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The map label for this gene is purH [H]

Identifier: 121998779

GI number: 121998779

Start: 2204773

End: 2206356

Strand: Reverse

Name: purH [H]

Synonym: Hhal_2000

Alternate gene names: 121998779

Gene position: 2206356-2204773 (Counterclockwise)

Preceding gene: 121998780

Following gene: 121998778

Centisome position: 82.37

GC content: 69.44

Gene sequence:

>1584_bases
ATGGCGACCAACGACGGCGTGCGGCCCCTGCGGCGGGCCCTGATCAGCGTTTCCGATAAGAGCGGGGTGGAGGGCTTTGC
CCGCGCCCTGCATGAGCAAGGCGTCGAGATCCTCTCGACCGGTGGTACGGCCCGCCTGCTGGGTGAGGCCGGGATCCCGG
TGCGGGAGGTCTCGGCCGAGACCGGCTTCCCGGAGATCATGGACGGTCGCGTCAAGACCCTGCATCCGCGCATCCACGGC
GGGCTGCTGGGCCGGCGCGGCACGGATGACGCGGTCATGGACGAGCACGGCATTGGTCCCATCGATCTGCTTTGCGTCAA
CCTCTACCCCTTCGAGCAGGCCGTGGCCGCCGAGGGCTGCACCTTGACCGACGCCATCGAGAATATCGATGTCGGCGGGC
CGGCGATGATCCGTGCGGCCGCCAAGAACCACGCCGACGTGGCGGTGGTGACCGAGTCGTCGGCCTATGGCCTGGTCCTC
GATGAGCTCCAGCGCCTTGGCGGGACCAGCCGCGCCCTGCGCCACCATCTGGCGACCCGAGCGTTCAGCCACACCGCGCG
CTACGACGGCGCCATCGCTGCCTACCTGAGCCAGCGCGACGAACAGGGCGAGCAGCAGGGCGATTTCCCGGCGATCTGGA
CGCTCCAGGTGGAGAAGGTCGCCGACATGCGTTACGGCGAGAACCCCCATCAATCCGCTGCCTTCTATCGCGATGTCGCT
CCCGGCGAGGCCAGCGTGTCCACCGCCCGCCAGCTCCAGGGTAAGGCCCTGTCGTACAACAACGTGGCCGACACCGACGC
CGCCCTGGAGTGCGTCAAGGGCTTCCAGACGCCGGCCTGCGTCATCGTCAAGCACGCCAATCCCTGCGGCGTGGCCTGCT
CGGGGACGTTGCGGGAGGCCTACGACCGGGCCTTCGAGGTCGATCCGACCTCCGCCTTCGGCGGCATCATCGCCTTCAAC
GATACCGTCGACGCCGAGCTGGCCGGCGCCATCCTCGATCGCCAGTTCGTCGAGGTGGTCATCGCCCCGGAGGTCAGTGA
CGAAGCCCTGTCGCGCTTCGCCGCCAAGGCCAACGTGCGAGTCCTGCAGACCGGCCGCTGGCCGCAGCATCCGGGCGCGG
ATCTGGAGCTCAAGCGGGTGCGTGGCGGCCTCCTGGTGCAGGACCGGGACACCGCGGTGGTTGATCCGGCCGACCTGCGG
GTGGTCACCAAGCGCCAGCCCACCGATGCCGAGTGGGCCGACCTGCGCTTCGCCTGGGAGGTGGTGCGGCACGTGAAGTC
CAACGCCATCGTTTTCGCCGGCGGGCAGCGCACCCTCGGCGTGGGGGCCGGGCAGATGAGCCGTGTCTTCAGTACCCGTA
TTGCCTGCGAGAAGGCGGCCGATGCGGGCCTGGCGCTGCAGGGCTCGGTCCTGGCCTCTGACGCCTTCTTCCCGTTCCGC
GACGGCGTCGATCAGGCTGCCGAGGCCGGCGCCGCCGCCGTGATCCAGCCCGGTGGCTCGATGCGGGATCAGGAGGTCAT
CGATGCCGCCGACGAGCACGGTCTGGCCATGGTCTTCACCGGGATGCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
CGCGGGCGGCTCAGCTGCTCGGCCTCAATCGAGCCACGCTGCGCAAGAAGCTCCGTGAACACGGCATCGACTACAACTGA
ACCAACCCGGACAATCCAAT

Downstream 100 bases:

>100_bases
ACGCGGCCCAAGCGAGGTAAAGGATGAAGGTACTGGTGATCGGAGGGGGCGGCCGCGAGCACGCCATGGCCTGGGCCCTG
GCGCGCTCCAAGCAGGTCGA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MATNDGVRPLRRALISVSDKSGVEGFARALHEQGVEILSTGGTARLLGEAGIPVREVSAETGFPEIMDGRVKTLHPRIHG
GLLGRRGTDDAVMDEHGIGPIDLLCVNLYPFEQAVAAEGCTLTDAIENIDVGGPAMIRAAAKNHADVAVVTESSAYGLVL
DELQRLGGTSRALRHHLATRAFSHTARYDGAIAAYLSQRDEQGEQQGDFPAIWTLQVEKVADMRYGENPHQSAAFYRDVA
PGEASVSTARQLQGKALSYNNVADTDAALECVKGFQTPACVIVKHANPCGVACSGTLREAYDRAFEVDPTSAFGGIIAFN
DTVDAELAGAILDRQFVEVVIAPEVSDEALSRFAAKANVRVLQTGRWPQHPGADLELKRVRGGLLVQDRDTAVVDPADLR
VVTKRQPTDAEWADLRFAWEVVRHVKSNAIVFAGGQRTLGVGAGQMSRVFSTRIACEKAADAGLALQGSVLASDAFFPFR
DGVDQAAEAGAAAVIQPGGSMRDQEVIDAADEHGLAMVFTGMRHFRH

Sequences:

>Translated_527_residues
MATNDGVRPLRRALISVSDKSGVEGFARALHEQGVEILSTGGTARLLGEAGIPVREVSAETGFPEIMDGRVKTLHPRIHG
GLLGRRGTDDAVMDEHGIGPIDLLCVNLYPFEQAVAAEGCTLTDAIENIDVGGPAMIRAAAKNHADVAVVTESSAYGLVL
DELQRLGGTSRALRHHLATRAFSHTARYDGAIAAYLSQRDEQGEQQGDFPAIWTLQVEKVADMRYGENPHQSAAFYRDVA
PGEASVSTARQLQGKALSYNNVADTDAALECVKGFQTPACVIVKHANPCGVACSGTLREAYDRAFEVDPTSAFGGIIAFN
DTVDAELAGAILDRQFVEVVIAPEVSDEALSRFAAKANVRVLQTGRWPQHPGADLELKRVRGGLLVQDRDTAVVDPADLR
VVTKRQPTDAEWADLRFAWEVVRHVKSNAIVFAGGQRTLGVGAGQMSRVFSTRIACEKAADAGLALQGSVLASDAFFPFR
DGVDQAAEAGAAAVIQPGGSMRDQEVIDAADEHGLAMVFTGMRHFRH
>Mature_526_residues
ATNDGVRPLRRALISVSDKSGVEGFARALHEQGVEILSTGGTARLLGEAGIPVREVSAETGFPEIMDGRVKTLHPRIHGG
LLGRRGTDDAVMDEHGIGPIDLLCVNLYPFEQAVAAEGCTLTDAIENIDVGGPAMIRAAAKNHADVAVVTESSAYGLVLD
ELQRLGGTSRALRHHLATRAFSHTARYDGAIAAYLSQRDEQGEQQGDFPAIWTLQVEKVADMRYGENPHQSAAFYRDVAP
GEASVSTARQLQGKALSYNNVADTDAALECVKGFQTPACVIVKHANPCGVACSGTLREAYDRAFEVDPTSAFGGIIAFND
TVDAELAGAILDRQFVEVVIAPEVSDEALSRFAAKANVRVLQTGRWPQHPGADLELKRVRGGLLVQDRDTAVVDPADLRV
VTKRQPTDAEWADLRFAWEVVRHVKSNAIVFAGGQRTLGVGAGQMSRVFSTRIACEKAADAGLALQGSVLASDAFFPFRD
GVDQAAEAGAAAVIQPGGSMRDQEVIDAADEHGLAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=530, Percent_Identity=35.2830188679245, Blast_Score=251, Evalue=8e-67,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=63.3776091081594, Blast_Score=665, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=519, Percent_Identity=31.4065510597302, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=355, Percent_Identity=25.9154929577465, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=38.655462184874, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=35.1351351351351, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=528, Percent_Identity=32.1969696969697, Blast_Score=256, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24649832, Length=509, Percent_Identity=36.1493123772102, Blast_Score=249, Evalue=5e-66,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56269; Mature: 56138

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNDGVRPLRRALISVSDKSGVEGFARALHEQGVEILSTGGTARLLGEAGIPVREVSAE
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHCCCCC
TGFPEIMDGRVKTLHPRIHGGLLGRRGTDDAVMDEHGIGPIDLLCVNLYPFEQAVAAEGC
CCCHHHHCCCCEEECCHHHCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCHHHHHHHCCC
TLTDAIENIDVGGPAMIRAAAKNHADVAVVTESSAYGLVLDELQRLGGTSRALRHHLATR
CHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
AFSHTARYDGAIAAYLSQRDEQGEQQGDFPAIWTLQVEKVADMRYGENPHQSAAFYRDVA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHCCC
PGEASVSTARQLQGKALSYNNVADTDAALECVKGFQTPACVIVKHANPCGVACSGTLREA
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECCCHHHHH
YDRAFEVDPTSAFGGIIAFNDTVDAELAGAILDRQFVEVVIAPEVSDEALSRFAAKANVR
HHHCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCEE
VLQTGRWPQHPGADLELKRVRGGLLVQDRDTAVVDPADLRVVTKRQPTDAEWADLRFAWE
EEECCCCCCCCCCCCCHHCCCCCEEEECCCCEEECCCCCEEEECCCCCCCHHHHHHHHHH
VVRHVKSNAIVFAGGQRTLGVGAGQMSRVFSTRIACEKAADAGLALQGSVLASDAFFPFR
HHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCCC
DGVDQAAEAGAAAVIQPGGSMRDQEVIDAADEHGLAMVFTGMRHFRH
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHCC
>Mature Secondary Structure 
ATNDGVRPLRRALISVSDKSGVEGFARALHEQGVEILSTGGTARLLGEAGIPVREVSAE
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHCCCCC
TGFPEIMDGRVKTLHPRIHGGLLGRRGTDDAVMDEHGIGPIDLLCVNLYPFEQAVAAEGC
CCCHHHHCCCCEEECCHHHCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCHHHHHHHCCC
TLTDAIENIDVGGPAMIRAAAKNHADVAVVTESSAYGLVLDELQRLGGTSRALRHHLATR
CHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
AFSHTARYDGAIAAYLSQRDEQGEQQGDFPAIWTLQVEKVADMRYGENPHQSAAFYRDVA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHCCC
PGEASVSTARQLQGKALSYNNVADTDAALECVKGFQTPACVIVKHANPCGVACSGTLREA
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECCCHHHHH
YDRAFEVDPTSAFGGIIAFNDTVDAELAGAILDRQFVEVVIAPEVSDEALSRFAAKANVR
HHHCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCEE
VLQTGRWPQHPGADLELKRVRGGLLVQDRDTAVVDPADLRVVTKRQPTDAEWADLRFAWE
EEECCCCCCCCCCCCCHHCCCCCEEEECCCCEEECCCCCEEEECCCCCCCHHHHHHHHHH
VVRHVKSNAIVFAGGQRTLGVGAGQMSRVFSTRIACEKAADAGLALQGSVLASDAFFPFR
HHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCCC
DGVDQAAEAGAAAVIQPGGSMRDQEVIDAADEHGLAMVFTGMRHFRH
CCHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA