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The map label for this gene is yafV [C]

Identifier: 121998914

GI number: 121998914

Start: 2334624

End: 2335484

Strand: Reverse

Name: yafV [C]

Synonym: Hhal_2135

Alternate gene names: 121998914

Gene position: 2335484-2334624 (Counterclockwise)

Preceding gene: 121998915

Following gene: 121998913

Centisome position: 87.2

GC content: 67.71

Gene sequence:

>861_bases
ATGCAACACAGCGCTAATCGAGCCAAAGGGCTCTCCGAGAATGGCCAGACCCGGCGCCACCGGGTGGCGGCGGTGCAGAT
GGCCTCGGGGCCGCATGTGGAGGCCAACCTCCAAGAGGCCGAACGGCTCATCGGCGAGGCTGCCGCAGCCGGGGCCGAGT
TGGTGGTCCTGCCCGAGAACTTCGCCATCATGGGCTATCAGGACACCGACAAGCTCAAGTACGCCGAACCCGACGGCGAT
GGCCCCATCCAGGCGTTCCTCGCCGAACAGGCGCGGCGCCACCGGATCGTGTTGGTCGGCGGCACCATCCCGCTGCAGGG
CGAGGGCCCCGAGCGCGTCCGGCCGACGGCCCCGGTCTATGGTCCGGACGGGGGCCGCCTGGCCTGTTACGATAAGATTC
ATCTATTCGATGTGGAGGTGGCTCCAGGTGAAGCCTATCGCGAGTCGAACACGCAGCAGCCCGGGGCGACCCCGCTGGTC
GTGGATACGCCCGTCGGACGGCTGGGGGTGGCGGTCTGCTACGATCTGCGCTTCCCGGAACTGTTCCGTGAGCTGGCCGA
CCAGGGTGCGGAGATCGTGGCCGTACCCTCGGCCTTCACCGCTGTCACCGGCGAGGCTCACTGGAGCATCCTGGTCCGCG
CCCGGGCCATCGAGAACCTCTTTTACATGGTGGCGCCGAATCAGGGTGGCTACCACGTCAACGGCCGCGAGACCCACGGT
GAGAGCATGGTGGTCGACCCCTGGGGGCGCGTCATGAAGAGCCTGGAACGCGGCGCCGGGGTGATCACCGTGGAGGTGAG
CCTGAGCCGCCTGCACGAGATCCGGGAGCGTTTCCCGGCACTCCTTCACCGCAAGCTGTAG

Upstream 100 bases:

>100_bases
GACCAATTGCTCGGTCAGGGTGTGGATCGGGCAGCGGCGGTGCGCTATCGCGTGGCGGGGCCGTGGCACGAGCCCCGCGT
CATGAGGCTTGGAGTCGAGG

Downstream 100 bases:

>100_bases
GTGACCGCATGAAGAATCACGCCGAAGCCGCTACTGCGACCCGGGACCCGCTCCACCACGCCGAGGCGCAGCTGCTGGCG
CCGGCCGCCCTCGATGAGGC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQHSANRAKGLSENGQTRRHRVAAVQMASGPHVEANLQEAERLIGEAAAAGAELVVLPENFAIMGYQDTDKLKYAEPDGD
GPIQAFLAEQARRHRIVLVGGTIPLQGEGPERVRPTAPVYGPDGGRLACYDKIHLFDVEVAPGEAYRESNTQQPGATPLV
VDTPVGRLGVAVCYDLRFPELFRELADQGAEIVAVPSAFTAVTGEAHWSILVRARAIENLFYMVAPNQGGYHVNGRETHG
ESMVVDPWGRVMKSLERGAGVITVEVSLSRLHEIRERFPALLHRKL

Sequences:

>Translated_286_residues
MQHSANRAKGLSENGQTRRHRVAAVQMASGPHVEANLQEAERLIGEAAAAGAELVVLPENFAIMGYQDTDKLKYAEPDGD
GPIQAFLAEQARRHRIVLVGGTIPLQGEGPERVRPTAPVYGPDGGRLACYDKIHLFDVEVAPGEAYRESNTQQPGATPLV
VDTPVGRLGVAVCYDLRFPELFRELADQGAEIVAVPSAFTAVTGEAHWSILVRARAIENLFYMVAPNQGGYHVNGRETHG
ESMVVDPWGRVMKSLERGAGVITVEVSLSRLHEIRERFPALLHRKL
>Mature_286_residues
MQHSANRAKGLSENGQTRRHRVAAVQMASGPHVEANLQEAERLIGEAAAAGAELVVLPENFAIMGYQDTDKLKYAEPDGD
GPIQAFLAEQARRHRIVLVGGTIPLQGEGPERVRPTAPVYGPDGGRLACYDKIHLFDVEVAPGEAYRESNTQQPGATPLV
VDTPVGRLGVAVCYDLRFPELFRELADQGAEIVAVPSAFTAVTGEAHWSILVRARAIENLFYMVAPNQGGYHVNGRETHG
ESMVVDPWGRVMKSLERGAGVITVEVSLSRLHEIRERFPALLHRKL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=269, Percent_Identity=39.0334572490706, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=39.0334572490706, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI5031947, Length=269, Percent_Identity=39.0334572490706, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=35.4716981132075, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=38.0208333333333, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786412, Length=249, Percent_Identity=30.1204819277108, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556280, Length=275, Percent_Identity=38.9090909090909, Blast_Score=180, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17533173, Length=265, Percent_Identity=27.5471698113208, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=34.3333333333333, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=35.1063829787234, Blast_Score=162, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21355835, Length=268, Percent_Identity=32.4626865671642, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI17933642, Length=279, Percent_Identity=35.84229390681, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21358471, Length=293, Percent_Identity=27.9863481228669, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31182; Mature: 31182

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHSANRAKGLSENGQTRRHRVAAVQMASGPHVEANLQEAERLIGEAAAAGAELVVLPEN
CCCCCCHHCCCCCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
FAIMGYQDTDKLKYAEPDGDGPIQAFLAEQARRHRIVLVGGTIPLQGEGPERVRPTAPVY
EEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCEE
GPDGGRLACYDKIHLFDVEVAPGEAYRESNTQQPGATPLVVDTPVGRLGVAVCYDLRFPE
CCCCCEEEEEEEEEEEEEEECCCHHHHCCCCCCCCCCEEEEECCCCHHEEEEEEECCCHH
LFRELADQGAEIVAVPSAFTAVTGEAHWSILVRARAIENLFYMVAPNQGGYHVNGRETHG
HHHHHHHCCCEEEECCCHHEEECCCCEEEEEEEHHHHCCEEEEECCCCCCEEECCCCCCC
ESMVVDPWGRVMKSLERGAGVITVEVSLSRLHEIRERFPALLHRKL
CEEEECHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQHSANRAKGLSENGQTRRHRVAAVQMASGPHVEANLQEAERLIGEAAAAGAELVVLPEN
CCCCCCHHCCCCCCCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
FAIMGYQDTDKLKYAEPDGDGPIQAFLAEQARRHRIVLVGGTIPLQGEGPERVRPTAPVY
EEEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCEEEEEECCEEECCCCCCCCCCCCCEE
GPDGGRLACYDKIHLFDVEVAPGEAYRESNTQQPGATPLVVDTPVGRLGVAVCYDLRFPE
CCCCCEEEEEEEEEEEEEEECCCHHHHCCCCCCCCCCEEEEECCCCHHEEEEEEECCCHH
LFRELADQGAEIVAVPSAFTAVTGEAHWSILVRARAIENLFYMVAPNQGGYHVNGRETHG
HHHHHHHCCCEEEECCCHHEEECCCCEEEEEEEHHHHCCEEEEECCCCCCEEECCCCCCC
ESMVVDPWGRVMKSLERGAGVITVEVSLSRLHEIRERFPALLHRKL
CEEEECHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]