Click here to switch to the map view.

The map label for this gene is pfkA1 [H]

Identifier: 121999096

GI number: 121999096

Start: 2543148

End: 2544416

Strand: Reverse

Name: pfkA1 [H]

Synonym: Hhal_2318

Alternate gene names: 121999096

Gene position: 2544416-2543148 (Counterclockwise)

Preceding gene: 121999098

Following gene: 121999092

Centisome position: 95.0

GC content: 68.64

Gene sequence:

>1269_bases
ATGCCAAAGAACGCACTATATGCTCAGTCCGGTGGCGTCACGGCGGTCATCAACGCCAGCGCCTGGGGGGTGATCGAGGC
GGTGCGGGGTCACCCGGCCCATTTCGGCACCTGCTACGCGGCTCGTAACGGGATCCTCGGGGTGCTCGAGGAGGAGATCC
TCGATCTCGATGAGGAGGATCCGCTGACCCTGGCCGCCCTGCGCCATACCCCCGGGGGGGCGTTCGGGTCGTGCCGTTTC
GACCTCGGTGACTTGAAGCGGGATCGGGCGCAGTACGAGCGCCTGGTGGAGGTCTTCCACGCCCACGACATTGGCTACTT
CTTCTACAACGGCGGCGGTGGCTCCATGGACACCGCCCACAAGGTCGCGCGCATCGGCGACGAACTCGGCTACCCGATCA
CCGCCGTCGGCGTGCCCAAGACCATCGACAACGACCTGGGCATGACCGACACCAGTCCCGGGTTTGGATCGGCGGCCAAG
TTCATCGCCACGGTGGTCCGTGAGGCGAGCCTGGACGTCGAGTCGGCCCACACCACCTCGACGCGGGTCTTCATCCTCGA
GGTCATGGGGCGCCACGCCGGGTGGCTGGCGGCGGCAGCGGCGCTGGCCCAGGAGTACGACGGCCGGCCGCCGATGCTGA
TCCTGTTCCCCGAGACCCCCTTCGATCTCGGCGATTTTCTCACCCAGCTCCAGATGGCGGTCCGCACCCACGGCTACTGC
GTGGTGGTCGCCTCCGAGGGGCTCAAGAACCCCGATGGCTCCCTGTTCTCCGTGGCGCAGAGCCACGATATCTACGCCTA
CACCCAGCTAGGTGGCGTGGCGCCGCGGCTGGCCGAGATCATCCGCCAGGAGACCGGCCTTAAGCTGCACTGGTCGGTGG
TGGACTACATCCAGCGGGCGGCCCGGCATCTGGCCTCGCGGGTGGATGTGCAGCAGGCCTATGCGGTGGGGCGGTCCGCC
GTCGAGGGCGTGGTGGCCGGCGAGCACAGCTTCATGCCCGTCATCCGCCGGGATTCGGATCGGCCCTACCGGTGGTCCAT
CGACCGGGTGCCGCTGGAGGCGGTGGCGAGTATCGAGCGCGGGCTGCCGCCCGCGTTCATCTCGCCGGATGGCTACGGCA
TCACCGCCGCCTGTCGCGATTACCTGCGGCCGCTCATCGAGGGGGAGGACTATCCGCCGTTCGACCGCGGGCTGCCCTGT
TACCCGCGGATTCGCGGGGTGCTCCGCCCGCAGCGCTGCGCGCCGTTTCGCGTCGAGTCGCCGGGCTAA

Upstream 100 bases:

>100_bases
GAAATCCGCCGCCCATTGCCCGGCGGCGGCAAGCGTTCGGTGCATTCCTTCGGTACATTGGTGCGCAAGCCCATGAGCGT
CAACACACAAGTCTGATCGC

Downstream 100 bases:

>100_bases
CGCTCGGTCGGCGCCGCCCCGGGGCGTGACTCGAGGTGGGCGAGGATGGCGTCGGCGTAGAGGCCCGCGGTGTCCTGGGC
CGCCTGGACCGTGGCCGCAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: ATP-PFK; Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPKNALYAQSGGVTAVINASAWGVIEAVRGHPAHFGTCYAARNGILGVLEEEILDLDEEDPLTLAALRHTPGGAFGSCRF
DLGDLKRDRAQYERLVEVFHAHDIGYFFYNGGGGSMDTAHKVARIGDELGYPITAVGVPKTIDNDLGMTDTSPGFGSAAK
FIATVVREASLDVESAHTTSTRVFILEVMGRHAGWLAAAAALAQEYDGRPPMLILFPETPFDLGDFLTQLQMAVRTHGYC
VVVASEGLKNPDGSLFSVAQSHDIYAYTQLGGVAPRLAEIIRQETGLKLHWSVVDYIQRAARHLASRVDVQQAYAVGRSA
VEGVVAGEHSFMPVIRRDSDRPYRWSIDRVPLEAVASIERGLPPAFISPDGYGITAACRDYLRPLIEGEDYPPFDRGLPC
YPRIRGVLRPQRCAPFRVESPG

Sequences:

>Translated_422_residues
MPKNALYAQSGGVTAVINASAWGVIEAVRGHPAHFGTCYAARNGILGVLEEEILDLDEEDPLTLAALRHTPGGAFGSCRF
DLGDLKRDRAQYERLVEVFHAHDIGYFFYNGGGGSMDTAHKVARIGDELGYPITAVGVPKTIDNDLGMTDTSPGFGSAAK
FIATVVREASLDVESAHTTSTRVFILEVMGRHAGWLAAAAALAQEYDGRPPMLILFPETPFDLGDFLTQLQMAVRTHGYC
VVVASEGLKNPDGSLFSVAQSHDIYAYTQLGGVAPRLAEIIRQETGLKLHWSVVDYIQRAARHLASRVDVQQAYAVGRSA
VEGVVAGEHSFMPVIRRDSDRPYRWSIDRVPLEAVASIERGLPPAFISPDGYGITAACRDYLRPLIEGEDYPPFDRGLPC
YPRIRGVLRPQRCAPFRVESPG
>Mature_421_residues
PKNALYAQSGGVTAVINASAWGVIEAVRGHPAHFGTCYAARNGILGVLEEEILDLDEEDPLTLAALRHTPGGAFGSCRFD
LGDLKRDRAQYERLVEVFHAHDIGYFFYNGGGGSMDTAHKVARIGDELGYPITAVGVPKTIDNDLGMTDTSPGFGSAAKF
IATVVREASLDVESAHTTSTRVFILEVMGRHAGWLAAAAALAQEYDGRPPMLILFPETPFDLGDFLTQLQMAVRTHGYCV
VVASEGLKNPDGSLFSVAQSHDIYAYTQLGGVAPRLAEIIRQETGLKLHWSVVDYIQRAARHLASRVDVQQAYAVGRSAV
EGVVAGEHSFMPVIRRDSDRPYRWSIDRVPLEAVASIERGLPPAFISPDGYGITAACRDYLRPLIEGEDYPPFDRGLPCY
PRIRGVLRPQRCAPFRVESPG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=322, Percent_Identity=27.3291925465839, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558788, Length=285, Percent_Identity=28.4210526315789, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 45927; Mature: 45795

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNALYAQSGGVTAVINASAWGVIEAVRGHPAHFGTCYAARNGILGVLEEEILDLDEED
CCCCCEEECCCCEEEEEECCCCHHHHHHCCCCCHHEHHHHHCCCCHHHHHHHHHCCCCCC
PLTLAALRHTPGGAFGSCRFDLGDLKRDRAQYERLVEVFHAHDIGYFFYNGGGGSMDTAH
CEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
KVARIGDELGYPITAVGVPKTIDNDLGMTDTSPGFGSAAKFIATVVREASLDVESAHTTS
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TRVFILEVMGRHAGWLAAAAALAQEYDGRPPMLILFPETPFDLGDFLTQLQMAVRTHGYC
HHEEEEEHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEE
VVVASEGLKNPDGSLFSVAQSHDIYAYTQLGGVAPRLAEIIRQETGLKLHWSVVDYIQRA
EEEECCCCCCCCCCEEEECCCCCEEEEHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHHHH
ARHLASRVDVQQAYAVGRSAVEGVVAGEHSFMPVIRRDSDRPYRWSIDRVPLEAVASIER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEECCCCCHHHHHHHHH
GLPPAFISPDGYGITAACRDYLRPLIEGEDYPPFDRGLPCYPRIRGVLRPQRCAPFRVES
CCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEECCC
PG
CC
>Mature Secondary Structure 
PKNALYAQSGGVTAVINASAWGVIEAVRGHPAHFGTCYAARNGILGVLEEEILDLDEED
CCCCEEECCCCEEEEEECCCCHHHHHHCCCCCHHEHHHHHCCCCHHHHHHHHHCCCCCC
PLTLAALRHTPGGAFGSCRFDLGDLKRDRAQYERLVEVFHAHDIGYFFYNGGGGSMDTAH
CEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
KVARIGDELGYPITAVGVPKTIDNDLGMTDTSPGFGSAAKFIATVVREASLDVESAHTTS
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TRVFILEVMGRHAGWLAAAAALAQEYDGRPPMLILFPETPFDLGDFLTQLQMAVRTHGYC
HHEEEEEHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEE
VVVASEGLKNPDGSLFSVAQSHDIYAYTQLGGVAPRLAEIIRQETGLKLHWSVVDYIQRA
EEEECCCCCCCCCCEEEECCCCCEEEEHHHCCCCHHHHHHHHHHCCCEEEHHHHHHHHHH
ARHLASRVDVQQAYAVGRSAVEGVVAGEHSFMPVIRRDSDRPYRWSIDRVPLEAVASIER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEECCCCCHHHHHHHHH
GLPPAFISPDGYGITAACRDYLRPLIEGEDYPPFDRGLPCYPRIRGVLRPQRCAPFRVES
CCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEECCC
PG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055413; 12000953 [H]