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The map label for this gene is ndhK [H]

Identifier: 123967853

GI number: 123967853

Start: 293052

End: 293786

Strand: Reverse

Name: ndhK [H]

Synonym: A9601_03161

Alternate gene names: 123967853

Gene position: 293786-293052 (Counterclockwise)

Preceding gene: 123967854

Following gene: 123967852

Centisome position: 17.59

GC content: 36.05

Gene sequence:

>735_bases
TTGAAACCACAATTATCCCCAAAAGCAATAAGAGAAATTCGAGAAGGGACTTGCAATCCTCTTGGTGCACCCCAAGTTAC
TACAGATTTGAGCGAAAATATTATATTGACAAGCTTAGACGATCTTCATAATTGGGCTAGACTAAGCAGTTTATGGCCTC
TCTTGTACGGAACAGCTTGTTGTTTTATAGAATTTGCTGCATTAATTGGATCTAGATTTGATTTTGATAGATTTGGATTA
GTACCTAGAAGCTCGCCAAGGCAAGCAGATTTGCTAATAGTTGCAGGAACAGTAACTATGAAGATGGCTCCAGCCCTTGT
TAGGCTTTATGAACAAATGCCTGAACCAAAGTATGTTATCGCTATGGGTGCTTGCACAATCACAGGTGGTATGTTCAGTG
CAGATTCTACAACTGCTGTAAGAGGCGTTGATAAATTGATACCAGTTGATTTATACCTTCCAGGATGTCCACCAAGACCA
GAAGCAATTTTTGATGCTGTAATTAAATTAAGAAAAAAAGTTGGTAATGAATCAATTTTAGAGCGCACAAAAACAGAACA
AACTCACAGATACATAACATCTGATCACGAAATGAATCTCGTTTTCTCAGAAAATACAGGTGAATATCTAAACAAAACTT
CAGCTAACGTAATACCCCCTTCCAAAAAAGAAAAAATAACAGAATTACCTGAAAATACTGAAAAAACTGAAATTATTAAT
TCTGTAGAAGATTAA

Upstream 100 bases:

>100_bases
GGCTTATTAGCTTTTATTGAGGCTTTGATTTTCATTGCAATACTTGTTATTGCCTTAGCATATGCATGGAGAAAAGGTGC
TTTAGAATGGAGTTAAAAAA

Downstream 100 bases:

>100_bases
TGGAAAAAGACAGCCAAGCTACATCCTCAGATACTTCAATAGAAAAAGAAGGGGTTATAAGTCAATCTTTGTCTAAAGAT
GGGATACCAAATCAATCTTT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANVIPPSKKEKITELPENTEKTEIIN
SVED

Sequences:

>Translated_244_residues
MKPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANVIPPSKKEKITELPENTEKTEIIN
SVED
>Mature_244_residues
MKPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANVIPPSKKEKITELPENTEKTEIIN
SVED

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=51.7006802721088, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=48.1707317073171, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=49.6732026143791, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651058, Length=143, Percent_Identity=53.1468531468531, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 27088; Mature: 27088

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANVIPPSKKEKITELPENTEKTEIIN
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
SVED
HCCC
>Mature Secondary Structure
MKPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANVIPPSKKEKITELPENTEKTEIIN
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
SVED
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA