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The map label for this gene is mdeA [H]

Identifier: 121998932

GI number: 121998932

Start: 2362230

End: 2363498

Strand: Reverse

Name: mdeA [H]

Synonym: Hhal_2153

Alternate gene names: 121998932

Gene position: 2363498-2362230 (Counterclockwise)

Preceding gene: 121998934

Following gene: 121998930

Centisome position: 88.24

GC content: 66.51

Gene sequence:

>1269_bases
TTGAAACTCGAGACCCAGGCCGTCCACGCCGGCTACAGCCCCGATCCGACCACCCGAGCGGTGGCAGTGCCCATCTATCA
GACCACCTCCTACGCCTTCGATGACACGCAGCACGGGGCGGATCTCTTCGACCTCAAGGTCGAGGGCAATATCTATACAC
GGATCATGAACCCCACCAACGCCGTGCTCGAGCAGCGCGTGGCGGCTATGGAGGGGGGCATCGGTGGGCTGGCGGTGGCC
TCCGGCATGGCGGCGATCACCTACGCCATCCAGGCGATCACCCGCGCCGGCGACAACATCGTCTCCACCTCGCGGCTCTA
CGGCGGCACCTACAACCTCTTCGCCCACTCGCTGCCCATCCACGGCATCGAGGTTCGCTTCGCCGCCCAAGACGACTACG
AGCGCCTCGAGGCGCAGATCGACGAGCGCACCAAGGCGCTCTTCTGCGAGAGTGTCGGCAACCCCTCTGGTGAGGTGGTG
GACGTCGAGCGGCTGGCCGAGATCGCCCACCGACACGGCATCCCGCTGATGGTCGACAACACCGTGCCCACGCCCTATCT
GTGGCGCCCCATCGACAACGGCGCGGACATCGTCATCCACTCGCTGACCAAGTACATGGGTGGGCACGGCACCACGGTGG
GCGGGGTGATCGTCGACTCCGGGAAGTTCCCCTGGGCCGACCACGGAGACCGCTTCCCGATGATGGTCGAGCCGGACCCC
TCCTACCATGGGGTGGTTTACACCGAGGATATGGGCGAGGCGGCCTACATCACCCGCTGCCGGGTGGTGCCGTTGCGCAA
CATGGGGGCCGCGCTGTCGCCGTTCAACGCCTTCCAGCTGCTGCAGGGGATCGAGACCCTGCCGGTGCGTATGGACCGCC
ACTGCGACAACGCCCAGCGGGTTGCCGAGTACCTGCAGGCCCATCCGCGGGTAAGCTGGGTGAAGTTCGCCGGCCTCGAG
GACAGCCCCTACAAGCCCCTGGCTGATCGCTACATGGGCGGCCGGGCGGCCAGCATCCTGAGTTTCGGGGTGCAGGGCGG
CTTCGAGGCGGGGGCACGCTTCATCGATGCCCTGCAGCTGATCACCCGGCTGGTGAACATCGGTGATGCCAAGTCCCTGG
CTTGCCACCCGGCCAGCACCACGCACCGCCAGCTCAACGATGAGGAGCTGGAGAGCGCAGGGGTATCGCGGGATCTGGTG
CGCCTGTCCATCGGCCTGGAGCACGTCGACGACATCCTGGCGGATATCGACCAGGCCTTGCAGGCGTAA

Upstream 100 bases:

>100_bases
CCGCAATGGCGATGCCACTTTTGGATCAGAGTGCGCGCGCCTTCGGGCCGGAGCCGGTCCGGGCGCCCGATCCGGAATCT
CGCAAAAGGAGTCATCCGCG

Downstream 100 bases:

>100_bases
CGTCTGTAGGGCTTCAGGGGCTGGTGTCTAGCCGAGCCCCTGTGCCTGAGCCGCTGGCGGCGGCGCGCAGCCGCCGCCAG
CGAGGCAGCGTGTTGCGCCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTNAVLEQRVAAMEGGIGGLAVA
SGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPIHGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVV
DVERLAEIAHRHGIPLMVDNTVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQRVAEYLQAHPRVSWVKFAGLE
DSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQLITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLV
RLSIGLEHVDDILADIDQALQA

Sequences:

>Translated_422_residues
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTNAVLEQRVAAMEGGIGGLAVA
SGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPIHGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVV
DVERLAEIAHRHGIPLMVDNTVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQRVAEYLQAHPRVSWVKFAGLE
DSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQLITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLV
RLSIGLEHVDDILADIDQALQA
>Mature_422_residues
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTNAVLEQRVAAMEGGIGGLAVA
SGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPIHGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVV
DVERLAEIAHRHGIPLMVDNTVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQRVAEYLQAHPRVSWVKFAGLE
DSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQLITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLV
RLSIGLEHVDDILADIDQALQA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=34.916864608076, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=31.353919239905, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.4761904761905, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=31.7535545023697, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=27.5943396226415, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=431, Percent_Identity=29.4663573085847, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=32.4644549763033, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=31.2796208530806, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=48.6111111111111, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=31.6901408450704, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=25.6124721603563, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=215, Percent_Identity=30.6976744186047, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=437, Percent_Identity=22.883295194508, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=32.2274881516588, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45994; Mature: 45994

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTN
CCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECHHH
AVLEQRVAAMEGGIGGLAVASGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHCCCC
HGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVVDVERLAEIAHRHGIPLMVDN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEECC
TVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCCCCCCCCCCEEECCCC
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQR
CCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHCHHHCCHHHHCCCCHHHH
VAEYLQAHPRVSWVKFAGLEDSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQL
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLVRLSIGLEHVDDILADIDQAL
HHHHHCCCCCCCEEECCCCCHHHCCCHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHH
QA
CC
>Mature Secondary Structure
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTN
CCCCCCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECHHH
AVLEQRVAAMEGGIGGLAVASGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHCCCC
HGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVVDVERLAEIAHRHGIPLMVDN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCEEECC
TVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCCCCCCCCCCEEECCCC
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQR
CCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHCHHHCCHHHHCCCCHHHH
VAEYLQAHPRVSWVKFAGLEDSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQL
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLVRLSIGLEHVDDILADIDQAL
HHHHHCCCCCCCEEECCCCCHHHCCCHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHH
QA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]