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The map label for this gene is leuC

Identifier: 121998591

GI number: 121998591

Start: 1984912

End: 1986312

Strand: Reverse

Name: leuC

Synonym: Hhal_1812

Alternate gene names: 121998591

Gene position: 1986312-1984912 (Counterclockwise)

Preceding gene: 121998592

Following gene: 121998590

Centisome position: 74.16

GC content: 67.67

Gene sequence:

>1401_bases
ATGACCGGGAAGACCCTCTACGACAAACTCTGGGATAGCCACGTTGTCACCGAGTACGACGATGGATCGGCGCTGCTTTA
CATCGATCGTCAGCTCCTGCATGAGGTGACCTCGCCGCAGGCGTTCGAGGGTCTGCGCCTGGCCGGTCGCCAGCCGTGGC
GCGTGGCGTCCAATCTTGCCGTGACCGATCACAACGTCCCCACCACCGACCGCAGTCAGCCGGTGGAGGATCCGGTCTCG
CGGGTGCAGATCGAGACGCTGGATCGCAACTGCAAGGACTTCCAGGTGATCGAGTTTGGTATCCGCGACCCGCGCCAAGG
GATCGTCCACGTCGTCGGGCCTGAGCAGGGCACGACACTGCCGGGGATGACCCTGGTCTGTGGCGACTCGCACACCTCGA
CCCACGGCGCCCTGGGCGCACTGGCCTTCGGGGTGGGCACCAGTGAGGTGGAGCATGTCCTGGCCACGCAGACCCTGGTG
CAGAAGAAGGCCCGCACCATGCTCATCCGCATCGATGGTCAGCTCGGGCGGGGGGTCACGGCCAAGGACATCATCCTGGC
GATCATCGGTCGCATCGGTACTGCGGGCGGCACGGGGTACGCACTGGAGTACGGCGGCGAGGCCATACGCAGCCTCTCCA
TGGAAGGGCGGATGACCATCTGCAACATGTCCATCGAGGCCGGGGCGCGTACCGGTATGGTGGCTGTGGATGACACCACC
ATCGAGTATGTCCGGGGCCGTCCGAATGCCCCCGAGGGCGCGCTGTGGGACCAGGCCGTGGCCAGTTGGCGCCACCTGGT
CTCGGATGAGGATGCGGCCTTCGACCGGGTGGTGGAACTCCACGCCGACGAGATCGAGCCCCAGGTGACCTGGGGGACGT
CGCCGGAGATGGTCGCCTCGGTGAATCGCCGCGTCCCCGACCCGGCGGAGGAGAGCGATGCGGTGCGCGCCCGGGCGATG
GGCCGGGCCCTGGAGTACATGGGCCTGGAGCCGGGGACGCCACTGACCGATATCCCCATGGACAAGATCTTCATCGGGTC
TTGCACCAATGCCCGCATCGAGGACCTGCGCGAGGCGGCCGCCGTCGTCCATGGCCGTCGGGTGGCTGAGAATATCCGCC
AGGCGCTGGTGGTCCCCGGCTCCGGGGTGGTCAAGCAGCAGGCCGAAGGCGAGGGGCTCGACCGGGTCTTTCTCGATGCC
GGCTTCGAGTGGCGCGAACCGGGGTGCTCCATGTGCCTGGGCATGAACCCCGACCGCCTGGAGCCCGGCGAGCGCTGTGC
CTCGACCTCCAACCGGAACTTCGAGGGGCGCCAGGGCCAGGGCGGCCGCACCCATCTGGCCAGCCCGGCGATGGTGGCCG
CTGCCGCCATCCATGGTCACTTCGTGGATATCCGGGAGTAG

Upstream 100 bases:

>100_bases
GCCTGCGCGGGCAGCGGGCCTGAGGGCATTTCCCCCGAGGGGGGTCGGTCCGATATAATGTTCAAGTGAATGTTTCTAAG
CCAATTGATAGAACAGGCCA

Downstream 100 bases:

>100_bases
ACGCCGAGCGTCTCCCGGCCCTTTTTTCAAAAGACTGGAAACGCCCATGGAACCTTTCACTCGACACACCGGTCTGGTTG
CGCCGCTGGATCGCTCCAAC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTGKTLYDKLWDSHVVTEYDDGSALLYIDRQLLHEVTSPQAFEGLRLAGRQPWRVASNLAVTDHNVPTTDRSQPVEDPVS
RVQIETLDRNCKDFQVIEFGIRDPRQGIVHVVGPEQGTTLPGMTLVCGDSHTSTHGALGALAFGVGTSEVEHVLATQTLV
QKKARTMLIRIDGQLGRGVTAKDIILAIIGRIGTAGGTGYALEYGGEAIRSLSMEGRMTICNMSIEAGARTGMVAVDDTT
IEYVRGRPNAPEGALWDQAVASWRHLVSDEDAAFDRVVELHADEIEPQVTWGTSPEMVASVNRRVPDPAEESDAVRARAM
GRALEYMGLEPGTPLTDIPMDKIFIGSCTNARIEDLREAAAVVHGRRVAENIRQALVVPGSGVVKQQAEGEGLDRVFLDA
GFEWREPGCSMCLGMNPDRLEPGERCASTSNRNFEGRQGQGGRTHLASPAMVAAAAIHGHFVDIRE

Sequences:

>Translated_466_residues
MTGKTLYDKLWDSHVVTEYDDGSALLYIDRQLLHEVTSPQAFEGLRLAGRQPWRVASNLAVTDHNVPTTDRSQPVEDPVS
RVQIETLDRNCKDFQVIEFGIRDPRQGIVHVVGPEQGTTLPGMTLVCGDSHTSTHGALGALAFGVGTSEVEHVLATQTLV
QKKARTMLIRIDGQLGRGVTAKDIILAIIGRIGTAGGTGYALEYGGEAIRSLSMEGRMTICNMSIEAGARTGMVAVDDTT
IEYVRGRPNAPEGALWDQAVASWRHLVSDEDAAFDRVVELHADEIEPQVTWGTSPEMVASVNRRVPDPAEESDAVRARAM
GRALEYMGLEPGTPLTDIPMDKIFIGSCTNARIEDLREAAAVVHGRRVAENIRQALVVPGSGVVKQQAEGEGLDRVFLDA
GFEWREPGCSMCLGMNPDRLEPGERCASTSNRNFEGRQGQGGRTHLASPAMVAAAAIHGHFVDIRE
>Mature_465_residues
TGKTLYDKLWDSHVVTEYDDGSALLYIDRQLLHEVTSPQAFEGLRLAGRQPWRVASNLAVTDHNVPTTDRSQPVEDPVSR
VQIETLDRNCKDFQVIEFGIRDPRQGIVHVVGPEQGTTLPGMTLVCGDSHTSTHGALGALAFGVGTSEVEHVLATQTLVQ
KKARTMLIRIDGQLGRGVTAKDIILAIIGRIGTAGGTGYALEYGGEAIRSLSMEGRMTICNMSIEAGARTGMVAVDDTTI
EYVRGRPNAPEGALWDQAVASWRHLVSDEDAAFDRVVELHADEIEPQVTWGTSPEMVASVNRRVPDPAEESDAVRARAMG
RALEYMGLEPGTPLTDIPMDKIFIGSCTNARIEDLREAAAVVHGRRVAENIRQALVVPGSGVVKQQAEGEGLDRVFLDAG
FEWREPGCSMCLGMNPDRLEPGERCASTSNRNFEGRQGQGGRTHLASPAMVAAAAIHGHFVDIRE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=370, Percent_Identity=30, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI8659555, Length=449, Percent_Identity=27.8396436525612, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI41352693, Length=442, Percent_Identity=26.6968325791855, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=63.6363636363636, Blast_Score=603, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=445, Percent_Identity=26.9662921348315, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25149337, Length=380, Percent_Identity=29.4736842105263, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=372, Percent_Identity=29.3010752688172, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=311, Percent_Identity=28.6173633440514, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=468, Percent_Identity=27.3504273504274, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=61.6204690831556, Blast_Score=595, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6323335, Length=391, Percent_Identity=29.6675191815857, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=375, Percent_Identity=28.2666666666667, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=354, Percent_Identity=27.1186440677966, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28571643, Length=480, Percent_Identity=27.0833333333333, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281365315, Length=377, Percent_Identity=27.5862068965517, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17864292, Length=377, Percent_Identity=27.5862068965517, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161076999, Length=375, Percent_Identity=27.2, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=27.4891774891775, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137564, Length=444, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_HALHL (A1WY14)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003378.1
- STRING:   A1WY14
- GeneID:   4711017
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1812
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1WY14
- ProtClustDB:   PRK05478
- BioCyc:   HHAL349124:HHAL_1812-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50495; Mature: 50363

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKTLYDKLWDSHVVTEYDDGSALLYIDRQLLHEVTSPQAFEGLRLAGRQPWRVASNLA
CCCCHHHHHHCCCCCEEEECCCCEEEEECHHHHHHHCCCHHHHHHHHCCCCCCHHHCCEE
VTDHNVPTTDRSQPVEDPVSRVQIETLDRNCKDFQVIEFGIRDPRQGIVHVVGPEQGTTL
EECCCCCCCCCCCCCHHHHHHEEHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCC
PGMTLVCGDSHTSTHGALGALAFGVGTSEVEHVLATQTLVQKKARTMLIRIDGQLGRGVT
CCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
AKDIILAIIGRIGTAGGTGYALEYGGEAIRSLSMEGRMTICNMSIEAGARTGMVAVDDTT
HHHHHHHHHHHHCCCCCCCEEEHHCHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCH
IEYVRGRPNAPEGALWDQAVASWRHLVSDEDAAFDRVVELHADEIEPQVTWGTSPEMVAS
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
VNRRVPDPAEESDAVRARAMGRALEYMGLEPGTPLTDIPMDKIFIGSCTNARIEDLREAA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
AVVHGRRVAENIRQALVVPGSGVVKQQAEGEGLDRVFLDAGFEWREPGCSMCLGMNPDRL
HHHHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCC
EPGERCASTSNRNFEGRQGQGGRTHLASPAMVAAAAIHGHFVDIRE
CCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECC
>Mature Secondary Structure 
TGKTLYDKLWDSHVVTEYDDGSALLYIDRQLLHEVTSPQAFEGLRLAGRQPWRVASNLA
CCCHHHHHHCCCCCEEEECCCCEEEEECHHHHHHHCCCHHHHHHHHCCCCCCHHHCCEE
VTDHNVPTTDRSQPVEDPVSRVQIETLDRNCKDFQVIEFGIRDPRQGIVHVVGPEQGTTL
EECCCCCCCCCCCCCHHHHHHEEHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCC
PGMTLVCGDSHTSTHGALGALAFGVGTSEVEHVLATQTLVQKKARTMLIRIDGQLGRGVT
CCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
AKDIILAIIGRIGTAGGTGYALEYGGEAIRSLSMEGRMTICNMSIEAGARTGMVAVDDTT
HHHHHHHHHHHHCCCCCCCEEEHHCHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCH
IEYVRGRPNAPEGALWDQAVASWRHLVSDEDAAFDRVVELHADEIEPQVTWGTSPEMVAS
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
VNRRVPDPAEESDAVRARAMGRALEYMGLEPGTPLTDIPMDKIFIGSCTNARIEDLREAA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
AVVHGRRVAENIRQALVVPGSGVVKQQAEGEGLDRVFLDAGFEWREPGCSMCLGMNPDRL
HHHHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCC
EPGERCASTSNRNFEGRQGQGGRTHLASPAMVAAAAIHGHFVDIRE
CCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA