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The map label for this gene is leuB [H]

Identifier: 121998589

GI number: 121998589

Start: 1983109

End: 1984179

Strand: Reverse

Name: leuB [H]

Synonym: Hhal_1810

Alternate gene names: 121998589

Gene position: 1984179-1983109 (Counterclockwise)

Preceding gene: 121998590

Following gene: 121998587

Centisome position: 74.08

GC content: 69.84

Gene sequence:

>1071_bases
ATGGCCCAGAAGATCCTGCTGCTCCCCGGGGACGGTATCGGCCCGGAGATCACCGCGGAGGCCCGGCGTGTGCTCGAGGC
GCTGAATAAGCGCTACGGGGTCGGCTGCGAGATGGAGACGGCGCCCATTGGCGGCGCCGGTTACGACGCCGCCGGCCAGC
CCCTGCCCGACGAGACCCTGCGCCTGGCGCGGGAGGCGGATGCGGTGCTGCTGGGGGCCGTCGGCGGGCCGCAGTACGAT
GCGCTCCCACGGGACGTCCGACCGGAGCGGGGGCTTCTGGCGATCCGCTCGGAGCTGGGTCTGTTCGGCAATCTGCGCCC
GGCCATCCTGTATCCGCAGCTGGCCGGCGCCTCGGCGCTGCGGGAAGATGTTGTCGCGGGGCTGGATATCCTCATCGTTC
GCGAACTCACCGGCGGGATCTACTTTGGCCAGCCCCGCGGGATCCGCACCCTGGACAGTGGCGAGCGTCAGGGCTTCAAC
ACCGAGGTCTATAGCGAGTCGGAGATCGAGCGCATTGCCCGTCTCGCCTTCGCCGCCGCCGAGCAGCGCCAGGGACGGGT
CTGCTCGGTGGACAAGGCCAATGTCCTGGAAAGCTCGGAGCTATGGCGCGAAGTGGTCGAGCGGGTGGCGGCGGACTACC
CGGGTGTCGAGCTCAGCCACATGTACGTGGACAACGCCGCCATGCAGCTGGTGCGTGCGCCGAAGCAGTTCGATGTGGTG
GTCACCGGGAACCTGTTCGGGGACATCCTCTCGGATTGCGCCGCGCAGCTGACCGGCTCCATCGGCATGCTCCCGTCCGC
CTCCCTCGATGAACACGGCAAGGGGCTCTACGAGCCGGTCCACGGCTCGGCGCCGGATATTGCCGGGCAGGACAAGGCGA
ACCCGCTAGCCACCATCCTCTCGGTGGCCATGATGCTGCGCTACAGCCTGGGCGCGGGTGAGGCCGCGGACCGGGTCGAG
GCCGCCGTGGGGGCGGTGCTCGAGGAGGGGTTGCGCACTCCGGACCTGCAGGGCGGCAACCGGCCGGTGGGCACTCGTGA
GATGGGTGAGGCAGTGGCGGGGCGGCTGTGA

Upstream 100 bases:

>100_bases
CCATCCAGGCCTATGAGCAGCGCCGCCTGCAGGAAGCCCCCTGGCTGTTCCTTGAGCGCTGAACCCGGAAACCTGAAATC
TGAATGGTGAGTGCGTAACG

Downstream 100 bases:

>100_bases
CTCGCGACGTCCAGCATTCCGACGCCCGCCGTTCCGGGCGAAAACACGAGCCGGCCGCCCCTAAAGGGCGGCCGGCTCGT
GCATGACGGTCGTTATGTGT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAQKILLLPGDGIGPEITAEARRVLEALNKRYGVGCEMETAPIGGAGYDAAGQPLPDETLRLAREADAVLLGAVGGPQYD
ALPRDVRPERGLLAIRSELGLFGNLRPAILYPQLAGASALREDVVAGLDILIVRELTGGIYFGQPRGIRTLDSGERQGFN
TEVYSESEIERIARLAFAAAEQRQGRVCSVDKANVLESSELWREVVERVAADYPGVELSHMYVDNAAMQLVRAPKQFDVV
VTGNLFGDILSDCAAQLTGSIGMLPSASLDEHGKGLYEPVHGSAPDIAGQDKANPLATILSVAMMLRYSLGAGEAADRVE
AAVGAVLEEGLRTPDLQGGNRPVGTREMGEAVAGRL

Sequences:

>Translated_356_residues
MAQKILLLPGDGIGPEITAEARRVLEALNKRYGVGCEMETAPIGGAGYDAAGQPLPDETLRLAREADAVLLGAVGGPQYD
ALPRDVRPERGLLAIRSELGLFGNLRPAILYPQLAGASALREDVVAGLDILIVRELTGGIYFGQPRGIRTLDSGERQGFN
TEVYSESEIERIARLAFAAAEQRQGRVCSVDKANVLESSELWREVVERVAADYPGVELSHMYVDNAAMQLVRAPKQFDVV
VTGNLFGDILSDCAAQLTGSIGMLPSASLDEHGKGLYEPVHGSAPDIAGQDKANPLATILSVAMMLRYSLGAGEAADRVE
AAVGAVLEEGLRTPDLQGGNRPVGTREMGEAVAGRL
>Mature_355_residues
AQKILLLPGDGIGPEITAEARRVLEALNKRYGVGCEMETAPIGGAGYDAAGQPLPDETLRLAREADAVLLGAVGGPQYDA
LPRDVRPERGLLAIRSELGLFGNLRPAILYPQLAGASALREDVVAGLDILIVRELTGGIYFGQPRGIRTLDSGERQGFNT
EVYSESEIERIARLAFAAAEQRQGRVCSVDKANVLESSELWREVVERVAADYPGVELSHMYVDNAAMQLVRAPKQFDVVV
TGNLFGDILSDCAAQLTGSIGMLPSASLDEHGKGLYEPVHGSAPDIAGQDKANPLATILSVAMMLRYSLGAGEAADRVEA
AVGAVLEEGLRTPDLQGGNRPVGTREMGEAVAGRL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=34.1176470588235, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=29.0322580645161, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=311, Percent_Identity=28.9389067524116, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178821, Length=311, Percent_Identity=28.9389067524116, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI28178838, Length=338, Percent_Identity=28.698224852071, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI28178819, Length=197, Percent_Identity=30.9644670050761, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=56.25, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1788101, Length=347, Percent_Identity=37.463976945245, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787381, Length=393, Percent_Identity=28.498727735369, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=35.8600583090379, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17505779, Length=343, Percent_Identity=29.4460641399417, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=28.4090909090909, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=347, Percent_Identity=29.971181556196, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=46.9613259668508, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=36.4640883977901, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=363, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=345, Percent_Identity=31.0144927536232, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=34.6938775510204, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=34.4311377245509, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24661184, Length=345, Percent_Identity=31.8840579710145, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=30.7246376811594, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=30.7246376811594, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=30.7246376811594, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=30.7246376811594, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI161078639, Length=343, Percent_Identity=30.9037900874636, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=27.3529411764706, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=27.3529411764706, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130355, Length=220, Percent_Identity=26.8181818181818, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37741; Mature: 37610

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKILLLPGDGIGPEITAEARRVLEALNKRYGVGCEMETAPIGGAGYDAAGQPLPDETL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHH
RLAREADAVLLGAVGGPQYDALPRDVRPERGLLAIRSELGLFGNLRPAILYPQLAGASAL
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHEECCCCCCHHHH
REDVVAGLDILIVRELTGGIYFGQPRGIRTLDSGERQGFNTEVYSESEIERIARLAFAAA
HHHHHHCHHHEEEEHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQRQGRVCSVDKANVLESSELWREVVERVAADYPGVELSHMYVDNAAMQLVRAPKQFDVV
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCEEEE
VTGNLFGDILSDCAAQLTGSIGMLPSASLDEHGKGLYEPVHGSAPDIAGQDKANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SVAMMLRYSLGAGEAADRVEAAVGAVLEEGLRTPDLQGGNRPVGTREMGEAVAGRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AQKILLLPGDGIGPEITAEARRVLEALNKRYGVGCEMETAPIGGAGYDAAGQPLPDETL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHH
RLAREADAVLLGAVGGPQYDALPRDVRPERGLLAIRSELGLFGNLRPAILYPQLAGASAL
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHEECCCCCCHHHH
REDVVAGLDILIVRELTGGIYFGQPRGIRTLDSGERQGFNTEVYSESEIERIARLAFAAA
HHHHHHCHHHEEEEHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQRQGRVCSVDKANVLESSELWREVVERVAADYPGVELSHMYVDNAAMQLVRAPKQFDVV
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCEEEE
VTGNLFGDILSDCAAQLTGSIGMLPSASLDEHGKGLYEPVHGSAPDIAGQDKANPLATIL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SVAMMLRYSLGAGEAADRVEAAVGAVLEEGLRTPDLQGGNRPVGTREMGEAVAGRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9108278; 10984043 [H]