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The map label for this gene is lig [H]

Identifier: 123968318

GI number: 123968318

Start: 680538

End: 682178

Strand: Reverse

Name: lig [H]

Synonym: A9601_07831

Alternate gene names: 123968318

Gene position: 682178-680538 (Counterclockwise)

Preceding gene: 123968319

Following gene: 123968317

Centisome position: 40.85

GC content: 26.81

Gene sequence:

>1641_bases
ATGAGCTTAAAAAAGTTTTCAGAATTATTTGGCGATCTAGATTCAATTAATAGTACAAATAATAAAATTGAAGTTTTAAA
GAATTATTTTTTATCTAATGATGCAATAGATAATTCATGGGCAATATATTTACTAACTGGAAAAAGTAATAAGAGATTTA
TTAGTGGAAGATATTTAAAAAATCTTTTTTCTCAAATATATGAATATCCTCAATGGTTAATTGATACATGTTATTTAAAA
GTTGGAGATTCTGCTGAGGTAATAACGTTATTACTTAAAAACAAAACTAATTCTAGAAAAAAGAAATTATCAAATATAAG
TCTCAATGAATTACTAAGCGAAACAATACCTGCATTATCAAAACTTAATGAGGAAGAGAAAAATTTAGAAATTAAAAATC
TTTGGGAAACATTACCTGAAGATAACCATCTAATTTTTAATAAAATTCTTACAGGAACTTTTAGAGTAGGAGTCTCTATC
GGATTAATAACAAAATCAATATCAAAACTAATTAATATTGAGGAAGAGATTATTTCTCATAGGTTGATGGGTGATTTTAA
ACCTTCAATTGATTCATATGAATTTTTAATTAACAAGAATATCAATCTTCAAGAGTTAAATTCCAAACCATTTCCATTTC
TTCTAGCAAATACTATTGAAGATAAAATCTTCAAAAATTCAATAAATGATTTTCAATTTGAATGGAAATACGACGGTATA
AGAATGCAATTAATTAAAAGATCAGGCAATGTTTCGTTATGGACAAGAGGGCAAGAATTAGTAAATGAATCTTTCCCAGA
ATTAGTAGAGAAAATGTCATATATAAAAGATGATTTTGTTCTTGATGGGGAATTATTAGTTTGGAATTTTAAAGAACAAA
TTGCCTTTGATTTTTCTTTACTTCAAAAAAGGATAAATAGAAAGTCTCCTACTAGATCAATCCAAATAAAATATCCAATT
ATTTTTATTGCTTATGATCTTTTAGAGATTAATGGGAGAGATATAAGAGAAATCAAATTAGAGAATAGAAGAATTGAGTT
AGAAAAATATTTTTCAAAATGGCAAATTAAATCTGAGAATAATATCTCTGATATTTTCAAAATATGTGATTTAATCTTTC
CTAAAGATTGGCCTGATGCTTTAACTTATAAAGAAAAATCTCGAGAAAATAATACTGAAGGATTAATAATTAAGAAAAAG
ACTTCTATATACTCCTCAGGTAGAAAGAAAGGTTTTTGGTGGAAATATAAAGTTGATCCTATGCAACTGGATGCTGTTCT
AATTTACGCTAAGGGCGGTAGCGGTAGAAGGGCTGGTCTGTATACAGATTACAGTTTTGCATTATGGAAAGACAAAGAAT
TAATTAAATTTGCAAGTGCATACTCTGGTTTAACGAATATTGAGATTAAAGAGCTAGATAAATGGATAAGAAAAAATACA
ATAGAAAAATTTGGTCCTGTTCGATCGTTAAAACCAGAAATGGTATTCGAAATATCTTTTGAGAAAATACAAATTTCAAA
TCGTCATAAGTCAGGTATAGCAGTAAGATTTCCAAGGATAACAAAATGGAGAAAAGATAAAAAAATTAATGATGCAGATA
GCATTGAGAATGCTTATGAACTAATGAAGAAAATATCATGA

Upstream 100 bases:

>100_bases
AAGCAAAAAATGTATTTTTTCATCATGGAGATAGTGAAGCCTTAAGTAAATATTTGGTTGAAAAGGAATCAATAAATGTA
CTTTTATTCGGTAAATAAAT

Downstream 100 bases:

>100_bases
AAAATATTACGAAAAAGAGTAAGCAAAATAATTTAATTTCTAAAATTAAACAGTTTTTCTCCACAAATGGATGGGAGCCA
CTACCCTATCAGATCGAATC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDAIDNSWAIYLLTGKSNKRFISGRYLKNLFSQIYEYPQWLIDTCYLK
VGDSAEVITLLLKNKTNSRKKKLSNISLNELLSETIPALSKLNEEEKNLEIKNLWETLPEDNHLIFNKILTGTFRVGVSI
GLITKSISKLINIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSYIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTRSIQIKYPI
IFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKSENNISDIFKICDLIFPKDWPDALTYKEKSRENNTEGLIIKKK
TSIYSSGRKKGFWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKINDADSIENAYELMKKIS

