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The map label for this gene is metZ [H]

Identifier: 121998576

GI number: 121998576

Start: 1968924

End: 1970117

Strand: Reverse

Name: metZ [H]

Synonym: Hhal_1797

Alternate gene names: 121998576

Gene position: 1970117-1968924 (Counterclockwise)

Preceding gene: 121998577

Following gene: 121998574

Centisome position: 73.55

GC content: 69.26

Gene sequence:

>1194_bases
ATGTGCACAACCAGGCCTGATGACGATCCGCTCGCCGGGTTCGAATCCCGGGCGGTGCGAGCCGGTCAGGTGCGCACCGA
TGCCCAGGAGCAGTCGGAGCCCATCTACCCGACCTCCAGCTTTACCTTCGAGAGTGCCGCACAGGCGGCCGCGCGTTTCT
CCGGTGAGGACCCCGGGAACGTCTACTCGCGTTTCACCAACCCGACGGTGCGGACCTTCTGCGACCGCCTGGCCGCCCTG
GAGGGCGGGCAGGCCTGCGTCGGGACGGCTTCAGGCATGTCGGCGGTGCTGGCGACGTGCCTGGGGCTGCTGCAGGCCGG
CGACCACGTGGTCGCCTCGCGGACCCTCTTCGGGACCACCCTGTCGCTGCTGACCAAGTACCTGCCGCGCTGGGGCATCG
AGGTCAGCTGGGTGCCGTTGAGCGACGAGCGGGCCTGGGCTGATGCGGTGCAGCCGAACACGCGCCTGCTCTTTGCTGAG
ACGCCCTCCAACCCCCTCAACGAGGTGGTGGACATCCGCCGCCTCGCGGAGGTGGCCCATGCCCACGAAGCCCTGTTGGC
GATCGACAACTGCTTCTGTACCCCCGCCCTGCAGCGCCCGCTGGAGATGGGGGCCGACCTGGTGATCCACTCGGCCACCA
AGTACCTGGACGGTCAAGGTCGCTGTGTCGGTGGCGCGGTGGTTGGCGACGCCCAGCGCGTGGGGGAAGAGATCCACGGT
TTCATCCGCACCGCCGGGCCGTGCATGAGCCCGTTCAACGCCTGGGTGTTCCTCAAGGGATTGGAGACGCTGTCCCTGCG
CATGCATGCGCACAGCCGGAATGCCCAGCAGGTGGCGGAGTGGCTGCAGGGCCATCCCGGCGTCGAGCGGGTCCACTACG
CCGGGCTGCCGGACCATCCCCACCACCGCCTGGCCGCGGCGCAGCAGAGCGGGTTCGGCGGGATTGTGGCCTTCGAGCTC
CCCGGAGGCCGGGAGGCAGCCTGGCGTCTGATCGACAGCACGCGCATGCTGTCGATCACCGGCAACCTGGGGGACACCAA
GTCCACCATCACCCATCCGGCGACCACCACCCACGGCACCATCTCCGATGAGTTGCGCGCGGCCGCCGGCATCCGCGAGG
GGCTGGTGCGGGTTTCCGTTGGGTTGGAGGATCCGGCGGATATCATCCGCGACCTGGAGCGCGGCCTGGGGTGA

Upstream 100 bases:

>100_bases
ACCGGAGGCGTCAGCCAGGACTATCTGCGTGGCCTCTCTCAGGCACGTGCCGATGCCGCCAGGGAACAGGCGGGCAGCGA
CCAAGACTTGGAGATCGTGG

Downstream 100 bases:

>100_bases
GGCCGCGCCGGGCAGCGCGATCAGCCGGTGGGTAGCTCAGCGGCGTGGGCGCGGATGGTGTTCAGCAGGCCACTGTGGAC
CTTGGGGGCGCCCGCGACGA

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MCTTRPDDDPLAGFESRAVRAGQVRTDAQEQSEPIYPTSSFTFESAAQAAARFSGEDPGNVYSRFTNPTVRTFCDRLAAL
EGGQACVGTASGMSAVLATCLGLLQAGDHVVASRTLFGTTLSLLTKYLPRWGIEVSWVPLSDERAWADAVQPNTRLLFAE
TPSNPLNEVVDIRRLAEVAHAHEALLAIDNCFCTPALQRPLEMGADLVIHSATKYLDGQGRCVGGAVVGDAQRVGEEIHG
FIRTAGPCMSPFNAWVFLKGLETLSLRMHAHSRNAQQVAEWLQGHPGVERVHYAGLPDHPHHRLAAAQQSGFGGIVAFEL
PGGREAAWRLIDSTRMLSITGNLGDTKSTITHPATTTHGTISDELRAAAGIREGLVRVSVGLEDPADIIRDLERGLG

Sequences:

>Translated_397_residues
MCTTRPDDDPLAGFESRAVRAGQVRTDAQEQSEPIYPTSSFTFESAAQAAARFSGEDPGNVYSRFTNPTVRTFCDRLAAL
EGGQACVGTASGMSAVLATCLGLLQAGDHVVASRTLFGTTLSLLTKYLPRWGIEVSWVPLSDERAWADAVQPNTRLLFAE
TPSNPLNEVVDIRRLAEVAHAHEALLAIDNCFCTPALQRPLEMGADLVIHSATKYLDGQGRCVGGAVVGDAQRVGEEIHG
FIRTAGPCMSPFNAWVFLKGLETLSLRMHAHSRNAQQVAEWLQGHPGVERVHYAGLPDHPHHRLAAAQQSGFGGIVAFEL
PGGREAAWRLIDSTRMLSITGNLGDTKSTITHPATTTHGTISDELRAAAGIREGLVRVSVGLEDPADIIRDLERGLG
>Mature_397_residues
MCTTRPDDDPLAGFESRAVRAGQVRTDAQEQSEPIYPTSSFTFESAAQAAARFSGEDPGNVYSRFTNPTVRTFCDRLAAL
EGGQACVGTASGMSAVLATCLGLLQAGDHVVASRTLFGTTLSLLTKYLPRWGIEVSWVPLSDERAWADAVQPNTRLLFAE
TPSNPLNEVVDIRRLAEVAHAHEALLAIDNCFCTPALQRPLEMGADLVIHSATKYLDGQGRCVGGAVVGDAQRVGEEIHG
FIRTAGPCMSPFNAWVFLKGLETLSLRMHAHSRNAQQVAEWLQGHPGVERVHYAGLPDHPHHRLAAAQQSGFGGIVAFEL
PGGREAAWRLIDSTRMLSITGNLGDTKSTITHPATTTHGTISDELRAAAGIREGLVRVSVGLEDPADIIRDLERGLG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=371, Percent_Identity=33.4231805929919, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI299473759, Length=371, Percent_Identity=30.4582210242588, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI25453487, Length=370, Percent_Identity=28.6486486486486, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1790375, Length=363, Percent_Identity=37.7410468319559, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1789383, Length=378, Percent_Identity=29.1005291005291, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=375, Percent_Identity=34.1333333333333, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=375, Percent_Identity=33.6, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=35.0404312668464, Blast_Score=224, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=34.1836734693878, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=32.4825986078886, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=26.2899262899263, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=312, Percent_Identity=25.9615384615385, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=348, Percent_Identity=25.2873563218391, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=34.5360824742268, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42635; Mature: 42635

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTRPDDDPLAGFESRAVRAGQVRTDAQEQSEPIYPTSSFTFESAAQAAARFSGEDPGN
CCCCCCCCCCCCCCHHHCEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHH
VYSRFTNPTVRTFCDRLAALEGGQACVGTASGMSAVLATCLGLLQAGDHVVASRTLFGTT
HHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSLLTKYLPRWGIEVSWVPLSDERAWADAVQPNTRLLFAETPSNPLNEVVDIRRLAEVAH
HHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AHEALLAIDNCFCTPALQRPLEMGADLVIHSATKYLDGQGRCVGGAVVGDAQRVGEEIHG
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHCCCCCCEECCCEECCHHHHHHHHHH
FIRTAGPCMSPFNAWVFLKGLETLSLRMHAHSRNAQQVAEWLQGHPGVERVHYAGLPDHP
HHHHCCCCCCCHHHEEEEECHHHHEEEEHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCC
HHRLAAAQQSGFGGIVAFELPGGREAAWRLIDSTRMLSITGNLGDTKSTITHPATTTHGT
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC
ISDELRAAAGIREGLVRVSVGLEDPADIIRDLERGLG
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MCTTRPDDDPLAGFESRAVRAGQVRTDAQEQSEPIYPTSSFTFESAAQAAARFSGEDPGN
CCCCCCCCCCCCCCHHHCEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHH
VYSRFTNPTVRTFCDRLAALEGGQACVGTASGMSAVLATCLGLLQAGDHVVASRTLFGTT
HHHHCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSLLTKYLPRWGIEVSWVPLSDERAWADAVQPNTRLLFAETPSNPLNEVVDIRRLAEVAH
HHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AHEALLAIDNCFCTPALQRPLEMGADLVIHSATKYLDGQGRCVGGAVVGDAQRVGEEIHG
HHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHCCCCCCEECCCEECCHHHHHHHHHH
FIRTAGPCMSPFNAWVFLKGLETLSLRMHAHSRNAQQVAEWLQGHPGVERVHYAGLPDHP
HHHHCCCCCCCHHHEEEEECHHHHEEEEHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCC
HHRLAAAQQSGFGGIVAFELPGGREAAWRLIDSTRMLSITGNLGDTKSTITHPATTTHGT
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC
ISDELRAAAGIREGLVRVSVGLEDPADIIRDLERGLG
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]