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The map label for this gene is lepA [H]

Identifier: 123968008

GI number: 123968008

Start: 409437

End: 411245

Strand: Direct

Name: lepA [H]

Synonym: A9601_04711

Alternate gene names: 123968008

Gene position: 409437-411245 (Clockwise)

Preceding gene: 123968007

Following gene: 123968009

Centisome position: 24.52

GC content: 34.38

Gene sequence:

>1809_bases
ATGACTGATATATCGGTTTCAAAAATTAGAAATTTCTGCATAATCGCTCATATTGACCATGGTAAATCTACCCTTGCAGA
TAGGTTGCTTCAAGATACTGGTACTGTGCAGCAAAGGGATATGCAAGAACAATTTTTGGACAGTATGGATCTTGAAAGAG
AGAGAGGAATTACTATCAAGTTACAGGCCGCTAGGATGAAATATAAAGCTGACGATTCCCAAGAATATGTTTTGAACTTA
ATAGATACTCCAGGGCATGTTGATTTCTCTTATGAGGTTAGTAGATCTCTTCAAGCTTGTGAAGGCGCTTTACTCGTTGT
TGATGCAAGTCAAGGAGTAGAAGCTCAAACCTTAGCTAATGTTTATCTTGCCTTAGAAAATAATCTTGAAATTATTCCGG
TCTTAAATAAAGTTGATTTGCCAGGTGCTGATGCTGAAAAAATAAAACAAGAAATAGAGGAAATTATTGGACTTGATACA
TCTAATGCAATAAATTGTTCAGCAAAAACTGGAGTTGGTATTAAAGATATTTTGGAAGCAATCGTAAGAAGAGTACCTCC
TCCTCAAGATGAAATTAAACTACCTACAAAGGCACTCATTTTCGATTCTTATTATGATCCGTACAGGGGAGTTATTGTTT
ATTTCAGAGTGATATCTGGGTCTTTAAATAAGAGAGAAAAAATATTATTAATGGCAAGTAAGAAAAATTATGAACTAGAT
GAAATAGGAATAATGGCGCCTGATCAGCAGCAAGTTGATGAATTACATGCAGGAGAAGTTGGTTATTTAGCTGCTTCTAT
AAAATCAGTTGCTGATGCGAGAGTGGGAGATACGATTACTCTTTTAAATTCACCTGCCAATGATCCTTTGCCTGGATATA
AGACAGCAAATCCTATGGTTTTTTGTGGCTTATTCCCGACAGATGCTGATCAATTCCCAGATTTAAGAGTATCACTCGAA
AAATTACAATTATCTGATGCAGCTTTAAAATATGAGCCCGAAACCAGTAGCGCAATGGGCTTCGGATTTAGGTGCGGATT
CCTAGGACTTCTTCATATGGAGATTGTTCAAGAAAGATTAGAAAGAGAATATGACTTGGATTTAATCGTAACGGCACCAT
CAGTTATTTATAAAGTTAATTTAAATCAGCAGGAACATATCTTTATTGATAATCCTTCTACAATTCCTGATCCACAACTT
AGGGAATCAATAGAAGAGCCTTATGTGAAAATGGAAATTTATGCTCCCAATGAATTTAATGGAACATTAATGGGTTTATG
TCAGGAAAGAAGGGGTGTATTTATAGATATGAAATACATAACAACAGATCGAGTTACCTTGATTTATGAAATTCCATTAG
CAGAAGTTGTTACAGATTTCTTTGATCAAATGAAAAGTAGAACCCAAGGTTATGCATCAATGGAATATCATTTGATTGGC
TATAGAAAAAATGACCTTGTTAGATTAGATGTTCTAATAAATTCAGAAAGAGCCGATCCATTAACTTCTATTGTTCATAA
AGATAAGGCTTATGGAATTGGCAGAAGTTTAGTTGAGAAATTAAAAGAACTTATTCCAAAACAACAATTTAAAATACCTA
TTCAAGCATCAATAGGTAGCAGGATTATTGCAAGTGAAAGCATAAGTGCTTTACGAAAAGATGTTTTATCTAAATGTTAT
GGAGGGGATATTTCTAGGAAAAAGAAACTTTTAAAGAAACAAGCCAAAGGTAAAAAGAGGATGAAGGCAATGGGTAAAGT
TGAAGTCCCTCAAGAAGCTTTTATGGCAGTCTTGAAATTAAACCAGTAA

