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The map label for this gene is ndhF [H]

Identifier: 123967703

GI number: 123967703

Start: 154504

End: 156516

Strand: Direct

Name: ndhF [H]

Synonym: A9601_01661

Alternate gene names: 123967703

Gene position: 154504-156516 (Clockwise)

Preceding gene: 123967702

Following gene: 123967704

Centisome position: 9.25

GC content: 36.02

Gene sequence:

>2013_bases
ATGCCACAAGCTTCAGAAATTGCCTGGTTAATCCCCGTTTTTCCACTAATTGGAGCAGTGCTTTCTGGCTTAGGACTAAT
AAGCATTAACAAGAAAATTAATAATTCAAGAGAAATAGTTTCTGTAGGTCTGATTTCTTTCGTTGGGATTTCTGCGGTAA
TTAGTTATAAAGCTTTAATTGAACAAGTTAATGGTTATCAATCAGTAGAAAAATTATTTGTATGGGCCAATGCAGGGGAT
TTTACAATTCCAATGGGATTTGTCCTTGATCCTTTGGGGAGTGTAATGCTTGCGTTAGTAACTACAATAACTTTGCTGGT
AATGATTTACTCTCATGGTTACATGGCGCATGACAAAGGATATGTCAGATTTTTTACATATTTAGCATTATTTAGTAGTT
CAATGATGGGATTGATAGTTAGTCCGAATTTATTAGAAATTTATGTTTTTTGGGAATTGGTTGGGATGTGTTCTTACTTA
TTAGTTGGTTTTTGGTACGACAGGGATGGCGCTGCACACGCTGCACAAAAAGCATTTGTTGTTAATAGAGTGGGAGATTT
TGGTTTATTACTAGGAATTCTTGGCCTATTTTGGGCTACAAATAGTTTTGATTTTAATGAAATAGCTACTGGAATTTCTC
AATCGATATCTGACCATTCGATACCTATTTGGGCTGCTTTATTACTTTGTTTTTTAGTTTTTTTAGGGCCAATGGCTAAA
TCCGCTCAGTTTCCTCTTCATGTGTGGTTGCCTGATGCGATGGAAGGTCCTACACCCATTTCTGCACTTATCCATGCTGC
AACAATGGTTGCAGCAGGAATCTTTCTTGTAGCAAGACTTCAACCTTTGTATTCAATATTCCCCTCTATTCAGTTCATTA
TTGCTTTAGTTGGTACCATTACTTGTTTTTTAGGAGCCTCTATAGCTTTGACACAAATGGATTTAAAAAAAGGTTTAGCA
TATAGCACAGTTTCTCAGCTTGGGTATATGATGCTCGCAATGGGTTGTGGAGCACCAGTTGCAGGAATTTTTCATTTAGT
AACTCATGCTTGCTTTAAAGCAATGCTATTTTTGGGATCTGGTTCAGTAATACATGCTATGGAAGAAGTAGTTGGTCATC
AGCCTGTATTAGCTCAAGATATGAGATTAATGGGCGGTTTAAGAAAAAAAATGCCATACACATCAACAACATTTTTAATA
GGTTGTGTAGCAATTAGTGGAATTCCCCCACTGGCAGGTTTTTGGAGTAAAGACGAGATACTCGGAAATGCTTTTATATC
ATTTCCAGCTTTTTGGTTCGTAGGACTTTTAACAGCTGGTATGACTGCTTTTTATATGTTTAGGCTTTATTTCTTGACAT
TTGAAGGAGATTTCAGAGGGGATAATAAAGAATTGCAAAAACAGCTTCTAATAGCCTCTAAAACAAACCTAGATGAAGAA
AATGAAGAAGAGCATGAAGAACATGGCTCTATCCATGAGTCACCCTGGTCAATGACATTTCCCTTAGTATTTCTAGCTGT
ACCGTCGGTAATAATTGGTTTTATGGGACTTCCATGGGATAGCAAAATTGCAAATTTACTAGATCCTGAAGAAGCAGAGA
CTGCTGCAAAAGCCTTCGAATTTAAAGAATTTTTGCCTTTAGCAATTGCCTCGGTACTTATCGCATCAGCTGGCATCATT
ATTGCTTATCAGGCATATTTTGTGAAAAAAATTAATTTATCAGTTTTATTTGCCGAAAAGTTTCCTAGCATTAATCGATT
TTTATCCAACAAATGGTATCTAGATGATATTAATGAAAAACTTTTTGTTAAGGGTAGTAGGAAACTTGCTAAAGAAGTTT
TAGAGGTTGATTCTAAGGTTGTTGATGGCGTCGTTAATCTTACTGGACTTGTAACTTTAGGAAGTGGAGAAGGTTTAAAA
TATTTTGAGACTGGTAGGGCTCAATTTTATGCGCTTATTGTTTTTGGAGGAGTCATTTTACTAGTTGCTATATTTGGTTT
TCAATCTCCTTAA

Upstream 100 bases:

>100_bases
TCATCAATATATAAATATTGCTGTTAAAACATTTAATTCATCATTTTTGTCTGAATTTTTCAATTGACAGTTTAAAATCA
AAATATAAATTCTTTAAAAA

Downstream 100 bases:

>100_bases
GTATCATAATTACAATTGTGTGTCTTCACTGTCCATTGGGGTGAAAAAATACAGAAAATTTCTAGACTTTATTTAAGATA
AATTTCTTATTAAATTGAGT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWANAGD
FTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIVSPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIATGISQSISDHSIPIWAALLLCFLVFLGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPSIQFIIALVGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPVLAQDMRLMGGLRKKMPYTSTTFLI
GCVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGDNKELQKQLLIASKTNLDEE
NEEEHEEHGSIHESPWSMTFPLVFLAVPSVIIGFMGLPWDSKIANLLDPEEAETAAKAFEFKEFLPLAIASVLIASAGII
IAYQAYFVKKINLSVLFAEKFPSINRFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLK
YFETGRAQFYALIVFGGVILLVAIFGFQSP

Sequences:

>Translated_670_residues
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWANAGD
FTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIVSPNLLEIYVFWELVGMCSYL
LVGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIATGISQSISDHSIPIWAALLLCFLVFLGPMAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPSIQFIIALVGTITCFLGASIALTQMDLKKGLA
YSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPVLAQDMRLMGGLRKKMPYTSTTFLI
GCVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGDNKELQKQLLIASKTNLDEE
NEEEHEEHGSIHESPWSMTFPLVFLAVPSVIIGFMGLPWDSKIANLLDPEEAETAAKAFEFKEFLPLAIASVLIASAGII
IAYQAYFVKKINLSVLFAEKFPSINRFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLK
YFETGRAQFYALIVFGGVILLVAIFGFQSP
>Mature_669_residues
PQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALIEQVNGYQSVEKLFVWANAGDF
TIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKGYVRFFTYLALFSSSMMGLIVSPNLLEIYVFWELVGMCSYLL
VGFWYDRDGAAHAAQKAFVVNRVGDFGLLLGILGLFWATNSFDFNEIATGISQSISDHSIPIWAALLLCFLVFLGPMAKS
AQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPSIQFIIALVGTITCFLGASIALTQMDLKKGLAY
STVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGSGSVIHAMEEVVGHQPVLAQDMRLMGGLRKKMPYTSTTFLIG
CVAISGIPPLAGFWSKDEILGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGDNKELQKQLLIASKTNLDEEN
EEEHEEHGSIHESPWSMTFPLVFLAVPSVIIGFMGLPWDSKIANLLDPEEAETAAKAFEFKEFLPLAIASVLIASAGIII
AYQAYFVKKINLSVLFAEKFPSINRFLSNKWYLDDINEKLFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKY
FETGRAQFYALIVFGGVILLVAIFGFQSP

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=461, Percent_Identity=36.4425162689805, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1788614, Length=648, Percent_Identity=37.037037037037, Blast_Score=331, Evalue=9e-92,
Organism=Escherichia coli, GI1788829, Length=403, Percent_Identity=30.272952853598, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1788831, Length=361, Percent_Identity=30.7479224376731, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788827, Length=429, Percent_Identity=27.7389277389277, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788613, Length=369, Percent_Identity=26.8292682926829, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI2367154, Length=402, Percent_Identity=26.1194029850746, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 73322; Mature: 73191

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQVNGYQSVEKLFVWANAGDFTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKG
HHHCCHHHHHHEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMMGLIVSPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATNSFDFNEIATGISQSISDHSIPIWAALLLCFLVFLGPMAK
HHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPSIQFIIALVGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHQPVLAQDMRLMGGLRKKMPYTSTTFLIGCVAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGDNKELQKQLLIASKTNLDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCC
NEEEHEEHGSIHESPWSMTFPLVFLAVPSVIIGFMGLPWDSKIANLLDPEEAETAAKAFE
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHH
FKEFLPLAIASVLIASAGIIIAYQAYFVKKINLSVLFAEKFPSINRFLSNKWYLDDINEK
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHEECCEEEEHHHCCHHHHHHCCCCEEECCCCC
LFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKYFETGRAQFYALIVFGGVIL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHH
LVAIFGFQSP
HHHHHHCCCC
>Mature Secondary Structure 
PQASEIAWLIPVFPLIGAVLSGLGLISINKKINNSREIVSVGLISFVGISAVISYKALI
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQVNGYQSVEKLFVWANAGDFTIPMGFVLDPLGSVMLALVTTITLLVMIYSHGYMAHDKG
HHHCCHHHHHHEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
YVRFFTYLALFSSSMMGLIVSPNLLEIYVFWELVGMCSYLLVGFWYDRDGAAHAAQKAFV
HHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
VNRVGDFGLLLGILGLFWATNSFDFNEIATGISQSISDHSIPIWAALLLCFLVFLGPMAK
HHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGIFLVARLQPLYSIFPSIQFIIALVGTI
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCFLGASIALTQMDLKKGLAYSTVSQLGYMMLAMGCGAPVAGIFHLVTHACFKAMLFLGS
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GSVIHAMEEVVGHQPVLAQDMRLMGGLRKKMPYTSTTFLIGCVAISGIPPLAGFWSKDEI
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LGNAFISFPAFWFVGLLTAGMTAFYMFRLYFLTFEGDFRGDNKELQKQLLIASKTNLDEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCC
NEEEHEEHGSIHESPWSMTFPLVFLAVPSVIIGFMGLPWDSKIANLLDPEEAETAAKAFE
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHH
FKEFLPLAIASVLIASAGIIIAYQAYFVKKINLSVLFAEKFPSINRFLSNKWYLDDINEK
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHEECCEEEEHHHCCHHHHHHCCCCEEECCCCC
LFVKGSRKLAKEVLEVDSKVVDGVVNLTGLVTLGSGEGLKYFETGRAQFYALIVFGGVIL
EEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHH
LVAIFGFQSP
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]