Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 123967822

GI number: 123967822

Start: 262631

End: 264091

Strand: Direct

Name: amtB [C]

Synonym: A9601_02851

Alternate gene names: 123967822

Gene position: 262631-264091 (Clockwise)

Preceding gene: 123967821

Following gene: 123967823

Centisome position: 15.73

GC content: 42.98

Gene sequence:

>1461_bases
ATGACCACTGCTTTGCAAACGCCTCAAAGGCGCTCTAGGTCCAGATTACAAGATGCAAGTCTTGTTAATGGACCTATGCT
CCTTTTGAGGAGTATTCGAGGATTTAGTTCAAACCGCTCAATGTTGTGGCTTGCGACAGTTCCTTTAGCTTTGTTTGGTT
TAGGTATTTTTAATCTCTCAGCTCACGCAGCTGATTTACCTGAGTTGAATGCAGCTTTTCTTGCTAACAATTTATGGCTT
TTGATCGCTACTATTTTAGTGATCTTCATGAACGCTGGTTTCGCTATGGTTGAGGCAGGTATGTGTCGTTCTAAGAACGC
AGTAAACATCCTTGCTAAAAACCTATTCGTATTTGCTCTAGCTGTAACCTCTTATTGGTTTATCGGCTACTCATTAATGT
ACGGAGGTAGTGTTGCTGACGGATGGCTTTATTTTGGTGGCTTATTTTTTGATCCAACAGTTACTGCAGATATGGTAACT
GATGCTGGATTAGTCCCAACAGTTGATTTCTTGTTCCAGTCTGCATTCGCAGGAACTGCGGCAACTATCGTATCCGGTCT
TGTTGCTGAAAGAGTTAAATTTGGAGAATTTGTTGTTTTTGCTGTTGTATTAACTGCATTTATATATCCAATTGCTGGTA
GCTGGAAATGGAATGGTGGTTGGCTTGATTCTTTGGGTTTTGTAGACTTTGCCGGTTCTTCAATTGTTCACTCAGTTGGA
GCATGGGCTGGTCTTGTAGGAGCTATGCTTCTTGGACCAAGAATTGGCAAATACTCTGATGGAAAACCACAGGCTATGCC
AGGACACAATATGGCTATAGCTACTCTAGGTGCATTAGTTCTATGGATAGGTTGGTATGGTTTTAACCCCGGTTCTCAAC
TTGCTATGGATCAATGGGTTCCATATGTTGCTGTAACAACTACTTTGGCAGCAGCAGCTGGTGCTATTGGTGCAACTATT
GTTTCAACATTAACTTCTGGTAAGCCTGATCTTACAATGATAATTAACGGAATCCTTGCTGGTTTGGTTAGTATCACTGC
TGGTTGTGGTGATATGACTCTTGCTGGAGCCTGGTTCGCAGGACTAGTGGGAGGAATAATCGTTGTATTTTCTGTTGCAG
CACTTGATGCCGCTGAGATTGATGATCCTGTAGGCGCATTCTCTGTTCACGGAGTTTGTGGTGTATGGGGTACTGTTGTT
ATCGGTCTTTGGGGTACAGCTGTACAAGGTGACGGAGCAGGTATGGGATTGTTCAATGGCGGAGGTATTACCCTTCTTCT
AGTTCAAGCTCTTGGTGCCGCAGCTTATGCTATTTGGACACTAGTTACTTGCTGGATTGCCTGGTCTGTAATTGGAGGAT
TATTCGGAGGAATCCGTGTATCTGAAGAGGAAGAGACTCAAGGTTTAGATATAGGAGAGCATGGAATGGAAGCATATCCA
GACTTTGCATCTGCTAAATAA

Upstream 100 bases:

>100_bases
TTGGTAACAACGACTACTAGACACTAATAAGTTTTTTGAGCAAAATTAAATATGAAAGGAAACAGCACAAACTGTTTTTT
TGCAGATCTAATTTTTTTTT

Downstream 100 bases:

>100_bases
TCTAACTAAAAATTAATTTAAGAAACCTGACTTATGTCAGGTTTCTTTTTTTTACGAAAAATTATTTAAAACGTTGTAAT
ATAGGAAAATGGATACTCAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTALQTPQRRSRSRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGIFNLSAHAADLPELNAAFLANNLWL
LIATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGYSLMYGGSVADGWLYFGGLFFDPTVTADMVT
DAGLVPTVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFAVVLTAFIYPIAGSWKWNGGWLDSLGFVDFAGSSIVHSVG
AWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGATI
VSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLAGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTVV
IGLWGTAVQGDGAGMGLFNGGGITLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAYP
DFASAK

Sequences:

>Translated_486_residues
MTTALQTPQRRSRSRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGIFNLSAHAADLPELNAAFLANNLWL
LIATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGYSLMYGGSVADGWLYFGGLFFDPTVTADMVT
DAGLVPTVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFAVVLTAFIYPIAGSWKWNGGWLDSLGFVDFAGSSIVHSVG
AWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGATI
VSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLAGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTVV
IGLWGTAVQGDGAGMGLFNGGGITLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAYP
DFASAK
>Mature_485_residues
TTALQTPQRRSRSRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGIFNLSAHAADLPELNAAFLANNLWLL
IATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFALAVTSYWFIGYSLMYGGSVADGWLYFGGLFFDPTVTADMVTD
AGLVPTVDFLFQSAFAGTAATIVSGLVAERVKFGEFVVFAVVLTAFIYPIAGSWKWNGGWLDSLGFVDFAGSSIVHSVGA
WAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWVPYVAVTTTLAAAAGAIGATIV
STLTSGKPDLTMIINGILAGLVSITAGCGDMTLAGAWFAGLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTVVI
GLWGTAVQGDGAGMGLFNGGGITLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAYPD
FASAK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=35.4368932038835, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32564042, Length=453, Percent_Identity=32.6710816777042, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI133931100, Length=426, Percent_Identity=33.3333333333333, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17550180, Length=449, Percent_Identity=34.5211581291759, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=33.4106728538283, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=29.6037296037296, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=422, Percent_Identity=27.4881516587678, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=420, Percent_Identity=28.0952380952381, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078314, Length=425, Percent_Identity=36.7058823529412, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50714; Mature: 50583

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALQTPQRRSRSRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGIFNLS
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCC
AHAADLPELNAAFLANNLWLLIATILVIFMNAGFAMVEAGMCRSKNAVNILAKNLFVFAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHH
AVTSYWFIGYSLMYGGSVADGWLYFGGLFFDPTVTADMVTDAGLVPTVDFLFQSAFAGTA
HHHHHHHHHHHHHHCCCCCCCHHHHCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
ATIVSGLVAERVKFGEFVVFAVVLTAFIYPIAGSWKWNGGWLDSLGFVDFAGSSIVHSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHH
AWAGLVGAMLLGPRIGKYSDGKPQAMPGHNMAIATLGALVLWIGWYGFNPGSQLAMDQWV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
PYVAVTTTLAAAAGAIGATIVSTLTSGKPDLTMIINGILAGLVSITAGCGDMTLAGAWFA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GLVGGIIVVFSVAALDAAEIDDPVGAFSVHGVCGVWGTVVIGLWGTAVQGDGAGMGLFNG
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEECC
GGITLLLVQALGAAAYAIWTLVTCWIAWSVIGGLFGGIRVSEEEETQGLDIGEHGMEAYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHCC
DFASAK
CCCCCC
>Mature Secondary Structure 
TTALQTPQRRSRSRLQDASLVNGPMLLLRSIRGFSSNRSMLWLATVPLALFGLGIFNLS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCC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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]