Upstream 100 bases:

>100_bases
GAAATTAAAAAAAGAAATAATTCCATTTTTGGTTTCCATCCATAATTCTAATTAGCTAGTATTAATCAAGATGTAATAAG
AGGAGAATTTTTCTTTCTAT

Downstream 100 bases:

>100_bases
TATCTTTTAATTTAAAATTTACAGCTATTTATTTTTAAAAGAATTGGGTATATTTCATTTATATCTGTAAAAAAAGATTT
TGAATATTAACTCATTTAAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVQQRDMQEQFLDSMDLERERGITIKLQAARMKYKADDSQEYVLNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADAEKIKQEIEEIIGLDT
SNAINCSAKTGVGIKDILEAIVRRVPPPQDEIKLPTKALIFDSYYDPYRGVIVYFRVISGSLNKREKILLMASKKNYELD
EIGIMAPDQQQVDELHAGEVGYLAASIKSVADARVGDTITLLNSPANDPLPGYKTANPMVFCGLFPTDADQFPDLRVSLE
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNLNQQEHIFIDNPSTIPDPQL
RESIEEPYVKMEIYAPNEFNGTLMGLCQERRGVFIDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRSLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVEVPQEAFMAVLKLNQ

Sequences:

>Translated_602_residues
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVQQRDMQEQFLDSMDLERERGITIKLQAARMKYKADDSQEYVLNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADAEKIKQEIEEIIGLDT
SNAINCSAKTGVGIKDILEAIVRRVPPPQDEIKLPTKALIFDSYYDPYRGVIVYFRVISGSLNKREKILLMASKKNYELD
EIGIMAPDQQQVDELHAGEVGYLAASIKSVADARVGDTITLLNSPANDPLPGYKTANPMVFCGLFPTDADQFPDLRVSLE
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNLNQQEHIFIDNPSTIPDPQL
RESIEEPYVKMEIYAPNEFNGTLMGLCQERRGVFIDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRSLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVEVPQEAFMAVLKLNQ
>Mature_601_residues
TDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVQQRDMQEQFLDSMDLERERGITIKLQAARMKYKADDSQEYVLNLI
DTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADAEKIKQEIEEIIGLDTS
NAINCSAKTGVGIKDILEAIVRRVPPPQDEIKLPTKALIFDSYYDPYRGVIVYFRVISGSLNKREKILLMASKKNYELDE
IGIMAPDQQQVDELHAGEVGYLAASIKSVADARVGDTITLLNSPANDPLPGYKTANPMVFCGLFPTDADQFPDLRVSLEK
LQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKVNLNQQEHIFIDNPSTIPDPQLR
ESIEEPYVKMEIYAPNEFNGTLMGLCQERRGVFIDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIGY
RKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRSLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCYG
GDISRKKKLLKKQAKGKKRMKAMGKVEVPQEAFMAVLKLNQ

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.5996705107084, Blast_Score=622, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=44.8529411764706, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=44.7368421052632, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=44.7368421052632, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=44.7368421052632, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI18390331, Length=151, Percent_Identity=40.3973509933775, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=39.3103448275862, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI217272892, Length=160, Percent_Identity=32.5, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI217272894, Length=160, Percent_Identity=32.5, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI94966752, Length=135, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729339, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI53729337, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=27.843137254902, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=23.768115942029, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI194018520, Length=345, Percent_Identity=23.768115942029, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI194097354, Length=345, Percent_Identity=23.768115942029, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=56.4489112227806, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=478, Percent_Identity=29.4979079497908, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=35.2201257861635, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=155, Percent_Identity=34.8387096774194, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=27.5109170305677, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=619, Percent_Identity=41.8416801292407, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=37.4193548387097, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=144, Percent_Identity=39.5833333333333, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=37.8787878787879, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=37.8787878787879, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=36.1290322580645, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=146, Percent_Identity=35.6164383561644, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=605, Percent_Identity=43.1404958677686, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=41.7808219178082, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=41.7808219178082, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=144, Percent_Identity=42.3611111111111, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=41.2587412587413, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=41.7391304347826, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324761, Length=241, Percent_Identity=28.6307053941909, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322675, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.5182724252492, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=44.5255474452555, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=40.2684563758389, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585709, Length=168, Percent_Identity=36.9047619047619, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=168, Percent_Identity=36.9047619047619, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=168, Percent_Identity=36.9047619047619, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI221458488, Length=153, Percent_Identity=39.8692810457516, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550900, Length=281, Percent_Identity=25.9786476868327, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921738, Length=288, Percent_Identity=28.4722222222222, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28572034, Length=220, Percent_Identity=28.6363636363636, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=236, Percent_Identity=28.3898305084746, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864358, Length=236, Percent_Identity=28.3898305084746, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67556; Mature: 67425

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVQQRDMQEQFLDSMDLERERGITIK
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCEEE
LQAARMKYKADDSQEYVLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEEEEEEECCCCCHHHHEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENNLEIIPVLNKVDLPGADAEKIKQEIEEIIGLDTSNAINCSAKTGVGIKDILEA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHH
IVRRVPPPQDEIKLPTKALIFDSYYDPYRGVIVYFRVISGSLNKREKILLMASKKNYELD
HHHHCCCCCHHCCCCHHEEEECCCCCCHHHHHHEEEHHCCCCCCCEEEEEEECCCCCCHH
EIGIMAPDQQQVDELHAGEVGYLAASIKSVADARVGDTITLLNSPANDPLPGYKTANPMV
HCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPTDADQFPDLRVSLEKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKVNLNQQEHIFIDNPSTIPDPQLRESIEEPYVKMEIYAPNEFN
HHHCCCEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCC
GTLMGLCQERRGVFIDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
CHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEE
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRSLVEKLKELIPKQQFKIPIQASIGS
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCEEEECCCC
RIIASESISALRKDVLSKCYGGDISRKKKLLKKQAKGKKRMKAMGKVEVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
NQ
CC
>Mature Secondary Structure 
TDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVQQRDMQEQFLDSMDLERERGITIK
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCEEE
LQAARMKYKADDSQEYVLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEEEEEEECCCCCHHHHEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENNLEIIPVLNKVDLPGADAEKIKQEIEEIIGLDTSNAINCSAKTGVGIKDILEA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHH
IVRRVPPPQDEIKLPTKALIFDSYYDPYRGVIVYFRVISGSLNKREKILLMASKKNYELD
HHHHCCCCCHHCCCCHHEEEECCCCCCHHHHHHEEEHHCCCCCCCEEEEEEECCCCCCHH
EIGIMAPDQQQVDELHAGEVGYLAASIKSVADARVGDTITLLNSPANDPLPGYKTANPMV
HCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
FCGLFPTDADQFPDLRVSLEKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKVNLNQQEHIFIDNPSTIPDPQLRESIEEPYVKMEIYAPNEFN
HHHCCCEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCC
GTLMGLCQERRGVFIDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
CHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEE
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRSLVEKLKELIPKQQFKIPIQASIGS
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCEEEECCCC
RIIASESISALRKDVLSKCYGGDISRKKKLLKKQAKGKKRMKAMGKVEVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA