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The map label for this gene is ndhJ [H]

Identifier: 123967852

GI number: 123967852

Start: 292522

End: 293052

Strand: Reverse

Name: ndhJ [H]

Synonym: A9601_03151

Alternate gene names: 123967852

Gene position: 293052-292522 (Counterclockwise)

Preceding gene: 123967853

Following gene: 123967850

Centisome position: 17.55

GC content: 32.02

Gene sequence:

>531_bases
ATGGAAAAAGACAGCCAAGCTACATCCTCAGATACTTCAATAGAAAAAGAAGGGGTTATAAGTCAATCTTTGTCTAAAGA
TGGGATACCAAATCAATCTTTATCTGATGATCATATAGGAATTGAAAACATTTCTGTGCAACCTAGCAAATTATATGAAG
CGGTATCTGCCTTGAGAAATTACGGTTTCAACTATCTCCAATGTCAAGGAGGATATGATGAGGGACCAGGCAAAAATCTT
GTAAGTTTCTACCATTTTATAACTGTTGATGATTTTCAAAAAATTGAAAAAATTAAAGAAGTCAGATTAAAAGTTTTCTT
AAAAAGAGATTCTGATTTATCAATCCCTAGTTTGTACGAAATTTTTAAAGGAAGTGATTGGCAAGAAAGAGAAACTTTTG
ATATGTATGGAATAAATTTTATTGATCATCCCAATCCAAAAAGATTATTAATGCCTGAAGATTGGAGAGGATGGCCATTG
AGAAAAGATTATATTCAGCCAGATTTCTATGAACTTCAGGATGCTTATTAG

Upstream 100 bases:

>100_bases
AAACTTCAGCTAACGTAATACCCCCTTCCAAAAAAGAAAAAATAACAGAATTACCTGAAAATACTGAAAAAACTGAAATT
ATTAATTCTGTAGAAGATTA

Downstream 100 bases:

>100_bases
TTTATTTTTTTTAATTTGCGGTAAATAAACATAACTGCTCTTGCTAGTCTCCTTGGATCATGTCTTAGTGTTGGAGTTAT
TCTTTTTGCATATAGTGGTG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MEKDSQATSSDTSIEKEGVISQSLSKDGIPNQSLSDDHIGIENISVQPSKLYEAVSALRNYGFNYLQCQGGYDEGPGKNL
VSFYHFITVDDFQKIEKIKEVRLKVFLKRDSDLSIPSLYEIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPL
RKDYIQPDFYELQDAY

Sequences:

>Translated_176_residues
MEKDSQATSSDTSIEKEGVISQSLSKDGIPNQSLSDDHIGIENISVQPSKLYEAVSALRNYGFNYLQCQGGYDEGPGKNL
VSFYHFITVDDFQKIEKIKEVRLKVFLKRDSDLSIPSLYEIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPL
RKDYIQPDFYELQDAY
>Mature_176_residues
MEKDSQATSSDTSIEKEGVISQSLSKDGIPNQSLSDDHIGIENISVQPSKLYEAVSALRNYGFNYLQCQGGYDEGPGKNL
VSFYHFITVDDFQKIEKIKEVRLKVFLKRDSDLSIPSLYEIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPL
RKDYIQPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI145693162, Length=89, Percent_Identity=41.5730337078652, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010218
- InterPro:   IPR001268
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa [H]

EC number: 1.6.5.3

Molecular weight: Translated: 20449; Mature: 20449

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDSQATSSDTSIEKEGVISQSLSKDGIPNQSLSDDHIGIENISVQPSKLYEAVSALRN
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YGFNYLQCQGGYDEGPGKNLVSFYHFITVDDFQKIEKIKEVRLKVFLKRDSDLSIPSLYE
CCCCEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
IFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPLRKDYIQPDFYELQDAY
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MEKDSQATSSDTSIEKEGVISQSLSKDGIPNQSLSDDHIGIENISVQPSKLYEAVSALRN
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YGFNYLQCQGGYDEGPGKNLVSFYHFITVDDFQKIEKIKEVRLKVFLKRDSDLSIPSLYE
CCCCEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
IFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPLRKDYIQPDFYELQDAY
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA