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The map label for this gene is dut

Identifier: 121999076

GI number: 121999076

Start: 2526400

End: 2526861

Strand: Reverse

Name: dut

Synonym: Hhal_2298

Alternate gene names: 121999076

Gene position: 2526861-2526400 (Counterclockwise)

Preceding gene: 121999077

Following gene: 121999075

Centisome position: 94.34

GC content: 68.61

Gene sequence:

>462_bases
ATGCACCGTAGGATCGAAGCACGCATCGTCGATGCACGTCTCGGCAGCGAGATCCCGCTGCCTGACTACGCCACCGACGG
CTCGGCCGCCATCGACCTGCGGGCCTGCCTGGACGAGCCGTTGGCCCTGGAGCCCGGGCAGCGACGGCTGATCGGTACCG
GGCTGGCGGTGAATATCCACGACCCCGGCCTGGTCGGTGTGGTGGCCTCGCGCTCGGGGCTCTCCCTCAAGCACGGCCTG
CGGGTAGCCCAGGGTATCGGCGTCATCGATTCCGACTACCATGGGGAGATCGGGGTGATCCTTGCCCACGACGGCACCGA
GCCCTATACGATCACCCCGGGGGAGCGCATCGCGCAGCTGCTCTTCCAGCCGGTGGTGCAGGTCACGCTGGACTACGTCT
CGGCGTTCTCGGCCACCACCGAGCGCGGCGAGGGCGGATTCGGCCACAGCGGCACGCTATGA

Upstream 100 bases:

>100_bases
AGCGTCGCCCTGGCCGAGCAGCCCAAGACGGCACTGGCCCGGGAGCTGCTGCAGATCGTGGCCGAGCGCGCCGGGTTCGC
GGAGGCAACGAGGAGCCACC

Downstream 100 bases:

>100_bases
CGGGGGAGTCCCCGCACCGAGAATGACAAGGAGGAGCCCGTGAGCGCAGCGCAAGTACCCGCATCGATCTTTCGTGCCTA
CGACGTCCGTGGCGTGGTGG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MHRRIEARIVDARLGSEIPLPDYATDGSAAIDLRACLDEPLALEPGQRRLIGTGLAVNIHDPGLVGVVASRSGLSLKHGL
RVAQGIGVIDSDYHGEIGVILAHDGTEPYTITPGERIAQLLFQPVVQVTLDYVSAFSATTERGEGGFGHSGTL

Sequences:

>Translated_153_residues
MHRRIEARIVDARLGSEIPLPDYATDGSAAIDLRACLDEPLALEPGQRRLIGTGLAVNIHDPGLVGVVASRSGLSLKHGL
RVAQGIGVIDSDYHGEIGVILAHDGTEPYTITPGERIAQLLFQPVVQVTLDYVSAFSATTERGEGGFGHSGTL
>Mature_153_residues
MHRRIEARIVDARLGSEIPLPDYATDGSAAIDLRACLDEPLALEPGQRRLIGTGLAVNIHDPGLVGVVASRSGLSLKHGL
RVAQGIGVIDSDYHGEIGVILAHDGTEPYTITPGERIAQLLFQPVVQVTLDYVSAFSATTERGEGGFGHSGTL

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=36.4285714285714, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI70906444, Length=141, Percent_Identity=36.1702127659575, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI70906441, Length=141, Percent_Identity=36.1702127659575, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=51.006711409396, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71988561, Length=133, Percent_Identity=38.3458646616541, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319729, Length=90, Percent_Identity=37.7777777777778, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921126, Length=140, Percent_Identity=33.5714285714286, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583610, Length=140, Percent_Identity=33.5714285714286, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_HALHL (A1WZE9)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003863.1
- ProteinModelPortal:   A1WZE9
- SMR:   A1WZE9
- STRING:   A1WZE9
- GeneID:   4709444
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2298
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   A1WZE9
- BioCyc:   HHAL349124:HHAL_2298-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16162; Mature: 16162

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 84-84

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRIEARIVDARLGSEIPLPDYATDGSAAIDLRACLDEPLALEPGQRRLIGTGLAVNIH
CCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCEEEECCEEEEEC
DPGLVGVVASRSGLSLKHGLRVAQGIGVIDSDYHGEIGVILAHDGTEPYTITPGERIAQL
CCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCCCCEEEEEEECCCCCEEECCHHHHHHH
LFQPVVQVTLDYVSAFSATTERGEGGFGHSGTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRRIEARIVDARLGSEIPLPDYATDGSAAIDLRACLDEPLALEPGQRRLIGTGLAVNIH
CCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCEEEECCEEEEEC
DPGLVGVVASRSGLSLKHGLRVAQGIGVIDSDYHGEIGVILAHDGTEPYTITPGERIAQL
CCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCCCCEEEEEEECCCCCEEECCHHHHHHH
LFQPVVQVTLDYVSAFSATTERGEGGFGHSGTL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA