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The map label for this gene is aspS

Identifier: 121999005

GI number: 121999005

Start: 2439816

End: 2441669

Strand: Reverse

Name: aspS

Synonym: Hhal_2226

Alternate gene names: 121999005

Gene position: 2441669-2439816 (Counterclockwise)

Preceding gene: 121999006

Following gene: 121999003

Centisome position: 91.16

GC content: 68.34

Gene sequence:

>1854_bases
TTGCGCCCCCCCGGGGGCGTGCTTACCATTGCCACCCTTTGGCCCCACCTACCCAATCCGGAATCAACCATGCGTAGTCA
CTACTGCGGCGAGATCTGCGAATCCCACCTGGATACCGAAGTCACTCTGTGCGGCTGGGTGCACCGGCGGCGCGATCACG
GCGGCATCATCTTCATCGACCTGCGCGATCGGGCCGGTCTGGTGCAGGTGGTGGTCGATCCGGACACCGAAGAGGCGTTC
GCCGCCGCCGACCGCGCGCGGAACGAGTACGTCCTGCGCATCACCGGTCGGGTGCGGCACCGGCCGGAGGGTACCGAAAA
CCCGGATCTGCACTCCGGGCGGGTCGAGGTCCTGGGCCGGGCGGTCGAGGTGCTCAACACCGCCAAGACGCCGCCGTTCC
AGCTCGATGAGCACGAGCAGGTGGGCGAGGACGTGCGCCTGCGTCACCGCTACGTGGATCTGCGTCGCCCCGAGATGCAG
CAGCGCCTGCAGCTGCGCGCCCGGGTGAGTTCGGCCATCCGCCGCCACCTGGAGGACGAGGGGTTCCTCGACATCGAGAC
GCCGATGCTGACTCGGGCCACGCCCGAGGGCGCCCGCGACTATCTGGTGCCCAGCCGCACCCACCCCGGCCGTTTCTTCG
CGCTGCCGCAGTCGCCGCAGCTGTTCAAGCAGTTGCTGATGATGGCCGGGTTCGACCGCTACTACCAGATCGTGCGCTGC
TTCCGTGACGAGGACCTGCGCGCCGATCGCCAGCCGGAGTTCACCCAGCTCGACATGGAGGCCGCCTTCGTCGACGAAGA
GGCCGTCATGGGGGTCACCGAGCGGATGCTGCGCCAGCTCTTCCGCCAGGTTCTGGGCGTCGAGCTGTCGGATCCGTTCC
CGCGCATGCGTTACGCCGAAGCCATGGATCGGTACGGCTCGGACAAGCCGGACCTGCGCATCCCGCTGGAGCTGGTCGAG
GTCGGCGACCTGGTCCGCGAGGTGGATTTCAAGGTCTTCTCGGCGCCGGCCCAAGATCCGCGGGGCCGTGTGGCGGCCCT
GCGTGTCCCCGGTGGGGGCGGGCTGACGCGCAAGCAGATCGACGACTACACCGATTTCGTCGGCCGTTACGGAGCCAAGG
GCCTGGCCTACATCAAGGTCAACGACCCGGCTCAGGGGGTCGAGGGCCTGCAGTCGCCCATCGTCAAGTTCCTCACCGAG
GAGGCCGTGCGGGGAATCCTCGAGCGCACGGGTGCCCAGGCGGGCGACGTGGTCTTCTTTGGCGCCGATCGGGCCAGCGT
GGTCAACGACGCCCTGGGGGCATTGCGCGTGCGCATCGGCCACGACATGGGGCTGGTCGAGGACGCCTGGCGGCCGCTGT
GGGTGGTCGATTTCCCGATGTTCGAGTACGACGAGAAGGACGGGCGGCTCTACTCCCTGCACCATCCGTTTACTGCCCCG
TGCGTACAGGACCCCGCCGAGCTTCAGCAGCAGGACGCCGAGGCCCTGGTCTCGCGAGCGTACGACTGTGTGCTCAACGG
CGTTGAACTCGGCGGCGGGTCGATCCGTATCCACGATCCCGACATGCAGCAGGCCGTCTTCGGGGTGCTGGGTATCGGCG
AGGAGGAGGCCCGCTCCAAGTTCGGGTTCCTGCTCGACGCCCTGCAGTACGGCTGCCCGCCCCACGGCGGTATCGCCTTC
GGCATGGACCGGCTGGTGATGCTCATGGCGGGGGCCGACTCGATTCGCGAGGTCATGGCCTTCCCCAAGACCCAGACCGC
CACCTGCATGCTCACCGAGGCGCCGGCCGAGGTGGGCGAGGCGCAGTTGCGTGAGCTGGGCATCCGCCTGCGGCGGGTCA
GCGGTTCGGAGTAA

Upstream 100 bases:

>100_bases
GTTCGGCGTGTTGTCGGCGATCTCTCCGAGCGCGAGAAGGTGCTCATCGGGCTCGACCTCAAGGAGGGGTGAGTAGCCGC
TGCCGTGATCGGGTCCTCGC

Downstream 100 bases:

>100_bases
CCGCCGGCGCGGTCCCGGTCAGCCGAGCCAACTGCCGTAGCTGGCGTAGCGCAGCGCCAGACGTTTGGCCGGTCCGCCGT
GATCGGCCAGGCGCAGTCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MRPPGGVLTIATLWPHLPNPESTMRSHYCGEICESHLDTEVTLCGWVHRRRDHGGIIFIDLRDRAGLVQVVVDPDTEEAF
AAADRARNEYVLRITGRVRHRPEGTENPDLHSGRVEVLGRAVEVLNTAKTPPFQLDEHEQVGEDVRLRHRYVDLRRPEMQ
QRLQLRARVSSAIRRHLEDEGFLDIETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMMAGFDRYYQIVRC
FRDEDLRADRQPEFTQLDMEAAFVDEEAVMGVTERMLRQLFRQVLGVELSDPFPRMRYAEAMDRYGSDKPDLRIPLELVE
VGDLVREVDFKVFSAPAQDPRGRVAALRVPGGGGLTRKQIDDYTDFVGRYGAKGLAYIKVNDPAQGVEGLQSPIVKFLTE
EAVRGILERTGAQAGDVVFFGADRASVVNDALGALRVRIGHDMGLVEDAWRPLWVVDFPMFEYDEKDGRLYSLHHPFTAP
CVQDPAELQQQDAEALVSRAYDCVLNGVELGGGSIRIHDPDMQQAVFGVLGIGEEEARSKFGFLLDALQYGCPPHGGIAF
GMDRLVMLMAGADSIREVMAFPKTQTATCMLTEAPAEVGEAQLRELGIRLRRVSGSE

Sequences:

>Translated_617_residues
MRPPGGVLTIATLWPHLPNPESTMRSHYCGEICESHLDTEVTLCGWVHRRRDHGGIIFIDLRDRAGLVQVVVDPDTEEAF
AAADRARNEYVLRITGRVRHRPEGTENPDLHSGRVEVLGRAVEVLNTAKTPPFQLDEHEQVGEDVRLRHRYVDLRRPEMQ
QRLQLRARVSSAIRRHLEDEGFLDIETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMMAGFDRYYQIVRC
FRDEDLRADRQPEFTQLDMEAAFVDEEAVMGVTERMLRQLFRQVLGVELSDPFPRMRYAEAMDRYGSDKPDLRIPLELVE
VGDLVREVDFKVFSAPAQDPRGRVAALRVPGGGGLTRKQIDDYTDFVGRYGAKGLAYIKVNDPAQGVEGLQSPIVKFLTE
EAVRGILERTGAQAGDVVFFGADRASVVNDALGALRVRIGHDMGLVEDAWRPLWVVDFPMFEYDEKDGRLYSLHHPFTAP
CVQDPAELQQQDAEALVSRAYDCVLNGVELGGGSIRIHDPDMQQAVFGVLGIGEEEARSKFGFLLDALQYGCPPHGGIAF
GMDRLVMLMAGADSIREVMAFPKTQTATCMLTEAPAEVGEAQLRELGIRLRRVSGSE
>Mature_617_residues
MRPPGGVLTIATLWPHLPNPESTMRSHYCGEICESHLDTEVTLCGWVHRRRDHGGIIFIDLRDRAGLVQVVVDPDTEEAF
AAADRARNEYVLRITGRVRHRPEGTENPDLHSGRVEVLGRAVEVLNTAKTPPFQLDEHEQVGEDVRLRHRYVDLRRPEMQ
QRLQLRARVSSAIRRHLEDEGFLDIETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMMAGFDRYYQIVRC
FRDEDLRADRQPEFTQLDMEAAFVDEEAVMGVTERMLRQLFRQVLGVELSDPFPRMRYAEAMDRYGSDKPDLRIPLELVE
VGDLVREVDFKVFSAPAQDPRGRVAALRVPGGGGLTRKQIDDYTDFVGRYGAKGLAYIKVNDPAQGVEGLQSPIVKFLTE
EAVRGILERTGAQAGDVVFFGADRASVVNDALGALRVRIGHDMGLVEDAWRPLWVVDFPMFEYDEKDGRLYSLHHPFTAP
CVQDPAELQQQDAEALVSRAYDCVLNGVELGGGSIRIHDPDMQQAVFGVLGIGEEEARSKFGFLLDALQYGCPPHGGIAF
GMDRLVMLMAGADSIREVMAFPKTQTATCMLTEAPAEVGEAQLRELGIRLRRVSGSE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=38.8797364085667, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=300, Percent_Identity=26, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI194272210, Length=300, Percent_Identity=26, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45439306, Length=335, Percent_Identity=27.1641791044776, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI4758762, Length=307, Percent_Identity=25.7328990228013, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=59.8639455782313, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=27.6450511945392, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789256, Length=298, Percent_Identity=26.8456375838926, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787161, Length=273, Percent_Identity=27.1062271062271, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082379, Length=168, Percent_Identity=30.952380952381, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32566633, Length=610, Percent_Identity=33.9344262295082, Blast_Score=329, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=299, Percent_Identity=22.4080267558528, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=299, Percent_Identity=22.4080267558528, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=299, Percent_Identity=22.4080267558528, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=290, Percent_Identity=26.8965517241379, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=27.3381294964029, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=626, Percent_Identity=33.5463258785943, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=28.8, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=351, Percent_Identity=23.6467236467236, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=25, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=38.1270903010033, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=28.0155642023346, Blast_Score=97, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24640851, Length=261, Percent_Identity=27.9693486590038, Blast_Score=97, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17136276, Length=237, Percent_Identity=29.535864978903, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921528, Length=284, Percent_Identity=28.169014084507, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 69228; Mature: 69228

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPGGVLTIATLWPHLPNPESTMRSHYCGEICESHLDTEVTLCGWVHRRRDHGGIIFID
CCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEE
LRDRAGLVQVVVDPDTEEAFAAADRARNEYVLRITGRVRHRPEGTENPDLHSGRVEVLGR
ECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AVEVLNTAKTPPFQLDEHEQVGEDVRLRHRYVDLRRPEMQQRLQLRARVSSAIRRHLEDE
HHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
GFLDIETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMMAGFDRYYQIVRC
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHH
FRDEDLRADRQPEFTQLDMEAAFVDEEAVMGVTERMLRQLFRQVLGVELSDPFPRMRYAE
HCCCCCCCCCCCCCCEECHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AMDRYGSDKPDLRIPLELVEVGDLVREVDFKVFSAPAQDPRGRVAALRVPGGGGLTRKQI
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCHHHH
DDYTDFVGRYGAKGLAYIKVNDPAQGVEGLQSPIVKFLTEEAVRGILERTGAQAGDVVFF
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GADRASVVNDALGALRVRIGHDMGLVEDAWRPLWVVDFPMFEYDEKDGRLYSLHHPFTAP
CCCHHHHHHHHHHHHHHEECCCCCCHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCC
CVQDPAELQQQDAEALVSRAYDCVLNGVELGGGSIRIHDPDMQQAVFGVLGIGEEEARSK
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCHHHHHHH
FGFLLDALQYGCPPHGGIAFGMDRLVMLMAGADSIREVMAFPKTQTATCMLTEAPAEVGE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCHHHHH
AQLRELGIRLRRVSGSE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPPGGVLTIATLWPHLPNPESTMRSHYCGEICESHLDTEVTLCGWVHRRRDHGGIIFID
CCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEE
LRDRAGLVQVVVDPDTEEAFAAADRARNEYVLRITGRVRHRPEGTENPDLHSGRVEVLGR
ECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AVEVLNTAKTPPFQLDEHEQVGEDVRLRHRYVDLRRPEMQQRLQLRARVSSAIRRHLEDE
HHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
GFLDIETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMMAGFDRYYQIVRC
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHH
FRDEDLRADRQPEFTQLDMEAAFVDEEAVMGVTERMLRQLFRQVLGVELSDPFPRMRYAE
HCCCCCCCCCCCCCCEECHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AMDRYGSDKPDLRIPLELVEVGDLVREVDFKVFSAPAQDPRGRVAALRVPGGGGLTRKQI
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCHHHH
DDYTDFVGRYGAKGLAYIKVNDPAQGVEGLQSPIVKFLTEEAVRGILERTGAQAGDVVFF
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GADRASVVNDALGALRVRIGHDMGLVEDAWRPLWVVDFPMFEYDEKDGRLYSLHHPFTAP
CCCHHHHHHHHHHHHHHEECCCCCCHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCC
CVQDPAELQQQDAEALVSRAYDCVLNGVELGGGSIRIHDPDMQQAVFGVLGIGEEEARSK
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCHHHHHHHHHCCCHHHHHHH
FGFLLDALQYGCPPHGGIAFGMDRLVMLMAGADSIREVMAFPKTQTATCMLTEAPAEVGE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCHHHHH
AQLRELGIRLRRVSGSE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA