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The map label for this gene is leuD [H]

Identifier: 121998590

GI number: 121998590

Start: 1984218

End: 1984940

Strand: Reverse

Name: leuD [H]

Synonym: Hhal_1811

Alternate gene names: 121998590

Gene position: 1984940-1984218 (Counterclockwise)

Preceding gene: 121998591

Following gene: 121998589

Centisome position: 74.11

GC content: 63.9

Gene sequence:

>723_bases
ATGGTCACTTCGTGGATATCCGGGAGTAGACGCCGAGCGTCTCCCGGCCCTTTTTTCAAAAGACTGGAAACGCCCATGGA
ACCTTTCACTCGACACACCGGTCTGGTTGCGCCGCTGGATCGCTCCAACGTCGATACGGACGCCATCATCCCCAAGCAGT
TCCTCAAGTCCGTCAAGCGGACGGGGTTCGGCCCCTACCTGTTCGATGAGTGGCGGTACCTTGATCGCGGCGAGCCGGGG
ATGGACTGCACGAACCGGCCGCGCAACGAGGACTTCGTCCTCAATCAGCCGCGCTACCAGGGGGCCAGCGTGCTGCTTGC
CCGGGACAATTTCGGCTGCGGCTCCTCCCGCGAGCACGCCCCGTGGGCCCTGAAGGACTACGGCTTCCGGGCCATCGTGG
CGCCGAGCTTCGCCGATATCTTCTACAGCAACTGCTTCAAGAACGGCCTCCTGCCGGTGGTGCTGGGCGAGGAGGTGGTC
GAGCAGCTGTTTCAGGCGGTCTACGCCACCGAGGGCTACCAGCTGACCATCGATCTCTTCGAGCAGCAGGTCGTCACCCC
GGGCGGCGAGCGTTTCGGCTTCGACGTGGATCCGTTCCGCAAGCGCATGCTGCTTGAAGGCCTCGATGAGATCGGCGTGA
CCCTGGAGGAGCAGAGCGAGGCCATCCAGGCCTATGAGCAGCGCCGCCTGCAGGAAGCCCCCTGGCTGTTCCTTGAGCGC
TGA

Upstream 100 bases:

>100_bases
CGCTGTGCCTCGACCTCCAACCGGAACTTCGAGGGGCGCCAGGGCCAGGGCGGCCGCACCCATCTGGCCAGCCCGGCGAT
GGTGGCCGCTGCCGCCATCC

Downstream 100 bases:

>100_bases
ACCCGGAAACCTGAAATCTGAATGGTGAGTGCGTAACGATGGCCCAGAAGATCCTGCTGCTCCCCGGGGACGGTATCGGC
CCGGAGATCACCGCGGAGGC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVTSWISGSRRRASPGPFFKRLETPMEPFTRHTGLVAPLDRSNVDTDAIIPKQFLKSVKRTGFGPYLFDEWRYLDRGEPG
MDCTNRPRNEDFVLNQPRYQGASVLLARDNFGCGSSREHAPWALKDYGFRAIVAPSFADIFYSNCFKNGLLPVVLGEEVV
EQLFQAVYATEGYQLTIDLFEQQVVTPGGERFGFDVDPFRKRMLLEGLDEIGVTLEEQSEAIQAYEQRRLQEAPWLFLER

Sequences:

>Translated_240_residues
MVTSWISGSRRRASPGPFFKRLETPMEPFTRHTGLVAPLDRSNVDTDAIIPKQFLKSVKRTGFGPYLFDEWRYLDRGEPG
MDCTNRPRNEDFVLNQPRYQGASVLLARDNFGCGSSREHAPWALKDYGFRAIVAPSFADIFYSNCFKNGLLPVVLGEEVV
EQLFQAVYATEGYQLTIDLFEQQVVTPGGERFGFDVDPFRKRMLLEGLDEIGVTLEEQSEAIQAYEQRRLQEAPWLFLER
>Mature_240_residues
MVTSWISGSRRRASPGPFFKRLETPMEPFTRHTGLVAPLDRSNVDTDAIIPKQFLKSVKRTGFGPYLFDEWRYLDRGEPG
MDCTNRPRNEDFVLNQPRYQGASVLLARDNFGCGSSREHAPWALKDYGFRAIVAPSFADIFYSNCFKNGLLPVVLGEEVV
EQLFQAVYATEGYQLTIDLFEQQVVTPGGERFGFDVDPFRKRMLLEGLDEIGVTLEEQSEAIQAYEQRRLQEAPWLFLER

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=203, Percent_Identity=57.6354679802956, Blast_Score=234, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321429, Length=217, Percent_Identity=47.4654377880184, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 27488; Mature: 27488

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSWISGSRRRASPGPFFKRLETPMEPFTRHTGLVAPLDRSNVDTDAIIPKQFLKSVKR
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHH
TGFGPYLFDEWRYLDRGEPGMDCTNRPRNEDFVLNQPRYQGASVLLARDNFGCGSSREHA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCC
PWALKDYGFRAIVAPSFADIFYSNCFKNGLLPVVLGEEVVEQLFQAVYATEGYQLTIDLF
CEEHHHCCCEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCEEEHEEH
EQQVVTPGGERFGFDVDPFRKRMLLEGLDEIGVTLEEQSEAIQAYEQRRLQEAPWLFLER
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MVTSWISGSRRRASPGPFFKRLETPMEPFTRHTGLVAPLDRSNVDTDAIIPKQFLKSVKR
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHCCHHHHHHHHHH
TGFGPYLFDEWRYLDRGEPGMDCTNRPRNEDFVLNQPRYQGASVLLARDNFGCGSSREHA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCC
PWALKDYGFRAIVAPSFADIFYSNCFKNGLLPVVLGEEVVEQLFQAVYATEGYQLTIDLF
CEEHHHCCCEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCEEEHEEH
EQQVVTPGGERFGFDVDPFRKRMLLEGLDEIGVTLEEQSEAIQAYEQRRLQEAPWLFLER
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA