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The map label for this gene is prfB [H]

Identifier: 123967735

GI number: 123967735

Start: 183362

End: 184426

Strand: Direct

Name: prfB [H]

Synonym: A9601_01981

Alternate gene names: 123967735

Gene position: 183362-184426 (Clockwise)

Preceding gene: 123967732

Following gene: 123967736

Centisome position: 10.98

GC content: 34.84

Gene sequence:

>1065_bases
ATGCTCAGGATTGTCTTTGACGTTCCAAGATTAAAAGCGAAAAGGAAAGAGTTAGAGCAAATTTCTGCTCAGCCTGAATT
TTGGGATAATCAAGAAGAAGCTAAAAAGCAAATGTTAATCCTTGATGATGTCAAAGCACAACTCGAATTGTTAGATAAAT
GGAAAACTTTTATTTCTGATGCTAATGCCTCTCTTGAGCTTTATTCTCTAGAACCCGAAGAGGAAATGATTTTAGAATCG
CAGCTGGGATTAAAGAAATTAAGAGAGAATTTGGATAAATGGGAATTTGAGAGATTGTTATGTGGTGAATACGATAAGGA
AGGAGCAGTAGTTTCTATTAACGCAGGTGCGGGAGGAACAGATGCGCAGGATTGGGTAGAGATATTATTAAGAATGTACT
CAAGATGGGCCGATAATAATCAAATGAGCCTTGTCATTAATGAATTATCTCAAGGAGAAGAAGCTGGTATTAAAAGTGTA
ACGTTCGAAATTGAAGGAAAATATGCATATGGCTATCTGCAACATGAAAAAGGAACTCATAGATTAGTAAGAATTTCTCC
TTTTAATGCCAATGGCAAAAGACAAACCAGCTTTGCTGGGGTAGAGGTTATGCCAAAATTAGATGATAATATTTCACTTG
ATATACCTGAAAAAGATTTAGAAATTACAACGAGTAGATCCGGTGGAGCTGGAGGACAAAATGTTAATAAAGTAGAAACA
GCAGTGAGAATTGTTCATTTGCCTTCAGGAATTTCAGTAAGATGTACTCAAGAAAGATCTCAATTACAAAATAAAGAAAA
AGCTATGTTACTTTTAAAGTCTAAACTATTAGTGATTGCTAAAGAACAACGAGCTGCTGAAGTCGCTGATATTAAAGGTG
ATATTGTGGAAGCTGCATGGGGCAATCAAATAAGAAATTATGTTTTCCATCCCTATCAAATGGTAAAAGATCTTAGGACT
ATGCAGGAGACTAACGAGTTAGATAGTGTTTTAGATGGTGGACTTGACCCATTTATTCATGAATTATTACGTATGAATAT
TTCTTCTAATGAATCTATTGAATAA

Upstream 100 bases:

>100_bases
CAAAAGTGCTTGTTAGATTATAAATAACGATTAAATTCTATTTTGATAGATATTACAGATTTCAAAAAAGATCTTTCGGA
ACTAACAGAGCGCCTGGGTA

Downstream 100 bases:

>100_bases
CTAATGGAAAATAAAAACGAAATTTTAGAAAAAAAAGTTTCTCCTCCAAGCTTTATCAAGCTTGCTATGAGAAATATGGT
AAGGAAAGGCTCAAAAAGTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLRIVFDVPRLKAKRKELEQISAQPEFWDNQEEAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QLGLKKLRENLDKWEFERLLCGEYDKEGAVVSINAGAGGTDAQDWVEILLRMYSRWADNNQMSLVINELSQGEEAGIKSV
TFEIEGKYAYGYLQHEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEVADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLDPFIHELLRMNISSNESIE

Sequences:

>Translated_354_residues
MLRIVFDVPRLKAKRKELEQISAQPEFWDNQEEAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QLGLKKLRENLDKWEFERLLCGEYDKEGAVVSINAGAGGTDAQDWVEILLRMYSRWADNNQMSLVINELSQGEEAGIKSV
TFEIEGKYAYGYLQHEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEVADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLDPFIHELLRMNISSNESIE
>Mature_354_residues
MLRIVFDVPRLKAKRKELEQISAQPEFWDNQEEAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QLGLKKLRENLDKWEFERLLCGEYDKEGAVVSINAGAGGTDAQDWVEILLRMYSRWADNNQMSLVINELSQGEEAGIKSV
TFEIEGKYAYGYLQHEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEVADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLDPFIHELLRMNISSNESIE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=248, Percent_Identity=34.2741935483871, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI34577120, Length=251, Percent_Identity=33.0677290836653, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=34.6938775510204, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=42.8994082840237, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=38.0165289256198, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542784, Length=231, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321295, Length=234, Percent_Identity=30.7692307692308, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921226, Length=210, Percent_Identity=37.1428571428571, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40185; Mature: 40185

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIVFDVPRLKAKRKELEQISAQPEFWDNQEEAKKQMLILDDVKAQLELLDKWKTFISD
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHCC
ANASLELYSLEPEEEMILESQLGLKKLRENLDKWEFERLLCGEYDKEGAVVSINAGAGGT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
DAQDWVEILLRMYSRWADNNQMSLVINELSQGEEAGIKSVTFEIEGKYAYGYLQHEKGTH
CHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCCEEEEEEEECEEEEEEEECCCCCC
RLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHH
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEVADIKGDIVEAAW
EEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHH
GNQIRNYVFHPYQMVKDLRTMQETNELDSVLDGGLDPFIHELLRMNISSNESIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLRIVFDVPRLKAKRKELEQISAQPEFWDNQEEAKKQMLILDDVKAQLELLDKWKTFISD
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHCC
ANASLELYSLEPEEEMILESQLGLKKLRENLDKWEFERLLCGEYDKEGAVVSINAGAGGT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
DAQDWVEILLRMYSRWADNNQMSLVINELSQGEEAGIKSVTFEIEGKYAYGYLQHEKGTH
CHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCCEEEEEEEECEEEEEEEECCCCCC
RLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHH
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEVADIKGDIVEAAW
EEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHH
GNQIRNYVFHPYQMVKDLRTMQETNELDSVLDGGLDPFIHELLRMNISSNESIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]