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The map label for this gene is dus2 [H]

Identifier: 123967550

GI number: 123967550

Start: 16630

End: 17634

Strand: Reverse

Name: dus2 [H]

Synonym: A9601_00131

Alternate gene names: 123967550

Gene position: 17634-16630 (Counterclockwise)

Preceding gene: 123967556

Following gene: 123967543

Centisome position: 1.06

GC content: 30.55

Gene sequence:

>1005_bases
ATGAGTTACATTCAACATAATTCTATAAAAAATATTCATAAATTAAGTATTGCTCCAATGATGGACTGTACTGATAAACA
TTTCAGAATGATAATGAGAAAAATAAGTTCTGAAGCTCTGTTGTATACAGAAATGATTGTGGCCCAAAGTTTAGTTCATA
CGAATAAAAAAGAAAATTTTCTAGATTTTAATGGTGAAGAACATCCGATATCGATTCAGTTTGGTGGGGACGATCCTAAA
ATCCTTAAAGAGGCAGCTCGAATGGCGCAAGATTGGGGTTATGACGAAATAAACTTTAATGTTGGTTGTCCGAGTCCAAG
GGTCTGTTCTGGAAATTTTGGCGCTTCACTTATGAAAGACCCTGAAAAAGTAGCAAAATGTATAGAATCCTTAAAACTTA
ATTGCGATTTACCGGTTACGATCAAACACAGAATTGGTGTAGACAATGATGATAGTTTCGTTAAATTGAATAATTTTGTG
AGAATTGTTGCAAATGCTGGTGCAGACAGATTTACCGTTCATGCAAGAAAAGCCATATTAACAGGTCTAAATCCAAAACA
AAACAGGACCATACCTCCACTAAATTATGATGTAGTAAAAAAATTGAAAAATTTAAATCCAAGCTTATTAATAGAAATCA
ATGGGGGTTTAACAAATATAGATGAATCATTAAAAGCCTTAAATGATTTTGATGGAGTTATGATAGGACGGTCAATATAT
AAACATCCCTTAAGATGGTCTGAAATTGATCAAAAGATTTATGGAATTAATACAAAACCCAAATCTGCTTCGAATATTAT
ATTCTCCTTAATTCCATACATAGAAAAACATTTAAGTAATGGAGGAAAATCTTGGGATATTTGTAAACATCTTATAAATT
TAGTTGAAGGTATACCAAAAGCTAAAATTTGGAGAAACCAAATTTCAAATAAATCAATAAAAAAAGAATTAAATATTGAA
TATCTATTTAAATTAACAACAGCGCTTAAAGAAATGGGTTACTAA

Upstream 100 bases:

>100_bases
GCATAGGTTTAAAGAATATCTTTAAGATTGACATAGTAGGAATTAAAATAAAAATTCTTCTTGATAGAAATTGATTCATA
TATCTAAATCAAAAAAAATA

Downstream 100 bases:

>100_bases
TTTGTCTCGTTTGAAGCATTGTTCTCGTTGGACACTCCCCTCTTTCTCCTGCTCCTACCATTTTCATATTCAGAGTTTTC
AGAAGAATTTCCATAACTTC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSYIQHNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVHTNKKENFLDFNGEEHPISIQFGGDDPK
ILKEAARMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKLNCDLPVTIKHRIGVDNDDSFVKLNNFV
RIVANAGADRFTVHARKAILTGLNPKQNRTIPPLNYDVVKKLKNLNPSLLIEINGGLTNIDESLKALNDFDGVMIGRSIY
KHPLRWSEIDQKIYGINTKPKSASNIIFSLIPYIEKHLSNGGKSWDICKHLINLVEGIPKAKIWRNQISNKSIKKELNIE
YLFKLTTALKEMGY

Sequences:

>Translated_334_residues
MSYIQHNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVHTNKKENFLDFNGEEHPISIQFGGDDPK
ILKEAARMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKLNCDLPVTIKHRIGVDNDDSFVKLNNFV
RIVANAGADRFTVHARKAILTGLNPKQNRTIPPLNYDVVKKLKNLNPSLLIEINGGLTNIDESLKALNDFDGVMIGRSIY
KHPLRWSEIDQKIYGINTKPKSASNIIFSLIPYIEKHLSNGGKSWDICKHLINLVEGIPKAKIWRNQISNKSIKKELNIE
YLFKLTTALKEMGY
>Mature_333_residues
SYIQHNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVHTNKKENFLDFNGEEHPISIQFGGDDPKI
LKEAARMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKLNCDLPVTIKHRIGVDNDDSFVKLNNFVR
IVANAGADRFTVHARKAILTGLNPKQNRTIPPLNYDVVKKLKNLNPSLLIEINGGLTNIDESLKALNDFDGVMIGRSIYK
HPLRWSEIDQKIYGINTKPKSASNIIFSLIPYIEKHLSNGGKSWDICKHLINLVEGIPKAKIWRNQISNKSIKKELNIEY
LFKLTTALKEMGY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=232, Percent_Identity=28.448275862069, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=41.6129032258065, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=26.5560165975104, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=226, Percent_Identity=27.4336283185841, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=26.9461077844311, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=211, Percent_Identity=31.7535545023697, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=22.5108225108225, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37825; Mature: 37694

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIQHNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVHTNKKENF
CCCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
LDFNGEEHPISIQFGGDDPKILKEAARMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKLNCDLPVTIKHRIGVDNDDSFVKLNNFVRIVANAGADRFTVHARKAIL
HHHHHHHHHHHCCCCCCCEEEHEECCCCCCCCEEEHHHEEEEEECCCCCCEEHHHHHHHH
TGLNPKQNRTIPPLNYDVVKKLKNLNPSLLIEINGGLTNIDESLKALNDFDGVMIGRSIY
HCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHH
KHPLRWSEIDQKIYGINTKPKSASNIIFSLIPYIEKHLSNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
AKIWRNQISNKSIKKELNIEYLFKLTTALKEMGY
HHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYIQHNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVHTNKKENF
CCCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
LDFNGEEHPISIQFGGDDPKILKEAARMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKLNCDLPVTIKHRIGVDNDDSFVKLNNFVRIVANAGADRFTVHARKAIL
HHHHHHHHHHHCCCCCCCEEEHEECCCCCCCCEEEHHHEEEEEECCCCCCEEHHHHHHHH
TGLNPKQNRTIPPLNYDVVKKLKNLNPSLLIEINGGLTNIDESLKALNDFDGVMIGRSIY
HCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHH
KHPLRWSEIDQKIYGINTKPKSASNIIFSLIPYIEKHLSNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
AKIWRNQISNKSIKKELNIEYLFKLTTALKEMGY
HHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]