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The map label for this gene is rpoH [H]

Identifier: 121999080

GI number: 121999080

Start: 2529404

End: 2530267

Strand: Reverse

Name: rpoH [H]

Synonym: Hhal_2302

Alternate gene names: 121999080

Gene position: 2530267-2529404 (Counterclockwise)

Preceding gene: 121999081

Following gene: 121999077

Centisome position: 94.47

GC content: 66.09

Gene sequence:

>864_bases
ATGAGCACGGCACTGGTCACGGCGGGTAACGCCAATCTGCCGCTGCCGACGAGCAGCGAGGACGCGTATATCCAGGCAGT
GAGCCAGATCCCGGTGCTCGATGCCGACGAGGAGTACGAGCTGGCCCGGCGGTGCCAGTACGAAGGCGATCTCGACGCCG
CCCGGCAGCTGGTGCTCTCGCATCTGCGTTTCGTCGTCCACGTGGCTCGGGGGTACCGCGGCTACGGCCTGGCCCTCGGT
GACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCCGTCAAGCGCTTCGATCCGGACGTGGGGGTTCGTCTGGTCTC
CTTCGCCGTGCACTGGATCCGCGCCGAGATCCACGAGTTCATCCTGCGCAACTGGCGCATCGTCAAGGTGGCCACCACCA
AGGCGCAGCGCAAACTGTTCTTCAACCTGCGTGGCGCCAAGAAGCGCCTGGGCTGGATGACCCGCGAAGAGGTCAACGCG
GTGGCCGAGGATCTCGGCGTCCAGCCTGAGACGGTCCTGGAGATGGAGTCCCGGCTCTCGGGGCAGGATCTGGGCTTCGA
TCCGCTGGTTTCCGAAGAAGATGAAGAGCAGGCGCGGGCCCCGGTGGCCTACCTGGAGGACCGGCGCTTCGATCCGGCCG
AGGTGGTGGAGAGTTCCGACTGGGGGACGCACCAGGACGATTCGCTGCACCAGGCGCTCGGTCAGCTGGACGAGCGCAGT
CAGAACATCCTGCGCGCCCGCTGGCTGGAGGAAGAGCGCCAGACCTTGCAGGAGCTGGCCGAGCGTTACGGTGTCTCTGC
CGAGCGCATCCGCCAGCTCGAGAAGAACGCCATCAGCAAACTGCGCGGGGCGGTCAGCGCCTGA

Upstream 100 bases:

>100_bases
AGCGCTGACATCGCCCCCAGCCGTGCCTTCGGAGAGGCAGAAACACCGGATTGGGTGGCTGGCTAGAGGAGTCTTGAACA
GGTTGGAGGAGAAGGCGCGT

Downstream 100 bases:

>100_bases
GCCGTCGCCTCATGGAGCGCTGCACCCCGCGCAGCGGATGTGCCCCCTACAGGACCCCCCGCTCGGCGAGTGACACCGGC
TCGCCGTCGCCCACCACGAA

Product: RNA polymerase sigma-32 subunit RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTALVTAGNANLPLPTSSEDAYIQAVSQIPVLDADEEYELARRCQYEGDLDAARQLVLSHLRFVVHVARGYRGYGLALG
DLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIRAEIHEFILRNWRIVKVATTKAQRKLFFNLRGAKKRLGWMTREEVNA
VAEDLGVQPETVLEMESRLSGQDLGFDPLVSEEDEEQARAPVAYLEDRRFDPAEVVESSDWGTHQDDSLHQALGQLDERS
QNILRARWLEEERQTLQELAERYGVSAERIRQLEKNAISKLRGAVSA

Sequences:

>Translated_287_residues
MSTALVTAGNANLPLPTSSEDAYIQAVSQIPVLDADEEYELARRCQYEGDLDAARQLVLSHLRFVVHVARGYRGYGLALG
DLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIRAEIHEFILRNWRIVKVATTKAQRKLFFNLRGAKKRLGWMTREEVNA
VAEDLGVQPETVLEMESRLSGQDLGFDPLVSEEDEEQARAPVAYLEDRRFDPAEVVESSDWGTHQDDSLHQALGQLDERS
QNILRARWLEEERQTLQELAERYGVSAERIRQLEKNAISKLRGAVSA
>Mature_286_residues
STALVTAGNANLPLPTSSEDAYIQAVSQIPVLDADEEYELARRCQYEGDLDAARQLVLSHLRFVVHVARGYRGYGLALGD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIRAEIHEFILRNWRIVKVATTKAQRKLFFNLRGAKKRLGWMTREEVNAV
AEDLGVQPETVLEMESRLSGQDLGFDPLVSEEDEEQARAPVAYLEDRRFDPAEVVESSDWGTHQDDSLHQALGQLDERSQ
NILRARWLEEERQTLQELAERYGVSAERIRQLEKNAISKLRGAVSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=61.1721611721612, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=31.1111111111111, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=28.6324786324786, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32467; Mature: 32336

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTALVTAGNANLPLPTSSEDAYIQAVSQIPVLDADEEYELARRCQYEGDLDAARQLVLS
CCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
HLRFVVHVARGYRGYGLALGDLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIRAEIHEF
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ILRNWRIVKVATTKAQRKLFFNLRGAKKRLGWMTREEVNAVAEDLGVQPETVLEMESRLS
HHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
GQDLGFDPLVSEEDEEQARAPVAYLEDRRFDPAEVVESSDWGTHQDDSLHQALGQLDERS
CCCCCCCCCCCCCCHHHHHCCHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QNILRARWLEEERQTLQELAERYGVSAERIRQLEKNAISKLRGAVSA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STALVTAGNANLPLPTSSEDAYIQAVSQIPVLDADEEYELARRCQYEGDLDAARQLVLS
CCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
HLRFVVHVARGYRGYGLALGDLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIRAEIHEF
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ILRNWRIVKVATTKAQRKLFFNLRGAKKRLGWMTREEVNAVAEDLGVQPETVLEMESRLS
HHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
GQDLGFDPLVSEEDEEQARAPVAYLEDRRFDPAEVVESSDWGTHQDDSLHQALGQLDERS
CCCCCCCCCCCCCCHHHHHCCHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QNILRARWLEEERQTLQELAERYGVSAERIRQLEKNAISKLRGAVSA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA