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The map label for this gene is hisH [H]

Identifier: 123968950

GI number: 123968950

Start: 1189724

End: 1190311

Strand: Reverse

Name: hisH [H]

Synonym: A9601_14171

Alternate gene names: 123968950

Gene position: 1190311-1189724 (Counterclockwise)

Preceding gene: 123968955

Following gene: 123968949

Centisome position: 71.28

GC content: 30.1

Gene sequence:

>588_bases
ATGCTGGATTCTATAGAAAATTGTGAGACAGTTATTGTAGAAAATTATCAAGATTTTAAATATACAGATATGCTTATTCT
CCCAGGCGTAGGTTCTTTTGATACTGGGATGAAACTATTGAAAAAACGTGAACTTTGTAAACCTTTAATTGATTATGCAA
ATAAAAAAAATCTTTTAGGTATATGCTTAGGTATGCAATTAATTTCAGAAGGTAGCGAGGAAGGAAGTGAAGAAGGTTTG
GGTATCATATCAGGATATTTTAAGAAGTTTCCTAAAGAAGATTTCAACTCAAAATCCTTAAAAGTACCTTGCATGGGTTG
GAATTTTGTAGAATATTCCTTAGAAAAGGGGTATCTATCCCATGAATATGCAATTAATGGAGTTACTAGATATTATTTTG
TACACTCTTATTATTATGCAGGACCAGAAGAAAATGTTTGCGGATGGAGCAATCATGGTATTAAATATGGTGTCGTTATA
AATAATAAAAAAACGATCGGGGTACAATTTCATCCTGAAAAAAGTCATGATTTTGGGAGAAAATTTTTAAAAAATTGGAT
AAATAAAAATTATGAAAAAACATCCTAG

Upstream 100 bases:

>100_bases
TAGATAGTTATTGATTAATAATTAATTGAGTATAGAAATCCCTCCTGTAAAAGTAGGTGTTATTAATATTGGCTTTGGAA
ATATTGGATCTTGCATTAAT

Downstream 100 bases:

>100_bases
AGTTATTTTATCTTTAATAATTGATAGTAAAAGAATGATAAAAAGATCAAAATTCAAAAATTACAAATATCTTGGAGATC
CATTAAATATAATAAGGATT

Product: hypothetical protein

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MLDSIENCETVIVENYQDFKYTDMLILPGVGSFDTGMKLLKKRELCKPLIDYANKKNLLGICLGMQLISEGSEEGSEEGL
GIISGYFKKFPKEDFNSKSLKVPCMGWNFVEYSLEKGYLSHEYAINGVTRYYFVHSYYYAGPEENVCGWSNHGIKYGVVI
NNKKTIGVQFHPEKSHDFGRKFLKNWINKNYEKTS

Sequences:

>Translated_195_residues
MLDSIENCETVIVENYQDFKYTDMLILPGVGSFDTGMKLLKKRELCKPLIDYANKKNLLGICLGMQLISEGSEEGSEEGL
GIISGYFKKFPKEDFNSKSLKVPCMGWNFVEYSLEKGYLSHEYAINGVTRYYFVHSYYYAGPEENVCGWSNHGIKYGVVI
NNKKTIGVQFHPEKSHDFGRKFLKNWINKNYEKTS
>Mature_195_residues
MLDSIENCETVIVENYQDFKYTDMLILPGVGSFDTGMKLLKKRELCKPLIDYANKKNLLGICLGMQLISEGSEEGSEEGL
GIISGYFKKFPKEDFNSKSLKVPCMGWNFVEYSLEKGYLSHEYAINGVTRYYFVHSYYYAGPEENVCGWSNHGIKYGVVI
NNKKTIGVQFHPEKSHDFGRKFLKNWINKNYEKTS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=172, Percent_Identity=38.953488372093, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=196, Percent_Identity=31.1224489795918, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22411; Mature: 22411

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSIENCETVIVENYQDFKYTDMLILPGVGSFDTGMKLLKKRELCKPLIDYANKKNLLG
CCCCCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ICLGMQLISEGSEEGSEEGLGIISGYFKKFPKEDFNSKSLKVPCMGWNFVEYSLEKGYLS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHEEHHHCCCCC
HEYAINGVTRYYFVHSYYYAGPEENVCGWSNHGIKYGVVINNKKTIGVQFHPEKSHDFGR
CHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCHHHHH
KFLKNWINKNYEKTS
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MLDSIENCETVIVENYQDFKYTDMLILPGVGSFDTGMKLLKKRELCKPLIDYANKKNLLG
CCCCCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ICLGMQLISEGSEEGSEEGLGIISGYFKKFPKEDFNSKSLKVPCMGWNFVEYSLEKGYLS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHEEHHHCCCCC
HEYAINGVTRYYFVHSYYYAGPEENVCGWSNHGIKYGVVINNKKTIGVQFHPEKSHDFGR
CHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCHHHHH
KFLKNWINKNYEKTS
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA