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The map label for this gene is hisF [H]

Identifier: 123968949

GI number: 123968949

Start: 1188976

End: 1189740

Strand: Reverse

Name: hisF [H]

Synonym: A9601_14161

Alternate gene names: 123968949

Gene position: 1189740-1188976 (Counterclockwise)

Preceding gene: 123968950

Following gene: 123968948

Centisome position: 71.25

GC content: 26.67

Gene sequence:

>765_bases
ATGAAAAAACATCCTAGAGTTATTTTATCTTTAATAATTGATAGTAAAAGAATGATAAAAAGATCAAAATTCAAAAATTA
CAAATATCTTGGAGATCCATTAAATATAATAAGGATTTTTAATTCTAAAAGAGTTGATGAAATATCCATAATTTCTTGTG
AAAATTATGAAAAGAAAGATATTAATATTGATTTTGAATTCCTGGAAAAAATTTCTGGGGAAGCATTTTGTCCATTAACT
TATTCAGGAGGTGTAAGAAGTTTAGAAGATGCTGATAAGCTTTTTTCTATAGGTTTTGAGAAGATTGGGATTAGAAATCT
TCTTCATACTAATCCAGAAGTAATAAAACAAATTGCTGAAAAATATGGTTCCCAATCCGTTGTTATTTTTATTGATTATA
AAGAATTAAATAATAATGAAATTGAAATTTATACTTCATATAATTCATTTTTCAAATCAACAAAATCTTCAATTCTTGAA
TTTATAAAAAATGCAGAATCATATGGAGCTGGTGAGGTGGTTTTACAATCTATCCTTAGAGAAGGCCAATCAAAGGGTCT
AGATTTAGATATTATTAAAGAGATTACAAATAAAACAATACTTCCAATTATTTCATCTTGTGGAATGAGAGATTTATCTG
ATGCTAGAAAGGCATTCTCATTAGGAGCTGATGGAGTTATGGCAAGTAAGTTTTTTTCTCTTTATGGGAATTGTGATGGA
GTATTATTGACTTATCCAACCTATCAGGATATTGAAAAACTTTAA

Upstream 100 bases:

>100_bases
GTCGTTATAAATAATAAAAAAACGATCGGGGTACAATTTCATCCTGAAAAAAGTCATGATTTTGGGAGAAAATTTTTAAA
AAATTGGATAAATAAAAATT

Downstream 100 bases:

>100_bases
TGGCAAAGGAATGTGTAAATTGTTTATTAAATGATTCTTTAAGCAATGTATTTATTGATAATTCTAATATTTGTAATTTT
TGTAAGTCTTATTCAAAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKKHPRVILSLIIDSKRMIKRSKFKNYKYLGDPLNIIRIFNSKRVDEISIISCENYEKKDINIDFEFLEKISGEAFCPLT
YSGGVRSLEDADKLFSIGFEKIGIRNLLHTNPEVIKQIAEKYGSQSVVIFIDYKELNNNEIEIYTSYNSFFKSTKSSILE
FIKNAESYGAGEVVLQSILREGQSKGLDLDIIKEITNKTILPIISSCGMRDLSDARKAFSLGADGVMASKFFSLYGNCDG
VLLTYPTYQDIEKL

Sequences:

>Translated_254_residues
MKKHPRVILSLIIDSKRMIKRSKFKNYKYLGDPLNIIRIFNSKRVDEISIISCENYEKKDINIDFEFLEKISGEAFCPLT
YSGGVRSLEDADKLFSIGFEKIGIRNLLHTNPEVIKQIAEKYGSQSVVIFIDYKELNNNEIEIYTSYNSFFKSTKSSILE
FIKNAESYGAGEVVLQSILREGQSKGLDLDIIKEITNKTILPIISSCGMRDLSDARKAFSLGADGVMASKFFSLYGNCDG
VLLTYPTYQDIEKL
>Mature_254_residues
MKKHPRVILSLIIDSKRMIKRSKFKNYKYLGDPLNIIRIFNSKRVDEISIISCENYEKKDINIDFEFLEKISGEAFCPLT
YSGGVRSLEDADKLFSIGFEKIGIRNLLHTNPEVIKQIAEKYGSQSVVIFIDYKELNNNEIEIYTSYNSFFKSTKSSILE
FIKNAESYGAGEVVLQSILREGQSKGLDLDIIKEITNKTILPIISSCGMRDLSDARKAFSLGADGVMASKFFSLYGNCDG
VLLTYPTYQDIEKL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=231, Percent_Identity=25.974025974026, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=270, Percent_Identity=23.7037037037037, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28856; Mature: 28856

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHPRVILSLIIDSKRMIKRSKFKNYKYLGDPLNIIRIFNSKRVDEISIISCENYEKKD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCEEEEEECCCCCCCC
INIDFEFLEKISGEAFCPLTYSGGVRSLEDADKLFSIGFEKIGIRNLLHTNPEVIKQIAE
CCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KYGSQSVVIFIDYKELNNNEIEIYTSYNSFFKSTKSSILEFIKNAESYGAGEVVLQSILR
HHCCCEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGQSKGLDLDIIKEITNKTILPIISSCGMRDLSDARKAFSLGADGVMASKFFSLYGNCDG
HHHCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
VLLTYPTYQDIEKL
EEEECCCHHHHHCC
>Mature Secondary Structure
MKKHPRVILSLIIDSKRMIKRSKFKNYKYLGDPLNIIRIFNSKRVDEISIISCENYEKKD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCEEEEEECCCCCCCC
INIDFEFLEKISGEAFCPLTYSGGVRSLEDADKLFSIGFEKIGIRNLLHTNPEVIKQIAE
CCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KYGSQSVVIFIDYKELNNNEIEIYTSYNSFFKSTKSSILEFIKNAESYGAGEVVLQSILR
HHCCCEEEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGQSKGLDLDIIKEITNKTILPIISSCGMRDLSDARKAFSLGADGVMASKFFSLYGNCDG
HHHCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
VLLTYPTYQDIEKL
EEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10474199 [H]