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The map label for this gene is 123967840

Identifier: 123967840

GI number: 123967840

Start: 279616

End: 281100

Strand: Reverse

Name: 123967840

Synonym: A9601_03031

Alternate gene names: NA

Gene position: 281100-279616 (Counterclockwise)

Preceding gene: 123967841

Following gene: 123967837

Centisome position: 16.83

GC content: 32.86

Gene sequence:

>1485_bases
GTGACTAATTTACAAGAGAAAAAAATTGATAAATTTAATATTTTTAAAAAAGAAGATTGGTCAAGTGCTTACAAAAATGT
AGAAAAGGAGTTAACTAAAGTGCCTCTAAAAGTTAGCAAAGGTAATAATATTAAAAATTTAAATGGAACATTATTAAGAA
ATGGACCAGGAATATTAGAGAGAGGTGGACAATGGGTTCATCATCCATTTGATGGTGATGGGATGATAACATCCATAAAA
TTCAAAGATGGTCAGCCATTCTTAACAAATAGATTTGTTAAGACTAAAGGCTATTTGGAAGAAGAAAAAATAAATAAATT
TATTTATAGAGGTGTTTTTGGAACACAAAAAAATGGAGGAATTTTAAATAATGCATTAGATCTAAAATTCAAGAATATCG
CTAATACACATGTTATTAAATTAGGAGATGAAATTCTCGCGTTATGGGAAGCAGCAGGTCCACATGCAATGAATCCTGAT
AATCTTGACACTATTGGTTTAACAACATTAAAAGGGGTACTCAAACCTAACGAAGCATTCAGTGCTCATCCCAAAACAGA
CCTAAACTCAAATGCATCTTCAGAACTTTTAGTCACTTTTGGAGTACAAACCGGGCCCAAAAGTACCATTAGATTAATGG
AATTTGATAATGCTGGTACAAATTCTGGAGAGCTCATTTTTGACAGAAAAGATACTTTTAATGGCTTTGCATTTCTTCAT
GATTTTGCAATTACAACTAATTGGGCAATATTTTTACAGAATGCTATTGATTTCAATCCTCTTCCATTTGTAATGGGTCA
AAGAGGAGCAGCACAATGTCTAAAGTCAAACCCAAATAAAAAGGCAAAGTTTTTTATCATCCCAAGAGAAAGTGGATTAT
TTAGAGGACAGCCTCCTTTAACAATAGATGCTCCAGAAGGATTCGTTTTTCATCATGTAAACGCATTTGAAAAAGATTCC
AAAATCGTATTAGATAGTATTTTTTATGATGATTTCCCATCAGTTGGTCCAGATGAGAATTTTAGGGATATTGACTTTGA
TAAATATCCAGAAGGAAAACTGAAAAGATCAATTATCGATCTGAAAACAAAAACTTGTGAACTTGAAACTTTCAGTGAGC
AATGTTGTGAATTTGCTGTTGTTAATCCTAAAAACTTAGGATTAAAAGCAACTTTTAGTTGGATGGCAAGCACATCTCAA
AAGCTGGGGAACGCTCCACTTCAAGCAATAAAAAAAATAAATTTAACTTCTAAGGAAGAGATTTCTTGGTCAGCCGGTCC
AAGTGGATTTGTTAGTGAACCAATTATGGTCCCATCAGAAAACTCTTCAAAAGAAGATGAGGGATTTTTATTTATACTTC
TATGGAACGGAGAAAGAAGAGGAAGCGATTTAGTGATATTAGACGCAAAAGACTTAAAAGAATTAGCTGTTTATGAATTA
CCCATTTCAATTCCTCATGGCCTTCATGGATCTTGGGTCAATTGA

Upstream 100 bases:

>100_bases
AGTAAAGGAATGTTAGAAAAACAATAAAATAGGAATTATTTTCGAATCAGCCACAATTCAGTTGATTTTTGGCGAAGATA
GTTAAAGAGATTATTTTGTT

Downstream 100 bases:

>100_bases
ATTTAAGAAAAAATTTCAATTAAATCTGGAATAATTTTTTTTAATGCTTTACCTCTATGACTACAAGAGTCTTTAATATC
ATTATTCATTTCTGCGAAAG

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTNLQEKKIDKFNIFKKEDWSSAYKNVEKELTKVPLKVSKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIK
FKDGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVIKLGDEILALWEAAGPHAMNPD
NLDTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFDNAGTNSGELIFDRKDTFNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDS
KIVLDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKTKTCELETFSEQCCEFAVVNPKNLGLKATFSWMASTSQ
KLGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
PISIPHGLHGSWVN

Sequences:

>Translated_494_residues
MTNLQEKKIDKFNIFKKEDWSSAYKNVEKELTKVPLKVSKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIK
FKDGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVIKLGDEILALWEAAGPHAMNPD
NLDTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFDNAGTNSGELIFDRKDTFNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDS
KIVLDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKTKTCELETFSEQCCEFAVVNPKNLGLKATFSWMASTSQ
KLGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
PISIPHGLHGSWVN
>Mature_493_residues
TNLQEKKIDKFNIFKKEDWSSAYKNVEKELTKVPLKVSKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIKF
KDGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVIKLGDEILALWEAAGPHAMNPDN
LDTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFDNAGTNSGELIFDRKDTFNGFAFLHD
FAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDSK
IVLDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKTKTCELETFSEQCCEFAVVNPKNLGLKATFSWMASTSQK
LGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYELP
ISIPHGLHGSWVN

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=532, Percent_Identity=22.9323308270677, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4506591, Length=498, Percent_Identity=23.4939759036145, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71998242, Length=508, Percent_Identity=23.2283464566929, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564756, Length=546, Percent_Identity=24.1758241758242, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55171; Mature: 55040

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLQEKKIDKFNIFKKEDWSSAYKNVEKELTKVPLKVSKGNNIKNLNGTLLRNGPGILE
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCCCHHH
RGGQWVHHPFDGDGMITSIKFKDGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGG
CCCCEEECCCCCCCEEEEEEECCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
ILNNALDLKFKNIANTHVIKLGDEILALWEAAGPHAMNPDNLDTIGLTTLKGVLKPNEAF
EECCEEEEEEECCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHCCCCCCC
SAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFDNAGTNSGELIFDRKDTFNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEE
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPL
EHHHHCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSKIVLDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIID
EEECCCCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKTKTCELETFSEQCCEFAVVNPKNLGLKATFSWMASTSQKLGNAPLQAIKKINLTSKEE
HCCCEEEHHHHHHHCCEEEEECCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
ISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
CCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHEEEEE
PISIPHGLHGSWVN
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TNLQEKKIDKFNIFKKEDWSSAYKNVEKELTKVPLKVSKGNNIKNLNGTLLRNGPGILE
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCCCHHH
RGGQWVHHPFDGDGMITSIKFKDGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGG
CCCCEEECCCCCCCEEEEEEECCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
ILNNALDLKFKNIANTHVIKLGDEILALWEAAGPHAMNPDNLDTIGLTTLKGVLKPNEAF
EECCEEEEEEECCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHCCCCCCC
SAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFDNAGTNSGELIFDRKDTFNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEE
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPL
EHHHHCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSKIVLDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIID
EEECCCCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKTKTCELETFSEQCCEFAVVNPKNLGLKATFSWMASTSQKLGNAPLQAIKKINLTSKEE
HCCCEEEHHHHHHHCCEEEEECCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
ISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
CCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHEEEEE
PISIPHGLHGSWVN
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]