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The map label for this gene is rfbB [C]

Identifier: 121998958

GI number: 121998958

Start: 2390909

End: 2391919

Strand: Reverse

Name: rfbB [C]

Synonym: Hhal_2179

Alternate gene names: 121998958

Gene position: 2391919-2390909 (Counterclockwise)

Preceding gene: 121998959

Following gene: 121998957

Centisome position: 89.3

GC content: 65.48

Gene sequence:

>1011_bases
GTGCGAATCCTCGTCACCGGCGTGGCCGGTTTCATCGGCATGCACTGCGCCCGGCAGCTGATCGAGGCGGGCCATCAAGT
CGTCGGAATCGATAATCTCAACGACTACTACGACGTAACCCTCAAGGAGGCGCGCCTGGACGAGCTGCGCCGCTGCGAGG
GGGATTTCTACTTCGCCCGCGTAGACCTCGCCGATTCCGCCGGCGTCGACGCCCTCTTCCGCGAGGGGCGCTTCGACCGC
GTCATCCATCTGGCCGCCCAGGCCGGCGTGCGCTATTCCCTGGAGAATCCGCGGGCGTACATCGACAGCAATCTGGTGGG
CTTCGGCAACATCCTGGAGGGGTGCCGTCACCACGACACCGGGCACCTGGTCTACGCCTCTTCGAGTTCAGTCTACGGCG
CCAACACGCGTATGCCGTTCTCGGTCCACGACAACGTCGATCACCCGGTGTCGCTCTACGCGGCCACCAAGAAGTCCAAC
GAGCTGATGGCGCACACCTACGCCCACCTCTACGGTCTGCCCGTCACCGGTCTGCGCTTCTTCACCGTCTACGGCCCCTG
GGGGCGTCCGGACATGGCGCCGTTCAAGTTTACGCGGTCGATCCTCGCCGGAGAGCCTATCGAGGTCTACAACTACGGCC
GCATGCGCCGCGACTTCACCTACATCGACGATATCGTCGACGGGGTGCTGCGCGTGATGGACACCCTGCCGGAGCCGGAT
CCGGAGTTCTCCACCGATGCCCCGGATCCGGCGCGCAGCAACGCGCCCTACCGCGTCTACAATATCGGCAACCATCGGCC
TGTGGCGCTCGAGGACTTCATCGCCGCCCTGGAGGACGCCTGCGGGCGGAAGGCGCAGCGGCACGAGCTGCCCATGCAGC
CAGGAGACGTCGCCGAGACCTACGCCGACATCGACGATCTGACCGCCGCGACGGGGTGGCACCCGCAGACGGCCATCGAG
CAGGGTTTACCGCAGTTCGTGGCTTGGTATCGAGCGTTTTACGGGGTCTGA

Upstream 100 bases:

>100_bases
GGCTGGCGGCTAGGCGGGTCCGCCCTGTACGGCGAATCGGGCCTCGGGTACCCTAAAGGCCGTTTTTCCCCGGTGTAACT
GCGCGAGAGGAAGGAAACCC

Downstream 100 bases:

>100_bases
GAGGCGGCCCCTCAAGGAGTGGAGTGAATCATGAAGGTCACGATCTTCGGCAGCGGCTACGTGGGTCTGGTTACCGGGAC
CTGCTTCGCCGATGCCGGCA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRILVTGVAGFIGMHCARQLIEAGHQVVGIDNLNDYYDVTLKEARLDELRRCEGDFYFARVDLADSAGVDALFREGRFDR
VIHLAAQAGVRYSLENPRAYIDSNLVGFGNILEGCRHHDTGHLVYASSSSVYGANTRMPFSVHDNVDHPVSLYAATKKSN
ELMAHTYAHLYGLPVTGLRFFTVYGPWGRPDMAPFKFTRSILAGEPIEVYNYGRMRRDFTYIDDIVDGVLRVMDTLPEPD
PEFSTDAPDPARSNAPYRVYNIGNHRPVALEDFIAALEDACGRKAQRHELPMQPGDVAETYADIDDLTAATGWHPQTAIE
QGLPQFVAWYRAFYGV

Sequences:

>Translated_336_residues
MRILVTGVAGFIGMHCARQLIEAGHQVVGIDNLNDYYDVTLKEARLDELRRCEGDFYFARVDLADSAGVDALFREGRFDR
VIHLAAQAGVRYSLENPRAYIDSNLVGFGNILEGCRHHDTGHLVYASSSSVYGANTRMPFSVHDNVDHPVSLYAATKKSN
ELMAHTYAHLYGLPVTGLRFFTVYGPWGRPDMAPFKFTRSILAGEPIEVYNYGRMRRDFTYIDDIVDGVLRVMDTLPEPD
PEFSTDAPDPARSNAPYRVYNIGNHRPVALEDFIAALEDACGRKAQRHELPMQPGDVAETYADIDDLTAATGWHPQTAIE
QGLPQFVAWYRAFYGV
>Mature_336_residues
MRILVTGVAGFIGMHCARQLIEAGHQVVGIDNLNDYYDVTLKEARLDELRRCEGDFYFARVDLADSAGVDALFREGRFDR
VIHLAAQAGVRYSLENPRAYIDSNLVGFGNILEGCRHHDTGHLVYASSSSVYGANTRMPFSVHDNVDHPVSLYAATKKSN
ELMAHTYAHLYGLPVTGLRFFTVYGPWGRPDMAPFKFTRSILAGEPIEVYNYGRMRRDFTYIDDIVDGVLRVMDTLPEPD
PEFSTDAPDPARSNAPYRVYNIGNHRPVALEDFIAALEDACGRKAQRHELPMQPGDVAETYADIDDLTAATGWHPQTAIE
QGLPQFVAWYRAFYGV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=26.9230769230769, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=24.2603550295858, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI56237023, Length=354, Percent_Identity=25.9887005649718, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56118217, Length=354, Percent_Identity=25.9887005649718, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI189083684, Length=354, Percent_Identity=25.9887005649718, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=26.0504201680672, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=27.9661016949153, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786974, Length=358, Percent_Identity=24.5810055865922, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788366, Length=201, Percent_Identity=26.3681592039801, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=26.4204545454545, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982035, Length=350, Percent_Identity=26.5714285714286, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=26.9005847953216, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=23.2352941176471, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=22.2222222222222, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=25.7790368271955, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=27.2206303724928, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37631; Mature: 37631

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGVAGFIGMHCARQLIEAGHQVVGIDNLNDYYDVTLKEARLDELRRCEGDFYFAR
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEEHHHHHHHHHHCCCCEEEEE
VDLADSAGVDALFREGRFDRVIHLAAQAGVRYSLENPRAYIDSNLVGFGNILEGCRHHDT
EECCCCCCHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHCCCCCCHHHHHHHHHCCCC
GHLVYASSSSVYGANTRMPFSVHDNVDHPVSLYAATKKSNELMAHTYAHLYGLPVTGLRF
CEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
FTVYGPWGRPDMAPFKFTRSILAGEPIEVYNYGRMRRDFTYIDDIVDGVLRVMDTLPEPD
EEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PEFSTDAPDPARSNAPYRVYNIGNHRPVALEDFIAALEDACGRKAQRHELPMQPGDVAET
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
YADIDDLTAATGWHPQTAIEQGLPQFVAWYRAFYGV
HHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRILVTGVAGFIGMHCARQLIEAGHQVVGIDNLNDYYDVTLKEARLDELRRCEGDFYFAR
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEEHHHHHHHHHHCCCCEEEEE
VDLADSAGVDALFREGRFDRVIHLAAQAGVRYSLENPRAYIDSNLVGFGNILEGCRHHDT
EECCCCCCHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHCCCCCCHHHHHHHHHCCCC
GHLVYASSSSVYGANTRMPFSVHDNVDHPVSLYAATKKSNELMAHTYAHLYGLPVTGLRF
CEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
FTVYGPWGRPDMAPFKFTRSILAGEPIEVYNYGRMRRDFTYIDDIVDGVLRVMDTLPEPD
EEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PEFSTDAPDPARSNAPYRVYNIGNHRPVALEDFIAALEDACGRKAQRHELPMQPGDVAET
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
YADIDDLTAATGWHPQTAIEQGLPQFVAWYRAFYGV
HHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]