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The map label for this gene is rlmI [H]

Identifier: 121998822

GI number: 121998822

Start: 2246108

End: 2247316

Strand: Reverse

Name: rlmI [H]

Synonym: Hhal_2043

Alternate gene names: 121998822

Gene position: 2247316-2246108 (Counterclockwise)

Preceding gene: 121998830

Following gene: 121998821

Centisome position: 83.9

GC content: 73.2

Gene sequence:

>1209_bases
ATGCAGCAACGACCCGCACTCCGCCTGAAACCCGGCGAGGAGCGCCGCCTGCGCGCCGGCCACCTGTGGATCTTCAGCAA
CGAGGTGGATACCGCCCACACCCCGCTGCGGGGGTTCGCGCCCGGCGAGCAGGCCGTGGTGGAGGACGCCCGCGGCAAGG
CCCTGGGGTGCGCCTACGTCAACCCCAACTCGCTGATCTGCGCCCGGCTGGTCAGTCGCGACGCCAAGGTGGCCCTGGAT
CGCTCGACCCTGGTCCACCGCCTGCAGGTGGCCCTGGCCGCACGCCAGCGCCTCTTCGCCGAGCCCTGGTACCGGCTGGT
CCACGGCGAGGCCGATGGCCTGCCCGGCCTGGTCATCGACCGTTTTGGGGACTGCTGTGTGGTGCAGCCGAACACCGCCG
GGATCGAGCGGTTGCAGGCCGAGGTGCTGGAGGCCCTGGAGAAGGTCGTCGCCCCGGCGTACGTCCTCTGGCGGGCCGAC
AACGCCGTGCGCGAGCGCGAGGGGCTGGACCTGCGCGTGGAGTGGCTCGGCCAGCCGGGGCCGGAGGAGCTGGAGGTCCG
CGAAGGGGGGCTGCACTTCCGGGTCCCGGTGGTCAGCGGGCAGAAGACGGGTTGGTTTTACGATCAGCAGGCCAATCGCC
GGCGCCTGGCCGCCTACGCCGGCGACGCCCGGGTGCTGGACGCCTTCTCCTACGCCGGCGGCTTCGCCATCGCCGCCGCG
GTGGCCGGCGCCCGCGAGGCGGTGGCCGTGGAGCGCTCCGCCGAGGCGTGTGACCGCATCGCCGCCAATGCCGAGCGCAA
CGGCGTCGGCGATCGGGTGACGGTGATCGAAGGCGAGGTCAACGACTACCTGGCGGCGGCCCGTCAGGAGGGCGAGCGCT
ACGACGTGGCGGTGGTGGATCCGCCGGCGTTCATCAAGCGCCGCCGCGACCGCAAGGCCGGTGAGCGCGGCTACCGCACG
GTCAACGAGGCGGCCCTGCGCCTGCTCGGCCGCGACGGCGTGCTGCTCAGCTGCTCCTGCTCGGCCCACCTGCCCGAGGA
GCGCCTCTCCGGCATCCTGCTGGCGGCCGGGCGGCACCTGGACCGCTCCGTGCGCATCCTCGAGCGCGGCGGTCTGCCGC
CGGACCACCCGATCCACCCGGCGATCCCCGAGACCGACTACCTCAAGGCGCTCTTCATCCGTGCGGTGATCGCCACCAGC
CTGCCGTGA

Upstream 100 bases:

>100_bases
CCCAACGATGGCGCTAGTCTAACAAGAAGCGCCCCTGGGGTCGCGATTGCGTGCGGCGCAGTGTCGCCGCAGAATACCGG
GCCCTGTCGAGGAGGCCTCC

Downstream 100 bases:

>100_bases
GGCGCCGGGGCCGCAGGACGCCGCGGGACCGGCAGCGGCCCTTGCGCGCCACCCCCGGAACCCGGAACACTGCGACACGA
CCCTGAGCCATCGACGCTGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MQQRPALRLKPGEERRLRAGHLWIFSNEVDTAHTPLRGFAPGEQAVVEDARGKALGCAYVNPNSLICARLVSRDAKVALD
RSTLVHRLQVALAARQRLFAEPWYRLVHGEADGLPGLVIDRFGDCCVVQPNTAGIERLQAEVLEALEKVVAPAYVLWRAD
NAVREREGLDLRVEWLGQPGPEELEVREGGLHFRVPVVSGQKTGWFYDQQANRRRLAAYAGDARVLDAFSYAGGFAIAAA
VAGAREAVAVERSAEACDRIAANAERNGVGDRVTVIEGEVNDYLAAARQEGERYDVAVVDPPAFIKRRRDRKAGERGYRT
VNEAALRLLGRDGVLLSCSCSAHLPEERLSGILLAAGRHLDRSVRILERGGLPPDHPIHPAIPETDYLKALFIRAVIATS
LP

Sequences:

>Translated_402_residues
MQQRPALRLKPGEERRLRAGHLWIFSNEVDTAHTPLRGFAPGEQAVVEDARGKALGCAYVNPNSLICARLVSRDAKVALD
RSTLVHRLQVALAARQRLFAEPWYRLVHGEADGLPGLVIDRFGDCCVVQPNTAGIERLQAEVLEALEKVVAPAYVLWRAD
NAVREREGLDLRVEWLGQPGPEELEVREGGLHFRVPVVSGQKTGWFYDQQANRRRLAAYAGDARVLDAFSYAGGFAIAAA
VAGAREAVAVERSAEACDRIAANAERNGVGDRVTVIEGEVNDYLAAARQEGERYDVAVVDPPAFIKRRRDRKAGERGYRT
VNEAALRLLGRDGVLLSCSCSAHLPEERLSGILLAAGRHLDRSVRILERGGLPPDHPIHPAIPETDYLKALFIRAVIATS
LP
>Mature_402_residues
MQQRPALRLKPGEERRLRAGHLWIFSNEVDTAHTPLRGFAPGEQAVVEDARGKALGCAYVNPNSLICARLVSRDAKVALD
RSTLVHRLQVALAARQRLFAEPWYRLVHGEADGLPGLVIDRFGDCCVVQPNTAGIERLQAEVLEALEKVVAPAYVLWRAD
NAVREREGLDLRVEWLGQPGPEELEVREGGLHFRVPVVSGQKTGWFYDQQANRRRLAAYAGDARVLDAFSYAGGFAIAAA
VAGAREAVAVERSAEACDRIAANAERNGVGDRVTVIEGEVNDYLAAARQEGERYDVAVVDPPAFIKRRRDRKAGERGYRT
VNEAALRLLGRDGVLLSCSCSAHLPEERLSGILLAAGRHLDRSVRILERGGLPPDHPIHPAIPETDYLKALFIRAVIATS
LP

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=34.3434343434343, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1787181, Length=246, Percent_Identity=28.8617886178862, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44111; Mature: 44111

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRPALRLKPGEERRLRAGHLWIFSNEVDTAHTPLRGFAPGEQAVVEDARGKALGCAYV
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEE
NPNSLICARLVSRDAKVALDRSTLVHRLQVALAARQRLFAEPWYRLVHGEADGLPGLVID
CCCHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RFGDCCVVQPNTAGIERLQAEVLEALEKVVAPAYVLWRADNAVREREGLDLRVEWLGQPG
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCEEEEEECCCCC
PEELEVREGGLHFRVPVVSGQKTGWFYDQQANRRRLAAYAGDARVLDAFSYAGGFAIAAA
CCCEEEECCCEEEEEEEECCCCCCCEECCCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHH
VAGAREAVAVERSAEACDRIAANAERNGVGDRVTVIEGEVNDYLAAARQEGERYDVAVVD
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEEC
PPAFIKRRRDRKAGERGYRTVNEAALRLLGRDGVLLSCSCSAHLPEERLSGILLAAGRHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCEEEEECCCCC
DRSVRILERGGLPPDHPIHPAIPETDYLKALFIRAVIATSLP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQRPALRLKPGEERRLRAGHLWIFSNEVDTAHTPLRGFAPGEQAVVEDARGKALGCAYV
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEE
NPNSLICARLVSRDAKVALDRSTLVHRLQVALAARQRLFAEPWYRLVHGEADGLPGLVID
CCCHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RFGDCCVVQPNTAGIERLQAEVLEALEKVVAPAYVLWRADNAVREREGLDLRVEWLGQPG
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCEEEEEECCCCC
PEELEVREGGLHFRVPVVSGQKTGWFYDQQANRRRLAAYAGDARVLDAFSYAGGFAIAAA
CCCEEEECCCEEEEEEEECCCCCCCEECCCCCCHHHHHHCCCHHHHHHHHHCCHHHHHHH
VAGAREAVAVERSAEACDRIAANAERNGVGDRVTVIEGEVNDYLAAARQEGERYDVAVVD
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEEC
PPAFIKRRRDRKAGERGYRTVNEAALRLLGRDGVLLSCSCSAHLPEERLSGILLAAGRHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCEEEEECCCCC
DRSVRILERGGLPPDHPIHPAIPETDYLKALFIRAVIATSLP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA