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The map label for this gene is purD [H]

Identifier: 121998778

GI number: 121998778

Start: 2203460

End: 2204749

Strand: Reverse

Name: purD [H]

Synonym: Hhal_1999

Alternate gene names: 121998778

Gene position: 2204749-2203460 (Counterclockwise)

Preceding gene: 121998779

Following gene: 121998772

Centisome position: 82.31

GC content: 71.01

Gene sequence:

>1290_bases
ATGAAGGTACTGGTGATCGGAGGGGGCGGCCGCGAGCACGCCATGGCCTGGGCCCTGGCGCGCTCCAAGCAGGTCGAGGA
GGTGCTGGTCGCCCCGGGGAATGCCGGCACGGAGCGCGAGCCCAAGGTGCGCAACATCCAGGTGGGGGCGGAGGATATCC
CCGCGCTGGTCCAACAGGCCCGCGAGCAGGAGGTGGCTTTGACCGTGGTCGGCCCCGAGGCGCCCCTGGTGGCCGGCGTG
GTGGACGCCTTCCAGCAGGCGGGTCTGGCGTGTCTGGGGCCGACGGCGGACGCCGCCGAGCTGGAGGGCTCGAAGGCGTT
TGCCAAGGCGTTCATGGCGCGTCACGGGGTGCCAACGGCCGCCTACCGCACCTTCGACGACCTGGGGGCGGCCAGCGACT
ATATCCGCGAGCACTCGACGCCGATGGTCATCAAGGCGGACGGCCTGGCCTCCGGCAAGGGGGTCGAGGTGGCGGCGACC
AAGGACGAGGCCCTATTGGCGGCCGAGCGCATGCTCTCGGGGCAGGCCTTCGGGGATGCCGGCGCGCGGGTCGTGGTCGA
GGAGTGCCTGCAGGGCGAGGAGCTGAGCTTCATCGCGCTGGTCGATGGCGAGCACGTGGTGGCGATGGCCAGCTCCCAGG
ATCACAAGCCGCGGGACGACGGAGATCGGGGCCCCAACACCGGTGGTATGGGGGCCTATTCGCCGGCGCCGCTGATGGAT
GAGCAGCTCTACCAGCGGGTCATGGACGAGGTGATCCGCCCCACGGTCCAGGGGCTGGCCGCCGAGGGGCGCCCCTATCA
GGGCTTCCTCTACGCCGGACTGATGATCGACGCCGACGGCAACCCCCGGGTGCTGGAGTACAACTGCCGCCTGGGTGACC
CGGAGGCGCAGCCGCTGTTGATGCGCCTGGACGCGGATTTTGCCGAAGTCTGCCGGGCCGCCCTCGAGGGGCGACTGGGC
GAGGTGGATCTGGCCTGGGACTCGCGTCCTGCTGTGGGCGTGGTGATGGCGGCGGCCGGCTATCCAGGGTCGGTGGAGCG
GGGCGATGTCATCGAAGGGCTCGACGACGCCGAGGCCACCGGCTGCAAGGTCTTCCACGGCGGCACGACCTTCGACGCCG
ACGGCCGTGTGGTGACCAACGGGGGGCGGGTGCTGTGCTGTTGCGCGCTGGGTGAGCGTGTCTCTGCAGCGCAGCAGGCG
GCCTACCGGGGGGTGGCGGCCATCCATTGGGAAGGGGTGTTCTACCGGCGGGATATCGGTGCGCGGGCCATCGCCCGGGA
GACCGGCTGA

Upstream 100 bases:

>100_bases
ATCAGGAGGTCATCGATGCCGCCGACGAGCACGGTCTGGCCATGGTCTTCACCGGGATGCGCCACTTCCGCCACTGAACG
CGGCCCAAGCGAGGTAAAGG

Downstream 100 bases:

>100_bases
CCGCGGGGTAGGTCTCGCCCTACGGGGCTGAAAACACAAAGGAGCCGGTGCCCCGCGGGGCACCGACTCCGTCACCGCGC
CTCCCCGAGGGGGTTACGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKVLVIGGGGREHAMAWALARSKQVEEVLVAPGNAGTEREPKVRNIQVGAEDIPALVQQAREQEVALTVVGPEAPLVAGV
VDAFQQAGLACLGPTADAAELEGSKAFAKAFMARHGVPTAAYRTFDDLGAASDYIREHSTPMVIKADGLASGKGVEVAAT
KDEALLAAERMLSGQAFGDAGARVVVEECLQGEELSFIALVDGEHVVAMASSQDHKPRDDGDRGPNTGGMGAYSPAPLMD
EQLYQRVMDEVIRPTVQGLAAEGRPYQGFLYAGLMIDADGNPRVLEYNCRLGDPEAQPLLMRLDADFAEVCRAALEGRLG
EVDLAWDSRPAVGVVMAAAGYPGSVERGDVIEGLDDAEATGCKVFHGGTTFDADGRVVTNGGRVLCCCALGERVSAAQQA
AYRGVAAIHWEGVFYRRDIGARAIARETG

Sequences:

>Translated_429_residues
MKVLVIGGGGREHAMAWALARSKQVEEVLVAPGNAGTEREPKVRNIQVGAEDIPALVQQAREQEVALTVVGPEAPLVAGV
VDAFQQAGLACLGPTADAAELEGSKAFAKAFMARHGVPTAAYRTFDDLGAASDYIREHSTPMVIKADGLASGKGVEVAAT
KDEALLAAERMLSGQAFGDAGARVVVEECLQGEELSFIALVDGEHVVAMASSQDHKPRDDGDRGPNTGGMGAYSPAPLMD
EQLYQRVMDEVIRPTVQGLAAEGRPYQGFLYAGLMIDADGNPRVLEYNCRLGDPEAQPLLMRLDADFAEVCRAALEGRLG
EVDLAWDSRPAVGVVMAAAGYPGSVERGDVIEGLDDAEATGCKVFHGGTTFDADGRVVTNGGRVLCCCALGERVSAAQQA
AYRGVAAIHWEGVFYRRDIGARAIARETG
>Mature_429_residues
MKVLVIGGGGREHAMAWALARSKQVEEVLVAPGNAGTEREPKVRNIQVGAEDIPALVQQAREQEVALTVVGPEAPLVAGV
VDAFQQAGLACLGPTADAAELEGSKAFAKAFMARHGVPTAAYRTFDDLGAASDYIREHSTPMVIKADGLASGKGVEVAAT
KDEALLAAERMLSGQAFGDAGARVVVEECLQGEELSFIALVDGEHVVAMASSQDHKPRDDGDRGPNTGGMGAYSPAPLMD
EQLYQRVMDEVIRPTVQGLAAEGRPYQGFLYAGLMIDADGNPRVLEYNCRLGDPEAQPLLMRLDADFAEVCRAALEGRLG
EVDLAWDSRPAVGVVMAAAGYPGSVERGDVIEGLDDAEATGCKVFHGGTTFDADGRVVTNGGRVLCCCALGERVSAAQQA
AYRGVAAIHWEGVFYRRDIGARAIARETG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=48.8262910798122, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=48.8262910798122, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=48.8262910798122, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=48.8262910798122, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=59.9531615925059, Blast_Score=521, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=38.9671361502347, Blast_Score=288, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=39.6355353075171, Blast_Score=324, Evalue=2e-89,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.8837209302326, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.8837209302326, Blast_Score=330, Evalue=9e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45136; Mature: 45136

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGGGREHAMAWALARSKQVEEVLVAPGNAGTEREPKVRNIQVGAEDIPALVQQA
CEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHH
REQEVALTVVGPEAPLVAGVVDAFQQAGLACLGPTADAAELEGSKAFAKAFMARHGVPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCHH
AYRTFDDLGAASDYIREHSTPMVIKADGLASGKGVEVAATKDEALLAAERMLSGQAFGDA
HHHHHHHHCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCC
GARVVVEECLQGEELSFIALVDGEHVVAMASSQDHKPRDDGDRGPNTGGMGAYSPAPLMD
CHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
EQLYQRVMDEVIRPTVQGLAAEGRPYQGFLYAGLMIDADGNPRVLEYNCRLGDPEAQPLL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCCEE
MRLDADFAEVCRAALEGRLGEVDLAWDSRPAVGVVMAAAGYPGSVERGDVIEGLDDAEAT
EEECCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHCCCCCCCCC
GCKVFHGGTTFDADGRVVTNGGRVLCCCALGERVSAAQQAAYRGVAAIHWEGVFYRRDIG
CEEEEECCEEECCCCEEEECCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCEEEECCCC
ARAIARETG
CHHHHCCCC
>Mature Secondary Structure
MKVLVIGGGGREHAMAWALARSKQVEEVLVAPGNAGTEREPKVRNIQVGAEDIPALVQQA
CEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHH
REQEVALTVVGPEAPLVAGVVDAFQQAGLACLGPTADAAELEGSKAFAKAFMARHGVPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCHH
AYRTFDDLGAASDYIREHSTPMVIKADGLASGKGVEVAATKDEALLAAERMLSGQAFGDA
HHHHHHHHCHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCC
GARVVVEECLQGEELSFIALVDGEHVVAMASSQDHKPRDDGDRGPNTGGMGAYSPAPLMD
CHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
EQLYQRVMDEVIRPTVQGLAAEGRPYQGFLYAGLMIDADGNPRVLEYNCRLGDPEAQPLL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCCEE
MRLDADFAEVCRAALEGRLGEVDLAWDSRPAVGVVMAAAGYPGSVERGDVIEGLDDAEAT
EEECCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHCCCCCCCCC
GCKVFHGGTTFDADGRVVTNGGRVLCCCALGERVSAAQQAAYRGVAAIHWEGVFYRRDIG
CEEEEECCEEECCCCEEEECCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCEEEECCCC
ARAIARETG
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]