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The map label for this gene is speE

Identifier: 121999189

GI number: 121999189

Start: 2645778

End: 2646614

Strand: Reverse

Name: speE

Synonym: Hhal_2411

Alternate gene names: 121999189

Gene position: 2646614-2645778 (Counterclockwise)

Preceding gene: 121999192

Following gene: 121999183

Centisome position: 98.81

GC content: 69.53

Gene sequence:

>837_bases
ATGCGCGAGGGATGGTTTACCGAAGCGGTCGAGGACGAGGGCGTCGCCTTCTCTTTGGCCATCGACGAGCGGGTCCACGA
AGAGCAGAGCGAGTACCAGCACATCGAGGTCTACCGGACCCGGCGCTGGGGACGACTGCTGGTCCTCGACGGCTGCGTCA
TGCTCACCGAGCGGGACGAGTTCCTCTACCACGAGATGATGGCCCACCCGGCGCTCTTCGCCCACCGCGAGCCCCGGCGC
GTGGCCATCGTCGGCGGCGGCGACTGCGGCATCCTGCGCGAGGTGCTCCGCCATCCGGGCGTGGAGCACACCCTGCAGGT
GGAACTCGACGAGCGGGTGACCCGGGTCGCCGAGACCCACTTCCCGGACCTGTGCACCGCCAACGACGACGAGCGCGCCG
AGCTGCGTTTCACCGACGGCCTGCGCTGGATCCGCGAGGCGGAGCCGGAGTCGCTGGACGTGGTGATCGTCGACAGCACC
GACCCGGTCGGCCCGGCGGCGGGGCTGTTCACCAAGGAGTTCCTCACCGACGTGCGCAGCGCCCTGGGGCCCGGTGGCAT
TGTGGTCCAGCAGAGCGAGTCGCCACTGCTCCATCGCGATTCGGTCATCGCCCCGCTGCACCGCGCCGCCAGCGAGGCCG
GCTTCGACGGCGTGCACACCTTGACTTTCCCGGTGCCCAGCTACCCCTCGGGGTGGTGGTCGGCGACGCTGATGGTCAAC
GGCGGCGACCCGCGCAACTTCCGCGAGGCCGATTCGGAGGAGAAGCCCTTCGAGACCCGCTACTACAACGCCGACATCCA
CCGGGCGGCTCTGGCGGTGCCGGAACTGCTCAAGTAG

Upstream 100 bases:

>100_bases
GGAGAGACTGCGGCGCGACGCGCCACACTGCCCGAGGATGGTAACATTCGCGCATCCGCAGCGAGCGGAGCCGGACAACA
ACCCCATTGGCGGAGGCGCG

Downstream 100 bases:

>100_bases
TCGTGGAGGTGCCTGCGTCAGGCGGCCCCGCGGGGGCCTAGCGCAGGCGGTCCAGGCGCAGGCGTTCGCGCTCGTCGAAG
AGTCGGCGGGCGGCGAACCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MREGWFTEAVEDEGVAFSLAIDERVHEEQSEYQHIEVYRTRRWGRLLVLDGCVMLTERDEFLYHEMMAHPALFAHREPRR
VAIVGGGDCGILREVLRHPGVEHTLQVELDERVTRVAETHFPDLCTANDDERAELRFTDGLRWIREAEPESLDVVIVDST
DPVGPAAGLFTKEFLTDVRSALGPGGIVVQQSESPLLHRDSVIAPLHRAASEAGFDGVHTLTFPVPSYPSGWWSATLMVN
GGDPRNFREADSEEKPFETRYYNADIHRAALAVPELLK

Sequences:

>Translated_278_residues
MREGWFTEAVEDEGVAFSLAIDERVHEEQSEYQHIEVYRTRRWGRLLVLDGCVMLTERDEFLYHEMMAHPALFAHREPRR
VAIVGGGDCGILREVLRHPGVEHTLQVELDERVTRVAETHFPDLCTANDDERAELRFTDGLRWIREAEPESLDVVIVDST
DPVGPAAGLFTKEFLTDVRSALGPGGIVVQQSESPLLHRDSVIAPLHRAASEAGFDGVHTLTFPVPSYPSGWWSATLMVN
GGDPRNFREADSEEKPFETRYYNADIHRAALAVPELLK
>Mature_278_residues
MREGWFTEAVEDEGVAFSLAIDERVHEEQSEYQHIEVYRTRRWGRLLVLDGCVMLTERDEFLYHEMMAHPALFAHREPRR
VAIVGGGDCGILREVLRHPGVEHTLQVELDERVTRVAETHFPDLCTANDDERAELRFTDGLRWIREAEPESLDVVIVDST
DPVGPAAGLFTKEFLTDVRSALGPGGIVVQQSESPLLHRDSVIAPLHRAASEAGFDGVHTLTFPVPSYPSGWWSATLMVN
GGDPRNFREADSEEKPFETRYYNADIHRAALAVPELLK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=285, Percent_Identity=36.8421052631579, Blast_Score=201, Evalue=7e-52,
Organism=Escherichia coli, GI1786312, Length=265, Percent_Identity=35.0943396226415, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI25154106, Length=269, Percent_Identity=39.0334572490706, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI212645616, Length=319, Percent_Identity=32.9153605015674, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323175, Length=283, Percent_Identity=36.0424028268551, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325326, Length=279, Percent_Identity=35.4838709677419, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645443, Length=278, Percent_Identity=36.3309352517986, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=34.1232227488152, Blast_Score=127, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_HALHL (A1WZR2)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003976.1
- ProteinModelPortal:   A1WZR2
- SMR:   A1WZR2
- STRING:   A1WZR2
- GeneID:   4711427
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2411
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   VKHPLVE
- PhylomeDB:   A1WZR2
- ProtClustDB:   PRK00811
- BioCyc:   HHAL349124:HHAL_2411-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31369; Mature: 31369

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREGWFTEAVEDEGVAFSLAIDERVHEEQSEYQHIEVYRTRRWGRLLVLDGCVMLTERDE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEEEECEEEEECCHH
FLYHEMMAHPALFAHREPRRVAIVGGGDCGILREVLRHPGVEHTLQVELDERVTRVAETH
HHHHHHHHCCHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHC
FPDLCTANDDERAELRFTDGLRWIREAEPESLDVVIVDSTDPVGPAAGLFTKEFLTDVRS
CCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ALGPGGIVVQQSESPLLHRDSVIAPLHRAASEAGFDGVHTLTFPVPSYPSGWWSATLMVN
HCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEE
GGDPRNFREADSEEKPFETRYYNADIHRAALAVPELLK
CCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHC
>Mature Secondary Structure
MREGWFTEAVEDEGVAFSLAIDERVHEEQSEYQHIEVYRTRRWGRLLVLDGCVMLTERDE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEEEECEEEEECCHH
FLYHEMMAHPALFAHREPRRVAIVGGGDCGILREVLRHPGVEHTLQVELDERVTRVAETH
HHHHHHHHCCHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHC
FPDLCTANDDERAELRFTDGLRWIREAEPESLDVVIVDSTDPVGPAAGLFTKEFLTDVRS
CCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ALGPGGIVVQQSESPLLHRDSVIAPLHRAASEAGFDGVHTLTFPVPSYPSGWWSATLMVN
HCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEE
GGDPRNFREADSEEKPFETRYYNADIHRAALAVPELLK
CCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA