSNVGFKIFSTSS
HNESTKKPSKPYWEVDWSRRTALILGNEGKGIHKKIQEAFNETITIPHSEIVESLNVACVAVPLLLERKRVAYTSK

Sequences:

>Translated_236_residues
MLKSGKNPSKILVTEKWLRKNQNLSKKFDESLINIVSEEVLASAISTINPDGIAALVEISAIPNYQFNRKDDFVLVLDRI
QDPGNMGNLFRTALAAGVNAIFLAGGAHPLGQKVLRASSGAVFHLPFLRFDGIEEEILNSLLNSLSELSNVGFKIFSTSS
HNESTKKPSKPYWEVDWSRRTALILGNEGKGIHKKIQEAFNETITIPHSEIVESLNVACVAVPLLLERKRVAYTSK
>Mature_236_residues
MLKSGKNPSKILVTEKWLRKNQNLSKKFDESLINIVSEEVLASAISTINPDGIAALVEISAIPNYQFNRKDDFVLVLDRI
QDPGNMGNLFRTALAAGVNAIFLAGGAHPLGQKVLRASSGAVFHLPFLRFDGIEEEILNSLLNSLSELSNVGFKIFSTSS
HNESTKKPSKPYWEVDWSRRTALILGNEGKGIHKKIQEAFNETITIPHSEIVESLNVACVAVPLLLERKRVAYTSK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=170, Percent_Identity=28.2352941176471, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26055; Mature: 26055

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSGKNPSKILVTEKWLRKNQNLSKKFDESLINIVSEEVLASAISTINPDGIAALVEIS
CCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEE
AIPNYQFNRKDDFVLVLDRIQDPGNMGNLFRTALAAGVNAIFLAGGAHPLGQKVLRASSG
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCC
AVFHLPFLRFDGIEEEILNSLLNSLSELSNVGFKIFSTSSHNESTKKPSKPYWEVDWSRR
CEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEECCCCC
TALILGNEGKGIHKKIQEAFNETITIPHSEIVESLNVACVAVPLLLERKRVAYTSK
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKSGKNPSKILVTEKWLRKNQNLSKKFDESLINIVSEEVLASAISTINPDGIAALVEIS
CCCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEE
AIPNYQFNRKDDFVLVLDRIQDPGNMGNLFRTALAAGVNAIFLAGGAHPLGQKVLRASSG
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCC
AVFHLPFLRFDGIEEEILNSLLNSLSELSNVGFKIFSTSSHNESTKKPSKPYWEVDWSRR
CEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEECCCCC
TALILGNEGKGIHKKIQEAFNETITIPHSEIVESLNVACVAVPLLLERKRVAYTSK
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]