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The map label for this gene is pdhC [H]

Identifier: 123967992

GI number: 123967992

Start: 394884

End: 396251

Strand: Direct

Name: pdhC [H]

Synonym: A9601_04551

Alternate gene names: 123967992

Gene position: 394884-396251 (Clockwise)

Preceding gene: 123967991

Following gene: 123967993

Centisome position: 23.65

GC content: 37.57

Gene sequence:

>1368_bases
ATGTCTCACGAAATATTCATGCCTGCCTTGAGTTCTACCATGACGGAGGGCAAGATTGTGGAATGGTTGAAAAATCCAGG
AGATAAAGTTGCAAGAGGTGAATCTGTCTTGGTTGTTGAATCTGACAAGGCAGATATGGATGTTGAATCTTTTCAAGATG
GATACCTTGCAGCTGTTTTAATGCCTGCTGGCAGCACTGCACCAGTAGGAGAGACTATCGGTTTAATTGTAGAAAATGAG
GATGAGATAGCTTCTGTTCAAGAACAAAATAAAGGAAATCAACCCGAAGTTTCTAGTTCGGATCAACTTGAATTGGTAAG
CAATAAAACTGAAGAAAAACCTTTGGTTCAAACTGAAATTGTTGAAAAACAAGAAAAAGAAGTTGTATTAATGAGTGAAA
AGGCAGCCCCATCTTCTAATAGTGATCAAATAAATGCTGCTACGAGTAATGTTTCTTCGAGGGTGATTGCATCTCCAAGA
GCTAAAAAACTTGCCTCTCAAATGGGTGTTGATTTAGCAAAGGTTCATGGATCAGGACCTCATGGAAGGATTCAAGCCGA
TGATATTTTAAAAGCTAATGGCCAACCAGTCTCTATACCATGGATAGGCGAAGGTGGTTCTCCTGCAAGTATCCCTGGTG
TAAATTTGGGGGTTGAAAGTAAACCAGAAGCTTCAGGAAATAGTTTTGGTAATCCCGGAGAAACAGTTCAATTTAATACT
CTTCAAAAAGCGGTAAATAAAAATATGGAATCTAGTTTAGATGTGCCATGTTTTAGAGTGGGTTACTCCATCAACACAGA
TAAATTAGATAATTTTTATAAAAAAGTAAAACAGAATGGAGTGACTATGACTGCTTTACTAGTTAAAGCAGTTGCTAAGA
CACTAAAGAAACACCCTCAAGTTAACTCAAGTTTTTCAGAAAATGGAATTTCTTATCCAGAAAATATAAATATTGCTGTT
GCTGTAGCGATGGAAGATGGTGGACTAATAACTCCAGTTTTAAAAGAACCATGCAATACTGATTTATTTGAATTGTCTAG
GGAATGGAAAGATCTCGTAAAAAGATCAAGATCAAAACAATTAGAACCCGATGAATACTCAACGGGAACCTTCACTTTAT
CTAACCTTGGGATGTTTGGAGTTGATAGATTTGACGCAATTCTTCCTCCAGGTACCGGTGCTATTTTAGCCATAGCATCA
TCGAAACCAACCGTTGTTGCTAATAGTGATGGTTCAATATCTGTTAAAAAAATAATGCAAGTAAATCTAACGGCTGATCA
TAGAGTGATCTATGGAGCTGATGGAGCTTCATTCTTAAAAGACTTGGCTTCCCTAATTCAAGATGAGCCAGAGACTCTTG
TCTCCTAA

Upstream 100 bases:

>100_bases
TTTCGTATAATTAAAACAAGAGTTTAAAGGGTTCTTAATCATAAAAACTTTAGGAAGTTTGTTAGAAATCCCTTGAAACT
TATGCAATAACAATTTTCTT

Downstream 100 bases:

>100_bases
ATTTAATTGATTTCTCAAATTAATAATGAAGAAAGAGATTATAAGCTTGAAGCTTATGATTATTTACTTGATCCATCATT
AATTGCTAGTAAACCTTCTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVARGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASVQEQNKGNQPEVSSSDQLELVSNKTEEKPLVQTEIVEKQEKEVVLMSEKAAPSSNSDQINAATSNVSSRVIASPR
AKKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGGSPASIPGVNLGVESKPEASGNSFGNPGETVQFNT
LQKAVNKNMESSLDVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIQDEPETLVS

Sequences:

>Translated_455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVARGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASVQEQNKGNQPEVSSSDQLELVSNKTEEKPLVQTEIVEKQEKEVVLMSEKAAPSSNSDQINAATSNVSSRVIASPR
AKKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGGSPASIPGVNLGVESKPEASGNSFGNPGETVQFNT
LQKAVNKNMESSLDVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIQDEPETLVS
>Mature_454_residues
SHEIFMPALSSTMTEGKIVEWLKNPGDKVARGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENED
EIASVQEQNKGNQPEVSSSDQLELVSNKTEEKPLVQTEIVEKQEKEVVLMSEKAAPSSNSDQINAATSNVSSRVIASPRA
KKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGGSPASIPGVNLGVESKPEASGNSFGNPGETVQFNTL
QKAVNKNMESSLDVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAVA
VAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASS
KPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIQDEPETLVS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=462, Percent_Identity=28.7878787878788, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=27.6404494382022, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI203098816, Length=172, Percent_Identity=33.7209302325581, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI203098753, Length=172, Percent_Identity=33.7209302325581, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI260898739, Length=150, Percent_Identity=34, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19923748, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786305, Length=311, Percent_Identity=30.5466237942122, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786946, Length=461, Percent_Identity=23.644251626898, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=471, Percent_Identity=29.5116772823779, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=450, Percent_Identity=25.1111111111111, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=29.4117647058824, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25146366, Length=180, Percent_Identity=34.4444444444444, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=469, Percent_Identity=31.9829424307036, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320352, Length=193, Percent_Identity=29.5336787564767, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321632, Length=91, Percent_Identity=41.7582417582418, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582497, Length=312, Percent_Identity=30.4487179487179, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI20129315, Length=312, Percent_Identity=30.4487179487179, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859875, Length=447, Percent_Identity=28.1879194630872, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24645909, Length=198, Percent_Identity=28.7878787878788, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48632; Mature: 48501

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVARGESVLVVESDKADMDVESFQDGYLAAVL
CCCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASVQEQNKGNQPEVSSSDQLELVSNKTEEKPLVQTEI
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHH
VEKQEKEVVLMSEKAAPSSNSDQINAATSNVSSRVIASPRAKKLASQMGVDLAKVHGSGP
HHHHCCCEEEEECCCCCCCCCCCEEHHHHCCCHHHHCCCHHHHHHHHHCCEEEECCCCCC
HGRIQADDILKANGQPVSIPWIGEGGSPASIPGVNLGVESKPEASGNSFGNPGETVQFNT
CCCEEHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LQKAVNKNMESSLDVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQ
HHHHHCCCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANSDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLASLIQDEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SHEIFMPALSSTMTEGKIVEWLKNPGDKVARGESVLVVESDKADMDVESFQDGYLAAVL
CCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASVQEQNKGNQPEVSSSDQLELVSNKTEEKPLVQTEI
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHH
VEKQEKEVVLMSEKAAPSSNSDQINAATSNVSSRVIASPRAKKLASQMGVDLAKVHGSGP
HHHHCCCEEEEECCCCCCCCCCCEEHHHHCCCHHHHCCCHHHHHHHHHCCEEEECCCCCC
HGRIQADDILKANGQPVSIPWIGEGGSPASIPGVNLGVESKPEASGNSFGNPGETVQFNT
CCCEEHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LQKAVNKNMESSLDVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQ
HHHHHCCCCHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANSDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLASLIQDEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA