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The map label for this gene is pdhB [H]

Identifier: 123968463

GI number: 123968463

Start: 799701

End: 800684

Strand: Direct

Name: pdhB [H]

Synonym: A9601_09301

Alternate gene names: 123968463

Gene position: 799701-800684 (Clockwise)

Preceding gene: 123968462

Following gene: 123968464

Centisome position: 47.89

GC content: 34.45

Gene sequence:

>984_bases
GTGGCTGGAACATTATTATTTAATGCTTTGAAAGAGGCAATTGATGAAGAAATGGCAAATGATGTAAATGTTTGCGTAAT
GGGGGAAGATGTTGGTCAATATGGAGGATCTTATAAGGTAACTAAGGATTTATATGAAAAATATGGAGAGTTAAGAGTCT
TAGATACTCCAATTGCAGAGAATAGTTTTACGGGTATGGCTGTGGGTGCAGCAATGACTGGCTTAAGACCAATAGTAGAA
GGAATGAATATGGGGTTTTTGCTTTTAGCATTTAATCAGATATCAAACAATATGGGTATGCTTAGATATACAAGTGGCGG
AAATTATAAAATACCAGCAGTAGTTCGAGGACCTGGTGGAGTTGGTCGTCAACTTGGTGCTGAGCATAGTCAAAGACTTG
AAGCATATTTTCATGCAGTTCCTGGCATAAAGATTGTTGCATGTAGTACACCCACAAATGCTAAAGGTTTAATGAAAGCA
GCTATAAGAGATGATAATCCGGTTCTATTTTTTGAACATGTTCTTCTATACAATTTGTCTGAAGAATTACCTGAGGGTGA
TTATACTTGCGCTTTAGATCAGGCTGACGTTGTAAAAGAAGGGCGTGATATTACTTTATTGACTTATTCAAGAATGAGAC
ATCACTGCCTTAAAGCTGTTGAAGAATTAGAAAAAAAAGGAATAGATGTTGAGTTAATAGATTTAATAAGTTTAAAACCA
TTTGATATGGAAACCATCTCAAAATCAATAAAAAAAACAAATAAAGTAATTATTGTTGAAGAATGTATGAAGACTGGAGG
TATTGGTGCAGAATTAATTGCCTTGATAACAGAAGAGTGTTTTGATGATCTTGATGCCCGACCAATTAGATTATCTAGTC
AGGATATTCCAACTCCCTATAATGGAAATCTTGAGAATTTGACAATAATCCAACCACATCAAATTGTTGAAAAAGTTGAA
CATTTAATTAGTGGGAGTATATAG

Upstream 100 bases:

>100_bases
GGAATGATTTTATCAATATTACTACTCATAACCTTCGCTGAAGGAGGCTGATTTATTCAAAGTGGTAGGATTATATATGT
GATAAATTAGGTAATTAATT

Downstream 100 bases:

>100_bases
AAAATGAAAAGAAGGCAAGGTTGGCTTTTTTTTATTATATTTCTACTTACTTTATCTGTTTATCTATTAATAAATTATCC
ATTACAGTTGGGATTGGATT

Product: pyruvate dehydrogenase E1 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGRDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
FDMETISKSIKKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
HLISGSI

Sequences:

>Translated_327_residues
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGRDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
FDMETISKSIKKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVE
HLISGSI
>Mature_326_residues
AGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVEG
MNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAA
IRDDNPVLFFEHVLLYNLSEELPEGDYTCALDQADVVKEGRDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKPF
DMETISKSIKKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPYNGNLENLTIIQPHQIVEKVEH
LISGSI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=314, Percent_Identity=41.0828025477707, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI291084858, Length=314, Percent_Identity=38.8535031847134, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4557353, Length=317, Percent_Identity=36.5930599369085, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI34101272, Length=317, Percent_Identity=36.5930599369085, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17538422, Length=318, Percent_Identity=41.5094339622642, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17506935, Length=298, Percent_Identity=35.5704697986577, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319698, Length=322, Percent_Identity=43.167701863354, Blast_Score=268, Evalue=8e-73,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=39.3846153846154, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=39.3846153846154, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI160714832, Length=299, Percent_Identity=39.1304347826087, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI160714828, Length=299, Percent_Identity=39.1304347826087, Blast_Score=204, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24650943, Length=85, Percent_Identity=51.7647058823529, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24650945, Length=85, Percent_Identity=51.7647058823529, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35916; Mature: 35785

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDDNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPEGDYTCALDQADVVKEGRDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
HHCCCCCCEEECCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDMETISKSIKKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPY
CCHHHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEHLISGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGTLLFNALKEAIDEEMANDVNVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNYKIPAVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEEEEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDDNPVLFFEHVLLYNLS
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPEGDYTCALDQADVVKEGRDITLLTYSRMRHHCLKAVEELEKKGIDVELIDLISLKP
HHCCCCCCEEECCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
FDMETISKSIKKTNKVIIVEECMKTGGIGAELIALITEECFDDLDARPIRLSSQDIPTPY
CCHHHHHHHHHHHCCEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
NGNLENLTIIQPHQIVEKVEHLISGSI
CCCCCEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]