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The map label for this gene is cca [H]

Identifier: 123967695

GI number: 123967695

Start: 147607

End: 148824

Strand: Direct

Name: cca [H]

Synonym: A9601_01581

Alternate gene names: 123967695

Gene position: 147607-148824 (Clockwise)

Preceding gene: 123967692

Following gene: 123967696

Centisome position: 8.84

GC content: 26.44

Gene sequence:

>1218_bases
ATGAAAAAGAGCTTTCACACAGATCATACACTAATAATTGATGAATTAGAAACAAGTATAAAAGTTCATAATTTGGATTT
AATCTTAGGTTTTTTACCTACAGGATCATATTTGGTTGGTGGTTATATAAGAGATATTATTTTGGGAAGAAAAACTGAAA
AGTTGGATGTTGATATTGTGGTACCTTTAAATGCAATTGAAATTGGTAAAAAGATTGCAGATAATATTAGATCAAAATTA
ATAATTTTAGATAAAAAAAGAGAAGTAGTAAGAATTATTCTTAACAATATTTATATTGATATTGCTAATCAAGTTTCATC
TTCCATAGAAGGAGATTTATGTTCTAGAGATTTGTCGATTAATTCAATTGCTTTTTTATTTGATAAGAAGAGTTTGTTTG
ATCCATTAAATGGTCTTAAAGATCTTGAGATTTCTTTGCTTAGAACTCATTCTGAAAACAATTTATTAAATGATCCTTTA
CGAATTTTAAGATGTTTTCGATTTGTTTCAGAATTGAATTTTAAAATTGATTCAAGATTACTTGATTTTATAAAAAAAAA
TAAAAGGAACTTACATCTTGTCGCAAAAGAGAGAATTACTTATGAAATACAGAAAATAGTAAATGGTGCAAATGCTCTTG
ATGCAGTATTGTTGATAAAAAAATTAAATATATTTGATACTGAAAATTTATTTGAAGATTCTTTTTATTTGGATTTAGAG
AAAATTAATTATGGAGAACTTGATCAGGAAGAACAAGATAAATATTTACCATCATTTTTTATTGCTCAAATCTTAGATGT
TGTAACTTTAGAGAAACTGCAATTTAGTAAAGCTGATATTGCAAAAAATAAGTTATTGCGAAAATGGCACCTTCTCTTGA
AAAACAAAAATATCGCTCAGTTAGATGAATTTGACAGATTTAAATTACATCAAGAATTAGAAATGTTTCTGCCATCTTTT
ATTTTTTATTTACCTGAAAACTTACAATTAGATTGGCTTCATAGATGGCGTGACAACGATGATAAATTATTTCATCCCTC
AAACATAGTTAATGGTGATGTAATTAAAAAAAATTTGGAAATCAAGGATGGACCTATCTTGGGAGAGCTTTTACAGTATC
TTTCTAAGGAACTTGCATATAAGAGATTGAATAATTTTGATGAAGCTATTTATAAAGCAAAGCGATGGATTGAACAAAAT
GCGCCAAAATGTGATTAA

Upstream 100 bases:

>100_bases
CAAATATGATCAACACCATGATTATTTTCAACATCATGATATGTGATACATCTATCAACCCATTTACAGTTGATGCAAAT
GCACATAATTAATAAATAAA

Downstream 100 bases:

>100_bases
ATACACATAGCTTAGTTTTGTTTAGAAGTTCAGACTTCAAAATCATTTTTAAAAATTTATGAGTATTCGCATTTACATTG
GTAACTTACCACAAGGATTT

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKKSFHTDHTLIIDELETSIKVHNLDLILGFLPTGSYLVGGYIRDIILGRKTEKLDVDIVVPLNAIEIGKKIADNIRSKL
IILDKKREVVRIILNNIYIDIANQVSSSIEGDLCSRDLSINSIAFLFDKKSLFDPLNGLKDLEISLLRTHSENNLLNDPL
RILRCFRFVSELNFKIDSRLLDFIKKNKRNLHLVAKERITYEIQKIVNGANALDAVLLIKKLNIFDTENLFEDSFYLDLE
KINYGELDQEEQDKYLPSFFIAQILDVVTLEKLQFSKADIAKNKLLRKWHLLLKNKNIAQLDEFDRFKLHQELEMFLPSF
IFYLPENLQLDWLHRWRDNDDKLFHPSNIVNGDVIKKNLEIKDGPILGELLQYLSKELAYKRLNNFDEAIYKAKRWIEQN
APKCD

Sequences:

>Translated_405_residues
MKKSFHTDHTLIIDELETSIKVHNLDLILGFLPTGSYLVGGYIRDIILGRKTEKLDVDIVVPLNAIEIGKKIADNIRSKL
IILDKKREVVRIILNNIYIDIANQVSSSIEGDLCSRDLSINSIAFLFDKKSLFDPLNGLKDLEISLLRTHSENNLLNDPL
RILRCFRFVSELNFKIDSRLLDFIKKNKRNLHLVAKERITYEIQKIVNGANALDAVLLIKKLNIFDTENLFEDSFYLDLE
KINYGELDQEEQDKYLPSFFIAQILDVVTLEKLQFSKADIAKNKLLRKWHLLLKNKNIAQLDEFDRFKLHQELEMFLPSF
IFYLPENLQLDWLHRWRDNDDKLFHPSNIVNGDVIKKNLEIKDGPILGELLQYLSKELAYKRLNNFDEAIYKAKRWIEQN
APKCD
>Mature_405_residues
MKKSFHTDHTLIIDELETSIKVHNLDLILGFLPTGSYLVGGYIRDIILGRKTEKLDVDIVVPLNAIEIGKKIADNIRSKL
IILDKKREVVRIILNNIYIDIANQVSSSIEGDLCSRDLSINSIAFLFDKKSLFDPLNGLKDLEISLLRTHSENNLLNDPL
RILRCFRFVSELNFKIDSRLLDFIKKNKRNLHLVAKERITYEIQKIVNGANALDAVLLIKKLNIFDTENLFEDSFYLDLE
KINYGELDQEEQDKYLPSFFIAQILDVVTLEKLQFSKADIAKNKLLRKWHLLLKNKNIAQLDEFDRFKLHQELEMFLPSF
IFYLPENLQLDWLHRWRDNDDKLFHPSNIVNGDVIKKNLEIKDGPILGELLQYLSKELAYKRLNNFDEAIYKAKRWIEQN
APKCD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=204, Percent_Identity=27.9411764705882, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321016, Length=228, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47327; Mature: 47327

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSFHTDHTLIIDELETSIKVHNLDLILGFLPTGSYLVGGYIRDIILGRKTEKLDVDIV
CCCCCCCCCEEEEEHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VPLNAIEIGKKIADNIRSKLIILDKKREVVRIILNNIYIDIANQVSSSIEGDLCSRDLSI
ECCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHEEHHHHHHHCCCCHHCCCCCC
NSIAFLFDKKSLFDPLNGLKDLEISLLRTHSENNLLNDPLRILRCFRFVSELNFKIDSRL
CCEEEEECCHHHHHHHCCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
LDFIKKNKRNLHLVAKERITYEIQKIVNGANALDAVLLIKKLNIFDTENLFEDSFYLDLE
HHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCEEEEE
KINYGELDQEEQDKYLPSFFIAQILDVVTLEKLQFSKADIAKNKLLRKWHLLLKNKNIAQ
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
LDEFDRFKLHQELEMFLPSFIFYLPENLQLDWLHRWRDNDDKLFHPSNIVNGDVIKKNLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHCCCC
IKDGPILGELLQYLSKELAYKRLNNFDEAIYKAKRWIEQNAPKCD
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKSFHTDHTLIIDELETSIKVHNLDLILGFLPTGSYLVGGYIRDIILGRKTEKLDVDIV
CCCCCCCCCEEEEEHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VPLNAIEIGKKIADNIRSKLIILDKKREVVRIILNNIYIDIANQVSSSIEGDLCSRDLSI
ECCCHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHEEHHHHHHHCCCCHHCCCCCC
NSIAFLFDKKSLFDPLNGLKDLEISLLRTHSENNLLNDPLRILRCFRFVSELNFKIDSRL
CCEEEEECCHHHHHHHCCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
LDFIKKNKRNLHLVAKERITYEIQKIVNGANALDAVLLIKKLNIFDTENLFEDSFYLDLE
HHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCEEEEE
KINYGELDQEEQDKYLPSFFIAQILDVVTLEKLQFSKADIAKNKLLRKWHLLLKNKNIAQ
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
LDEFDRFKLHQELEMFLPSFIFYLPENLQLDWLHRWRDNDDKLFHPSNIVNGDVIKKNLE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEECCCCCCCCHHHHCCCC
IKDGPILGELLQYLSKELAYKRLNNFDEAIYKAKRWIEQNAPKCD
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]