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The map label for this gene is hisF [H]

Identifier: 123968022

GI number: 123968022

Start: 420410

End: 421180

Strand: Direct

Name: hisF [H]

Synonym: A9601_04851

Alternate gene names: 123968022

Gene position: 420410-421180 (Clockwise)

Preceding gene: 123968018

Following gene: 123968023

Centisome position: 25.18

GC content: 36.19

Gene sequence:

>771_bases
ATGGTAGCTCTTCGTTTAATTCCTTGTTTAGATGTCGCCCATGGCAGAGTGGTTAAAGGTGTAAATTTTGTTAATTTGAG
AGACTCAGGCGATCCTGTTGAATTGGCTTGTAGGTATTCTGATGAGGGAGCAGATGAATTAGTATTCTTAGATATTAGAG
CTAGTGTAGAAAATAGAAATACATTAGTTGACCTTGTCTCTAGGACCGCAAAATCAGTAAAAATCCCATTTACAGTAGGT
GGAGGAATAGATTCTGTTTCTTCAATTAATGATCTTTTAAGAGCTGGAGCGGATAAAGTAAGTTTGAATTCTTCTGCAGT
TAGAAATCCAGATTTAATTTCTAAAAGTTCTAGAGAATTTGGCAATCAATGTATTGTGATAGCAATTGATGCTCGAAGAA
AAGTTAATAAGTTTGGTGAGTGGGAGGTATATGTAAAAGGAGGGCGAGAAAATACTGGTATAGATGTATTAAGTTGGGCA
AAGAAAGTTGAGGAGTTAGGCGCGGGGGAAATATTGCTTACTTCAATGGATGGTGATGGAACACAGAATGGATATGATTT
GAATCTGACCGAATCTGTTGCAAATATTGTTGATATCCCAGTAATTGCTTCTGGAGGAGCAGGCTCTCTAGAAGATATCT
TTGATGTTTTCAAAGAAGGCAGGGCATCAGCAGCACTTTTAGCATCATTACTTCATGATAAGAAACTTACTTTAAAAGAA
ATAAAAACTTTCCTCCTCGAAAAAAAACTCTCAATTAGACCATATGAATAA

Upstream 100 bases:

>100_bases
ATTTTACTACCGATTTTGGGTAATAATTTTGTTTCAGCCATAAGAGGTAAAATGGAAGCTATTATGCAAGATTAACAGCA
TTTGGACAATTTTTACTAAA

Downstream 100 bases:

>100_bases
AAATTTTAATTAAATCCCAAAAAAATAAGTTAAAAATTTAAAAATGAAATTTACAAAAACTATCGAAGTCAAAAATATAT
TTAATAAAATTTCCTATAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDARRKVNKFGEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLNLTESVANIVDIPVIASGGAGSLEDIFDVFKEGRASAALLASLLHDKKLTLKE
IKTFLLEKKLSIRPYE

Sequences:

>Translated_256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDARRKVNKFGEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLNLTESVANIVDIPVIASGGAGSLEDIFDVFKEGRASAALLASLLHDKKLTLKE
IKTFLLEKKLSIRPYE
>Mature_256_residues
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRNTLVDLVSRTAKSVKIPFTVG
GGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREFGNQCIVIAIDARRKVNKFGEWEVYVKGGRENTGIDVLSWA
KKVEELGAGEILLTSMDGDGTQNGYDLNLTESVANIVDIPVIASGGAGSLEDIFDVFKEGRASAALLASLLHDKKLTLKE
IKTFLLEKKLSIRPYE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.579766536965, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=28.8702928870293, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=33.5483870967742, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27693; Mature: 27693

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRN
CCEEEEECHHHCCCCCEECCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCH
TLVDLVSRTAKSVKIPFTVGGGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREF
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCHHHH
GNQCIVIAIDARRKVNKFGEWEVYVKGGRENTGIDVLSWAKKVEELGAGEILLTSMDGDG
CCEEEEEEECCCHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLNLTESVANIVDIPVIASGGAGSLEDIFDVFKEGRASAALLASLLHDKKLTLKE
CCCCEEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
IKTFLLEKKLSIRPYE
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVALRLIPCLDVAHGRVVKGVNFVNLRDSGDPVELACRYSDEGADELVFLDIRASVENRN
CCEEEEECHHHCCCCCEECCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCH
TLVDLVSRTAKSVKIPFTVGGGIDSVSSINDLLRAGADKVSLNSSAVRNPDLISKSSREF
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCHHHH
GNQCIVIAIDARRKVNKFGEWEVYVKGGRENTGIDVLSWAKKVEELGAGEILLTSMDGDG
CCEEEEEEECCCHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLNLTESVANIVDIPVIASGGAGSLEDIFDVFKEGRASAALLASLLHDKKLTLKE
CCCCEEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
IKTFLLEKKLSIRPYE
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA