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The map label for this gene is btuF [H]

Identifier: 121998702

GI number: 121998702

Start: 2118360

End: 2119214

Strand: Reverse

Name: btuF [H]

Synonym: Hhal_1923

Alternate gene names: 121998702

Gene position: 2119214-2118360 (Counterclockwise)

Preceding gene: 121998703

Following gene: 121998701

Centisome position: 79.12

GC content: 69.24

Gene sequence:

>855_bases
GTGCTGCTGGCGGCGTGGTCGCCGGTGCATGCTGGTGAACGCTGCGTGGAGGACGACGCCGGCCGCGAGGTCTGCCTGGA
GGCCCCGGCGGAGCGGATTGTGGCCCTCTCGCCCGGGGCGACGGAACTGCTCTTCGCCGCCGGCGCCGGGGACCATGTGG
TGGCCGCGGTCAGTCACGCGGATTATCCGGAGGCGGCGGAGGAACTGCCCCGGGTCGGCACCTACGACCGGCTCGATATG
GAGTCGATCCTCGCGAAGGATCCGGATCTGGTGGTGGGCTGGACCAGCGGCAACTCCAGCAGCCAGCTGGAGCGGATCGA
GGCGCTGGGCCTGCCGCTCTACCGCAGCGATCCGAAGGTCCTCGACGAGATCGCCACCTCCCTGGAGCGTCTGGGTACCC
TGGCCGGCACTGAAGACGAGGCCGACGCCGAGGCCGCCCGCTTCCGCGATGCGCGGGACGAGCTGGAGGCCGAGTACGCC
GACCGCGATCCGGTGCGGGTCTTCTACCAGATCTGGCCGGACCCGGTGATGACCGTCAACGACGAGCAGATCATCAGCGA
GTCGATCCGCATCTGCGGCGGCGAGAACGTCTTCGGTGATCTCGACAGCCTGACCCCGCGGCTGGACACCGAGTCCATCC
TTGCGGCCGACCCGGAGGCGATCGTCGCCGGCGGCATGGGCGAGGCGGATGACTCGTGGCTGGAGGAGTGGCGGCGCTAT
GACATCCTCGAGGCCACCGCCAAGGACAACCTCTTTTTTGTCGAGCCGTCCACGATCCAGCGCCCGACGCCACGCATCCT
GGAGGGGACGCGGGTGCTGTGTGACAAGCTCCAGCAGGCCCGGCGCAATCGCTGA

Upstream 100 bases:

>100_bases
TGCTGGCCAGCCTGGAGGCCCGGCTCATCGGGGCCGAGGTCTTCGAGGACGCCCCGCCGGGGCCCGAACCGCGCTAGGGG
GCATCGCAGCCGGATTTCTG

Downstream 100 bases:

>100_bases
TGGGGGTTGAGCGCACAGCCGATCAGCGGGCGCGCGCCGGCGCGGTGAGTGAGGAGGGCGGCGCTTTGGCCCAGCCGAGT
CGCATCGGGTCGCCGGTGCG

Product: periplasmic-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLLAAWSPVHAGERCVEDDAGREVCLEAPAERIVALSPGATELLFAAGAGDHVVAAVSHADYPEAAEELPRVGTYDRLDM
ESILAKDPDLVVGWTSGNSSSQLERIEALGLPLYRSDPKVLDEIATSLERLGTLAGTEDEADAEAARFRDARDELEAEYA
DRDPVRVFYQIWPDPVMTVNDEQIISESIRICGGENVFGDLDSLTPRLDTESILAADPEAIVAGGMGEADDSWLEEWRRY
DILEATAKDNLFFVEPSTIQRPTPRILEGTRVLCDKLQQARRNR

Sequences:

>Translated_284_residues
MLLAAWSPVHAGERCVEDDAGREVCLEAPAERIVALSPGATELLFAAGAGDHVVAAVSHADYPEAAEELPRVGTYDRLDM
ESILAKDPDLVVGWTSGNSSSQLERIEALGLPLYRSDPKVLDEIATSLERLGTLAGTEDEADAEAARFRDARDELEAEYA
DRDPVRVFYQIWPDPVMTVNDEQIISESIRICGGENVFGDLDSLTPRLDTESILAADPEAIVAGGMGEADDSWLEEWRRY
DILEATAKDNLFFVEPSTIQRPTPRILEGTRVLCDKLQQARRNR
>Mature_284_residues
MLLAAWSPVHAGERCVEDDAGREVCLEAPAERIVALSPGATELLFAAGAGDHVVAAVSHADYPEAAEELPRVGTYDRLDM
ESILAKDPDLVVGWTSGNSSSQLERIEALGLPLYRSDPKVLDEIATSLERLGTLAGTEDEADAEAARFRDARDELEAEYA
DRDPVRVFYQIWPDPVMTVNDEQIISESIRICGGENVFGDLDSLTPRLDTESILAADPEAIVAGGMGEADDSWLEEWRRY
DILEATAKDNLFFVEPSTIQRPTPRILEGTRVLCDKLQQARRNR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=32.4, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 31240; Mature: 31240

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAAWSPVHAGERCVEDDAGREVCLEAPAERIVALSPGATELLFAAGAGDHVVAAVSHA
CEEECCCCCCCCHHHHHCCCCCHHHHHCCHHHEEEECCCCCEEEEECCCCCCEEEEECCC
DYPEAAEELPRVGTYDRLDMESILAKDPDLVVGWTSGNSSSQLERIEALGLPLYRSDPKV
CCHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHH
LDEIATSLERLGTLAGTEDEADAEAARFRDARDELEAEYADRDPVRVFYQIWPDPVMTVN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
DEQIISESIRICGGENVFGDLDSLTPRLDTESILAADPEAIVAGGMGEADDSWLEEWRRY
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHH
DILEATAKDNLFFVEPSTIQRPTPRILEGTRVLCDKLQQARRNR
HHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLAAWSPVHAGERCVEDDAGREVCLEAPAERIVALSPGATELLFAAGAGDHVVAAVSHA
CEEECCCCCCCCHHHHHCCCCCHHHHHCCHHHEEEECCCCCEEEEECCCCCCEEEEECCC
DYPEAAEELPRVGTYDRLDMESILAKDPDLVVGWTSGNSSSQLERIEALGLPLYRSDPKV
CCHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHH
LDEIATSLERLGTLAGTEDEADAEAARFRDARDELEAEYADRDPVRVFYQIWPDPVMTVN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
DEQIISESIRICGGENVFGDLDSLTPRLDTESILAADPEAIVAGGMGEADDSWLEEWRRY
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHH
DILEATAKDNLFFVEPSTIQRPTPRILEGTRVLCDKLQQARRNR
HHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]