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The map label for this gene is nudE

Identifier: 121998838

GI number: 121998838

Start: 2261336

End: 2261926

Strand: Reverse

Name: nudE

Synonym: Hhal_2059

Alternate gene names: 121998838

Gene position: 2261926-2261336 (Counterclockwise)

Preceding gene: 121998839

Following gene: 121998837

Centisome position: 84.45

GC content: 70.56

Gene sequence:

>591_bases
ATGTACAATGTCCGGACGCTGAGAAAGGCAGGCCCGATGAGCGATCGCTACCCGGAGACGCTCCACCGCAGCACCGTCGC
CCGCTCGCGGCTGTTCCGCATCGAGGCGGTGGGGCTGCGCTTTGGCAACGGCGTCGAGGTGGAGTACGAGCGCCTGGGCG
GGAGCGCAGCTGGGGCGGTGCTGGTCGTCCCGGTTACGGCCCGCGGCGAGGTGCTGCTCATCCGCGAGTACGCCGCCGGC
ACCGAGTGCTACGAACTGGGGCTGCCCAAGGGGCGCGTGGAACCCGGCGAGGATCCGCTGCGCGCCGCCAACCGCGAGCT
CATGGAGGAGGTCGGCTACGGCGCCGAGGCGCTCACCGTGCTGCGCAGTGTCACCCTGGCGCCGGCCTACTTCAGCCACC
GCACCCAGCTCATCCTCGCCGAGGGTCTCTTCGAGCACCGCCTGCCCGGCGACGAGCCGGAGCCCATCGAGGTGGTGCCC
TGGCCTCTGGGGGAACTGGAACGCCTCCTCGGCCAGTCCGATCTGACCGAGGCACGCTCGATCACCGCGCTTTATCTGGC
GCGGGATCATCTGGCAGGGCGCGCGGCGTAA

Upstream 100 bases:

>100_bases
TCAACGCCTGGGTCATCCCCACCGACGAGGAGCGGGTCATCGCCGCTCACGCCGCACGGCTGGTGCCCTGAAACGGCGCT
CGCCGCCGTCGGGGCGGCAT

Downstream 100 bases:

>100_bases
CCCGCGCCGGAACAACACGGAAGGGATCATGGCAGAGCAACCGTGGCAGCACTGGCTGGAGCCGGTGCGGGAGATCGCCG
AGCAGGCCGGCGAACGCATC

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MYNVRTLRKAGPMSDRYPETLHRSTVARSRLFRIEAVGLRFGNGVEVEYERLGGSAAGAVLVVPVTARGEVLLIREYAAG
TECYELGLPKGRVEPGEDPLRAANRELMEEVGYGAEALTVLRSVTLAPAYFSHRTQLILAEGLFEHRLPGDEPEPIEVVP
WPLGELERLLGQSDLTEARSITALYLARDHLAGRAA

Sequences:

>Translated_196_residues
MYNVRTLRKAGPMSDRYPETLHRSTVARSRLFRIEAVGLRFGNGVEVEYERLGGSAAGAVLVVPVTARGEVLLIREYAAG
TECYELGLPKGRVEPGEDPLRAANRELMEEVGYGAEALTVLRSVTLAPAYFSHRTQLILAEGLFEHRLPGDEPEPIEVVP
WPLGELERLLGQSDLTEARSITALYLARDHLAGRAA
>Mature_196_residues
MYNVRTLRKAGPMSDRYPETLHRSTVARSRLFRIEAVGLRFGNGVEVEYERLGGSAAGAVLVVPVTARGEVLLIREYAAG
TECYELGLPKGRVEPGEDPLRAANRELMEEVGYGAEALTVLRSVTLAPAYFSHRTQLILAEGLFEHRLPGDEPEPIEVVP
WPLGELERLLGQSDLTEARSITALYLARDHLAGRAA

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=177, Percent_Identity=49.1525423728814, Blast_Score=177, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21552; Mature: 21552

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNVRTLRKAGPMSDRYPETLHRSTVARSRLFRIEAVGLRFGNGVEVEYERLGGSAAGAV
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEHHHCCCCCCCEE
LVVPVTARGEVLLIREYAAGTECYELGLPKGRVEPGEDPLRAANRELMEEVGYGAEALTV
EEEEECCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LRSVTLAPAYFSHRTQLILAEGLFEHRLPGDEPEPIEVVPWPLGELERLLGQSDLTEARS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHCCCCHHHHHH
ITALYLARDHLAGRAA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYNVRTLRKAGPMSDRYPETLHRSTVARSRLFRIEAVGLRFGNGVEVEYERLGGSAAGAV
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCEEEHHHCCCCCCCEE
LVVPVTARGEVLLIREYAAGTECYELGLPKGRVEPGEDPLRAANRELMEEVGYGAEALTV
EEEEECCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LRSVTLAPAYFSHRTQLILAEGLFEHRLPGDEPEPIEVVPWPLGELERLLGQSDLTEARS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHCCCCHHHHHH
ITALYLARDHLAGRAA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]