Sequences:

>Translated_546_residues
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDAIDNSWAIYLLTGKSNKRFISGRYLKNLFSQIYEYPQWLIDTCYLK
VGDSAEVITLLLKNKTNSRKKKLSNISLNELLSETIPALSKLNEEEKNLEIKNLWETLPEDNHLIFNKILTGTFRVGVSI
GLITKSISKLINIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSYIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTRSIQIKYPI
IFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKSENNISDIFKICDLIFPKDWPDALTYKEKSRENNTEGLIIKKK
TSIYSSGRKKGFWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKINDADSIENAYELMKKIS
>Mature_545_residues
SLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDAIDNSWAIYLLTGKSNKRFISGRYLKNLFSQIYEYPQWLIDTCYLKV
GDSAEVITLLLKNKTNSRKKKLSNISLNELLSETIPALSKLNEEEKNLEIKNLWETLPEDNHLIFNKILTGTFRVGVSIG
LITKSISKLINIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGIR
MQLIKRSGNVSLWTRGQELVNESFPELVEKMSYIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTRSIQIKYPII
FIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKSENNISDIFKICDLIFPKDWPDALTYKEKSRENNTEGLIIKKKT
SIYSSGRKKGFWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNTI
EKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKINDADSIENAYELMKKIS

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=349, Percent_Identity=25.5014326647564, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985269, Length=370, Percent_Identity=28.1081081081081, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320038, Length=347, Percent_Identity=22.1902017291066, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762424, Length=354, Percent_Identity=23.728813559322, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 64027; Mature: 63895

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDAIDNSWAIYLLTGKSNKRFISGRYLK
CCHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECHHHHH
NLFSQIYEYPQWLIDTCYLKVGDSAEVITLLLKNKTNSRKKKLSNISLNELLSETIPALS
HHHHHHHHHHHHHHHHHHEEECCCHHEEHEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHH
KLNEEEKNLEIKNLWETLPEDNHLIFNKILTGTFRVGVSIGLITKSISKLINIEEEIISH
HCCCHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEHHHHHHHHHHHHCHHHHHHHH
RLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
HHHCCCCCCCCCEEEEEECCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCE
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSYIKDDFVLDGELLVWNFKEQIAFDFSL
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECHHHHHHHHHH
LQKRINRKSPTRSIQIKYPIIFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKSEN
HHHHHCCCCCCCEEEEECCEEEEEEEHHHCCCCCEEEEEECCCEEHHHHHHHHCEECCCC
NISDIFKICDLIFPKDWPDALTYKEKSRENNTEGLIIKKKTSIYSSGRKKGFWWKYKVDP
CHHHHHHHHHHHCCCCCCCHHCHHHHCCCCCCCEEEEEECHHHHHCCCCCCCEEEEECCC
MQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
EEEEEEEEEEECCCCCCCCEEECCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKINDADSIENAYE
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHH
LMKKIS
HHHHCC
>Mature Secondary Structure 
SLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDAIDNSWAIYLLTGKSNKRFISGRYLK
CHHHHHHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECHHHHH
NLFSQIYEYPQWLIDTCYLKVGDSAEVITLLLKNKTNSRKKKLSNISLNELLSETIPALS
HHHHHHHHHHHHHHHHHHEEECCCHHEEHEEHHCCCCHHHHHHHCCCHHHHHHHHHHHHH
KLNEEEKNLEIKNLWETLPEDNHLIFNKILTGTFRVGVSIGLITKSISKLINIEEEIISH
HCCCHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEHHHHHHHHHHHHCHHHHHHHH
RLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
HHHCCCCCCCCCEEEEEECCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCE
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSYIKDDFVLDGELLVWNFKEQIAFDFSL
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECHHHHHHHHHH
LQKRINRKSPTRSIQIKYPIIFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKSEN
HHHHHCCCCCCCEEEEECCEEEEEEEHHHCCCCCEEEEEECCCEEHHHHHHHHCEECCCC
NISDIFKICDLIFPKDWPDALTYKEKSRENNTEGLIIKKKTSIYSSGRKKGFWWKYKVDP
CHHHHHHHHHHHCCCCCCCHHCHHHHCCCCCCCEEEEEECHHHHHCCCCCCCEEEEECCC
MQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
EEEEEEEEEEECCCCCCCCEEECCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKINDADSIENAYE
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHH
LMKKIS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA