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The map label for this gene is trmB

Identifier: 123967799

GI number: 123967799

Start: 244138

End: 244767

Strand: Direct

Name: trmB

Synonym: A9601_02621

Alternate gene names: 123967799

Gene position: 244138-244767 (Clockwise)

Preceding gene: 123967796

Following gene: 123967801

Centisome position: 14.62

GC content: 24.29

Gene sequence:

>630_bases
ATGAGACAGCATGTTAATCCGCTTAGTATTAATTTCAATCAAATTGAGAGAATACCATCTTTGGGTGAAATGTTTGGTGA
TTCTAAATTAAATCTTCATTTGGATATAGGCTGTGCTGCTGGAGAGTTTTTATTCGATTTAGCTTTAGTAAATACTAGTT
GGAATTATTTAGGAATCGAAATCCGTGAGAAATTAGTCAAAAATGCTAAATTAAAAGTACTTGAAAGTGAAATCAAAAAT
TTATATTTTTTATTTGGTAATGCTAATAATATTTTGAATGATGTCCAAAGTGAATTGATTATTAAAAATTTAAAAAGTAT
TTCTTTTTATTTTCCTGATCCATGGTTTAAAAAGAGACATTATAAAAGGCGTGTCATTCAGCCAGAATTTATTAATATTC
TCTCTAATTTATTGCAAAAAGGTACCTTAATTTTTATAAAAACAGATGTAAAGGATTTATTTGATTATATGGATTACACT
ATTTCTAATAATTTTTATTTTAAAACGATAGATAAAAAAGATTTTAATTATTCTGAAAGTTTTAATCCAAATAAAGTGAA
AACTAATCGGGAAAAGTATGTGATTAATAATCAACTTGATATTTTTGAAAGGATTTATATAAAAATTTGA

Upstream 100 bases:

>100_bases
AATAATTAAACATCAAACTGTTACTTTTGAACTCACTATTGTCATAGTTATGAATATTATGCATCCTTATATTATTGCCT
AAAAATATATTAATAATCGT

Downstream 100 bases:

>100_bases
TTCATTTTTAATTTATTATTTTATTAATTTCTAAACTGAGTTTGTTTGTTATTTCGCTTGATAAAGAATTAACTTTCTTA
TGACTTTTGGCCTCAACAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MRQHVNPLSINFNQIERIPSLGEMFGDSKLNLHLDIGCAAGEFLFDLALVNTSWNYLGIEIREKLVKNAKLKVLESEIKN
LYFLFGNANNILNDVQSELIIKNLKSISFYFPDPWFKKRHYKRRVIQPEFINILSNLLQKGTLIFIKTDVKDLFDYMDYT
ISNNFYFKTIDKKDFNYSESFNPNKVKTNREKYVINNQLDIFERIYIKI

Sequences:

>Translated_209_residues
MRQHVNPLSINFNQIERIPSLGEMFGDSKLNLHLDIGCAAGEFLFDLALVNTSWNYLGIEIREKLVKNAKLKVLESEIKN
LYFLFGNANNILNDVQSELIIKNLKSISFYFPDPWFKKRHYKRRVIQPEFINILSNLLQKGTLIFIKTDVKDLFDYMDYT
ISNNFYFKTIDKKDFNYSESFNPNKVKTNREKYVINNQLDIFERIYIKI
>Mature_209_residues
MRQHVNPLSINFNQIERIPSLGEMFGDSKLNLHLDIGCAAGEFLFDLALVNTSWNYLGIEIREKLVKNAKLKVLESEIKN
LYFLFGNANNILNDVQSELIIKNLKSISFYFPDPWFKKRHYKRRVIQPEFINILSNLLQKGTLIFIKTDVKDLFDYMDYT
ISNNFYFKTIDKKDFNYSESFNPNKVKTNREKYVINNQLDIFERIYIKI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=174, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320000, Length=147, Percent_Identity=27.891156462585, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PROMS (A2BP39)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001008657.1
- ProteinModelPortal:   A2BP39
- SMR:   A2BP39
- STRING:   A2BP39
- GeneID:   4716947
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_02621
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   PDPWWKR
- PhylomeDB:   A2BP39
- ProtClustDB:   PRK00121
- BioCyc:   PMAR146891:A9601_02621-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24784; Mature: 24784

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: BINDING 35-35 BINDING 60-60 BINDING 87-87 BINDING 113-113 BINDING 117-117 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHVNPLSINFNQIERIPSLGEMFGDSKLNLHLDIGCAAGEFLFDLALVNTSWNYLGIE
CCCCCCCEEECHHHHHHCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEHHH
IREKLVKNAKLKVLESEIKNLYFLFGNANNILNDVQSELIIKNLKSISFYFPDPWFKKRH
HHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHH
YKRRVIQPEFINILSNLLQKGTLIFIKTDVKDLFDYMDYTISNNFYFKTIDKKDFNYSES
HHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
FNPNKVKTNREKYVINNQLDIFERIYIKI
CCCCCCCCCCCEEEECCHHHHHHEEEECC
>Mature Secondary Structure
MRQHVNPLSINFNQIERIPSLGEMFGDSKLNLHLDIGCAAGEFLFDLALVNTSWNYLGIE
CCCCCCCEEECHHHHHHCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEHHH
IREKLVKNAKLKVLESEIKNLYFLFGNANNILNDVQSELIIKNLKSISFYFPDPWFKKRH
HHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHH
YKRRVIQPEFINILSNLLQKGTLIFIKTDVKDLFDYMDYTISNNFYFKTIDKKDFNYSES
HHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
FNPNKVKTNREKYVINNQLDIFERIYIKI
CCCCCCCCCCCEEEECCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA