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The map label for this gene is lipB [H]

Identifier: 123967989

GI number: 123967989

Start: 391562

End: 392212

Strand: Direct

Name: lipB [H]

Synonym: A9601_04521

Alternate gene names: 123967989

Gene position: 391562-392212 (Clockwise)

Preceding gene: 123967984

Following gene: 123967990

Centisome position: 23.45

GC content: 26.42

Gene sequence:

>651_bases
ATGGATAATAGAACAGCAATAATTAAACAACCTGATAATATTTCTTTTTTTAATGATGTTTATAAATTACAAAAAGAATA
TCAAGACGCATTGATTTTAGATAACTCTAACCCTGATTTTATTTGGATAGGGGAGCATCAACTCTGTTATACATTGGGGA
GAGGATCTAATTATGATAATTTACTATTTTCTCTGAATGATGCTAAATATGATGTTTTTAAGATTGATAGAGGTGGTGAG
GTAACTTGTCATATGCCAGGACAATTAGTAACGTATTTGGTTTTAGATTTGAAAAATTTTAATAAAGATTTAAATTGGTA
CTTAAGAAAAATTGAAGAAATTATTATAAAAATCCTTGGAGCTTTTAATATAAATTGTCATTCTAGAGAAGGGTTTACTG
GTGTTTGGATAGGAAATAAGAAAATCGCATCAATTGGAATTGGTTGTAAAAGATGGATTACGATAAATGGATTTTCAATC
AATATTGACTGCGAATTAGAAAACTTTAATAAGATTGTTCCTTGCGGAATAGAAAATTGTCTTATGGCAAATATGATTGA
TTACAATAAAAATTTAAATATTCAAGAAGTCAAGAGAATTGTTAAAAAAATAATTCAGGAAGAATTTAATTTTGATTTTA
TATCACAATAG

Upstream 100 bases:

>100_bases
TATTCTTTTAATGCTCCAGTGAGCTCAAGATTCTTTCCATGGATTAAAATTTTCATAACAAATCTAAAAATATTTACTTA
CTTTCTAAATCTACTTAAAT

Downstream 100 bases:

>100_bases
AAATTAAAATTTCAAAATCAATTTAATATGGGTGATTTAGCATACTGGCCTTCAGCCAAGCCTCTTTCCAAAAAAGATAA
ATTTGCCAAAAAAAGAGATT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDNRTAIIKQPDNISFFNDVYKLQKEYQDALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDAKYDVFKIDRGGE
VTCHMPGQLVTYLVLDLKNFNKDLNWYLRKIEEIIIKILGAFNINCHSREGFTGVWIGNKKIASIGIGCKRWITINGFSI
NIDCELENFNKIVPCGIENCLMANMIDYNKNLNIQEVKRIVKKIIQEEFNFDFISQ

Sequences:

>Translated_216_residues
MDNRTAIIKQPDNISFFNDVYKLQKEYQDALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDAKYDVFKIDRGGE
VTCHMPGQLVTYLVLDLKNFNKDLNWYLRKIEEIIIKILGAFNINCHSREGFTGVWIGNKKIASIGIGCKRWITINGFSI
NIDCELENFNKIVPCGIENCLMANMIDYNKNLNIQEVKRIVKKIIQEEFNFDFISQ
>Mature_216_residues
MDNRTAIIKQPDNISFFNDVYKLQKEYQDALILDNSNPDFIWIGEHQLCYTLGRGSNYDNLLFSLNDAKYDVFKIDRGGE
VTCHMPGQLVTYLVLDLKNFNKDLNWYLRKIEEIIIKILGAFNINCHSREGFTGVWIGNKKIASIGIGCKRWITINGFSI
NIDCELENFNKIVPCGIENCLMANMIDYNKNLNIQEVKRIVKKIIQEEFNFDFISQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=171, Percent_Identity=28.6549707602339, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081767, Length=198, Percent_Identity=28.7878787878788, Blast_Score=104, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=201, Percent_Identity=28.3582089552239, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355483, Length=166, Percent_Identity=31.3253012048193, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25079; Mature: 25079

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRTAIIKQPDNISFFNDVYKLQKEYQDALILDNSNPDFIWIGEHQLCYTLGRGSNYDN
CCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCEEEEEECCCCCCCC
LLFSLNDAKYDVFKIDRGGEVTCHMPGQLVTYLVLDLKNFNKDLNWYLRKIEEIIIKILG
EEEEECCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AFNINCHSREGFTGVWIGNKKIASIGIGCKRWITINGFSINIDCELENFNKIVPCGIENC
HCCCCEECCCCEEEEEECCCEEEEECCCEEEEEEEECEEEEEEEEECCCCEEECCCCHHH
LMANMIDYNKNLNIQEVKRIVKKIIQEEFNFDFISQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDNRTAIIKQPDNISFFNDVYKLQKEYQDALILDNSNPDFIWIGEHQLCYTLGRGSNYDN
CCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCEEEEEECCCCCCCC
LLFSLNDAKYDVFKIDRGGEVTCHMPGQLVTYLVLDLKNFNKDLNWYLRKIEEIIIKILG
EEEEECCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AFNINCHSREGFTGVWIGNKKIASIGIGCKRWITINGFSINIDCELENFNKIVPCGIENC
HCCCCEECCCCEEEEEECCCEEEEECCCEEEEEEEECEEEEEEEEECCCCEEECCCCHHH
LMANMIDYNKNLNIQEVKRIVKKIIQEEFNFDFISQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA