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The map label for this gene is fmt [C]

Identifier: 123968876

GI number: 123968876

Start: 1118699

End: 1119496

Strand: Direct

Name: fmt [C]

Synonym: A9601_13431

Alternate gene names: 123968876

Gene position: 1118699-1119496 (Clockwise)

Preceding gene: 123968875

Following gene: 123968877

Centisome position: 66.99

GC content: 29.57

Gene sequence:

>798_bases
ATGACAATAGTTTTAATAACGGGGACTCAAAATAGACACCTAAGTATAGCTTCAAAACTTATAGATAATTTTGAAGTTAT
TTGGATTAGATATAAAAGAAAATTAGTTCCTCAAGTTGATCAAATTCAGTCGAATTTTCTTAAAGAGCATCTTGAAAAAC
TTGTTAAAGATGAGACAGATGAGTTAGGTATTTTTAATATAAATGAGATTCTCAAGAGTAAGAATATTATTAAAAGTTTG
GAAATCGATTCTTTAAAGAATTTCAATGAGTTATCTCCAAAGTTTCTAGAAAGTTTTGAATTTAATGCAGATGCTACTGT
TATATATGGGAGCGGTATAATAGGTGATGATTTCATGAAAGTCCTGCCACAACCTCTTATAAATATCCATGGCGGGATTT
CGCCATATTTCAAAGGTTCAAGTACACTTTTGTACGCTTTAGCCTTAGGTCAACCTGAACTATTAGGGATGACGATTCAT
GAGATTGATTCTGGCATAGATTCGGGAGATATATTTTGTCACTTGCTTCCTGATCTTAAGTTTGGGATGAGTCCAACAAA
AATGTTTGCATCTTGTCAAAAAAAATTATACGAAGAAATTGAAAATATTATTAATAAAATAATAGAAGGGAGCTTGACTT
CAGTGCCACAATCTAAGTTTGGAAGAACATTTATGGAAAGAGATTATAGAGAAGCTATTCTTAAAAGTATTTACACAATG
TATGAAGAAGGTTTTTTTAATTTATCAATTTCTGAATTAAAGAAATTAAGAAATAAATATAAATTAAGAGAATGTTAG

Upstream 100 bases:

>100_bases
AAAAGGTGTGGTCTCATCTGAGTATTCAAGGCTAGATATTGATACAATGCTTGACTTTAGATTTATCGAAAGCTTATTAA
AAATAGTCAAATAGAATTTA

Downstream 100 bases:

>100_bases
AAGTAACTTTATTAGGAAATGGGCCTTCATTATCTAATATAAATTTTGGAGAAACTATTAATAAAAGTAATTTTATAATT
AGTTGTAATAGAATTTTCCT

Product: hypothetical protein

Products: tetrahydrofolate; N-formylmethionyl-tRNA [C]

Alternate protein names: Formyl Transferase Domain-Containing Protein

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTIVLITGTQNRHLSIASKLIDNFEVIWIRYKRKLVPQVDQIQSNFLKEHLEKLVKDETDELGIFNINEILKSKNIIKSL
EIDSLKNFNELSPKFLESFEFNADATVIYGSGIIGDDFMKVLPQPLINIHGGISPYFKGSSTLLYALALGQPELLGMTIH
EIDSGIDSGDIFCHLLPDLKFGMSPTKMFASCQKKLYEEIENIINKIIEGSLTSVPQSKFGRTFMERDYREAILKSIYTM
YEEGFFNLSISELKKLRNKYKLREC

Sequences:

>Translated_265_residues
MTIVLITGTQNRHLSIASKLIDNFEVIWIRYKRKLVPQVDQIQSNFLKEHLEKLVKDETDELGIFNINEILKSKNIIKSL
EIDSLKNFNELSPKFLESFEFNADATVIYGSGIIGDDFMKVLPQPLINIHGGISPYFKGSSTLLYALALGQPELLGMTIH
EIDSGIDSGDIFCHLLPDLKFGMSPTKMFASCQKKLYEEIENIINKIIEGSLTSVPQSKFGRTFMERDYREAILKSIYTM
YEEGFFNLSISELKKLRNKYKLREC
>Mature_264_residues
TIVLITGTQNRHLSIASKLIDNFEVIWIRYKRKLVPQVDQIQSNFLKEHLEKLVKDETDELGIFNINEILKSKNIIKSLE
IDSLKNFNELSPKFLESFEFNADATVIYGSGIIGDDFMKVLPQPLINIHGGISPYFKGSSTLLYALALGQPELLGMTIHE
IDSGIDSGDIFCHLLPDLKFGMSPTKMFASCQKKLYEEIENIINKIIEGSLTSVPQSKFGRTFMERDYREAILKSIYTMY
EEGFFNLSISELKKLRNKYKLREC

Specific function: Modifies The Free Amino Group Of The Aminoacyl Moiety Of Methionyl-Trna(Fmet). The Formyl Group Appears To Play A Dual Role In The Initiator Identity Of N-Formylmethionyl-Trna By: (I) Promoting Its Recognition By If2 And (II) Impairing Its Binding To Eftu

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.2.9 [C]

Molecular weight: Translated: 30348; Mature: 30216

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVLITGTQNRHLSIASKLIDNFEVIWIRYKRKLVPQVDQIQSNFLKEHLEKLVKDETD
CEEEEEECCCCCHHHHHHHHHCCHHEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
ELGIFNINEILKSKNIIKSLEIDSLKNFNELSPKFLESFEFNADATVIYGSGIIGDDFMK
CCCCEEHHHHHHHHHHHHHHCHHHHCCCHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHH
VLPQPLINIHGGISPYFKGSSTLLYALALGQPELLGMTIHEIDSGIDSGDIFCHLLPDLK
HCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCHH
FGMSPTKMFASCQKKLYEEIENIINKIIEGSLTSVPQSKFGRTFMERDYREAILKSIYTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YEEGFFNLSISELKKLRNKYKLREC
HHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIVLITGTQNRHLSIASKLIDNFEVIWIRYKRKLVPQVDQIQSNFLKEHLEKLVKDETD
EEEEEECCCCCHHHHHHHHHCCHHEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
ELGIFNINEILKSKNIIKSLEIDSLKNFNELSPKFLESFEFNADATVIYGSGIIGDDFMK
CCCCEEHHHHHHHHHHHHHHCHHHHCCCHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHH
VLPQPLINIHGGISPYFKGSSTLLYALALGQPELLGMTIHEIDSGIDSGDIFCHLLPDLK
HCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCHH
FGMSPTKMFASCQKKLYEEIENIINKIIEGSLTSVPQSKFGRTFMERDYREAILKSIYTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YEEGFFNLSISELKKLRNKYKLREC
HHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; K+; Mg2+; Na+; NH4+ [C]

Kcat value (1/min): 1200 [C]

Specific activity: 1.14

Km value (mM): 0.013 {10-formyltetrahydrofolate}} 0.0013 {phenylalanyl-tRNAfMet}} 0.0014 {valyl-tRNAfMet}} 0.0006 {methionyl-tRNAfMet}} 0.00035 {methionyl-tRNAfMet}} [C]

Substrates: Formyltetrahydrofolate; L-methionyl-tRNA; H2O [C]

Specific reaction: Formyltetrahydrofolate + L-methionyl-tRNA + H2O =tetrahydrofolate + N-formylmethionyl-tRNA [C]

General reaction: Formyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA