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The map label for this gene is queA [H]

Identifier: 123967993

GI number: 123967993

Start: 396258

End: 397382

Strand: Direct

Name: queA [H]

Synonym: A9601_04561

Alternate gene names: 123967993

Gene position: 396258-397382 (Clockwise)

Preceding gene: 123967992

Following gene: 123967998

Centisome position: 23.73

GC content: 29.24

Gene sequence:

>1125_bases
TTGATTTCTCAAATTAATAATGAAGAAAGAGATTATAAGCTTGAAGCTTATGATTATTTACTTGATCCATCATTAATTGC
TAGTAAACCTTCTGCAATTAGGCATGCATCAAGATTGATGATAGTGAGAAATAGTTTTTTAGAAGAAGACTGCTTAACTA
ATAAATTTACCAATAATCTTTTAGATGAATTTAGAGAAGGGGATCTTGTAGTTGTAAATAATACTAAAGTTATGAAAGCT
AGGTTAAAAGTTGAATTAGAAAATAAGAAATTAGTCGAATTATTAGTTTTAGAAAGATCCCATGAATGTGTTTGGTTATG
TTTGGCAAAGCCAGCGAAAAAGTTAAAAATAAATAGAAAATTAAAATTAAAATCTCCTTTAGAACAAGAGATTAATTTGA
TTGTTGATGGAGTTGATGAAGAAACTGGAGGGAGATTTATTAAATTTCCGGAAAATATTACTTGTCTCAATTCAATGAAT
GAACTTCTCGATAAATACGGGGAAATTCCTCTCCCTCCTTATATAAAAAATTCCGAAGAAGATTCTTTTCATGAGAAAAG
TTATCAAACTGAGTATGCAACTAATCCTGGGGCAGTTGCTGCACCAACAGCTGGTTTACACTTAAGCAAAAGTCTTATTT
CCAATCTAAAAAAAAAAGGAGTAATAATCTTACCGATAACTTTGCACGTGGGTTATGGAACATTCAAACCAATTGATCAA
GAAGATTTAAGTAACTTAAAACTTCATAAAGAATGGGTAAGTGTTAATAAGGAAGTAGTGAATGAAATAAAAAGAATAAA
GAAAACAGATAGAAAAATAATTGCTATTGGCACAACTAGCGTAAGAGCTCTTGAAAGTTGTTATTCTCACGAAATAAATG
ACTTTATTCCAATAGCTAAATATGTAAATTTAGTAATTAAGCCAGGTTATAAATTTAAGGTAGTTGATGGATTATTAACT
AATTTTCATCTCCCTAAAAGTTCATTATTACTTTTAGTAAGTGCAATGATTGGTAGAGAAAGATTATTAGAATTGTATAA
GAAAGCCATAAAAGAAAAATTTAGATTCTTCTCTTATGGCGATGCGATGTATATTTCACCAGATTCACTACTGGAGAAAA
AATAG

Upstream 100 bases:

>100_bases
TGATCATAGAGTGATCTATGGAGCTGATGGAGCTTCATTCTTAAAAGACTTGGCTTCCCTAATTCAAGATGAGCCAGAGA
CTCTTGTCTCCTAAATTTAA

Downstream 100 bases:

>100_bases
ATTTAGGCTTTGACTGGTTCTTGAATGATTCCGCTTGGAACTTCAGTAAACATAATTGATGATAAATACCTCTCTGCCAA
ATCAGGAAGAACAACTACAA

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MISQINNEERDYKLEAYDYLLDPSLIASKPSAIRHASRLMIVRNSFLEEDCLTNKFTNNLLDEFREGDLVVVNNTKVMKA
RLKVELENKKLVELLVLERSHECVWLCLAKPAKKLKINRKLKLKSPLEQEINLIVDGVDEETGGRFIKFPENITCLNSMN
ELLDKYGEIPLPPYIKNSEEDSFHEKSYQTEYATNPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLSNLKLHKEWVSVNKEVVNEIKRIKKTDRKIIAIGTTSVRALESCYSHEINDFIPIAKYVNLVIKPGYKFKVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLELYKKAIKEKFRFFSYGDAMYISPDSLLEKK

Sequences:

>Translated_374_residues
MISQINNEERDYKLEAYDYLLDPSLIASKPSAIRHASRLMIVRNSFLEEDCLTNKFTNNLLDEFREGDLVVVNNTKVMKA
RLKVELENKKLVELLVLERSHECVWLCLAKPAKKLKINRKLKLKSPLEQEINLIVDGVDEETGGRFIKFPENITCLNSMN
ELLDKYGEIPLPPYIKNSEEDSFHEKSYQTEYATNPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLSNLKLHKEWVSVNKEVVNEIKRIKKTDRKIIAIGTTSVRALESCYSHEINDFIPIAKYVNLVIKPGYKFKVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLELYKKAIKEKFRFFSYGDAMYISPDSLLEKK
>Mature_374_residues
MISQINNEERDYKLEAYDYLLDPSLIASKPSAIRHASRLMIVRNSFLEEDCLTNKFTNNLLDEFREGDLVVVNNTKVMKA
RLKVELENKKLVELLVLERSHECVWLCLAKPAKKLKINRKLKLKSPLEQEINLIVDGVDEETGGRFIKFPENITCLNSMN
ELLDKYGEIPLPPYIKNSEEDSFHEKSYQTEYATNPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLSNLKLHKEWVSVNKEVVNEIKRIKKTDRKIIAIGTTSVRALESCYSHEINDFIPIAKYVNLVIKPGYKFKVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLELYKKAIKEKFRFFSYGDAMYISPDSLLEKK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=365, Percent_Identity=38.0821917808219, Blast_Score=234, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 42756; Mature: 42756

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQINNEERDYKLEAYDYLLDPSLIASKPSAIRHASRLMIVRNSFLEEDCLTNKFTNNL
CCCCCCCCCCCEEEHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEFREGDLVVVNNTKVMKARLKVELENKKLVELLVLERSHECVWLCLAKPAKKLKINRK
HHHHCCCCEEEECCCEEEEEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCE
LKLKSPLEQEINLIVDGVDEETGGRFIKFPENITCLNSMNELLDKYGEIPLPPYIKNSEE
EECCCCHHHHHHEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DSFHEKSYQTEYATNPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
CHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLSNLKLHKEWVSVNKEVVNEIKRIKKTDRKIIAIGTTSVRALESCYSHEINDFIPIAK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCHHHHHHHHH
YVNLVIKPGYKFKVVDGLLTNFHLPKSSLLLLVSAMIGRERLLELYKKAIKEKFRFFSYG
HHHEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECC
DAMYISPDSLLEKK
CEEEECHHHHHCCC
>Mature Secondary Structure
MISQINNEERDYKLEAYDYLLDPSLIASKPSAIRHASRLMIVRNSFLEEDCLTNKFTNNL
CCCCCCCCCCCEEEHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEFREGDLVVVNNTKVMKARLKVELENKKLVELLVLERSHECVWLCLAKPAKKLKINRK
HHHHCCCCEEEECCCEEEEEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCE
LKLKSPLEQEINLIVDGVDEETGGRFIKFPENITCLNSMNELLDKYGEIPLPPYIKNSEE
EECCCCHHHHHHEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
DSFHEKSYQTEYATNPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
CHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLSNLKLHKEWVSVNKEVVNEIKRIKKTDRKIIAIGTTSVRALESCYSHEINDFIPIAK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCHHHHHHHHH
YVNLVIKPGYKFKVVDGLLTNFHLPKSSLLLLVSAMIGRERLLELYKKAIKEKFRFFSYG
HHHEEECCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECC
DAMYISPDSLLEKK
CEEEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA