Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 121998577

GI number: 121998577

Start: 1970098

End: 1971612

Strand: Reverse

Name: purF [H]

Synonym: Hhal_1798

Alternate gene names: 121998577

Gene position: 1971612-1970098 (Counterclockwise)

Preceding gene: 121998578

Following gene: 121998576

Centisome position: 73.61

GC content: 65.02

Gene sequence:

>1515_bases
ATGTGTGGCGTAATCGGAATGGTGGCCAAGGGGCCGGTGAACCAGGACCTTTACGAGGGGCTGACCGTGCTCCAGCACCG
GGGTCAGGACGCTGCCGGGATCATTACCTATGACCAGGGGCAGTTGAGCCTGCGCAAGAGCAACGGCCTGGTGCGGGATG
TCTTCCGGACCCGGCACATGATGCGCCTGACCGGCAATGTCGGTATTGGCCACGTTCGTTATCCCACCGCCGGCTGCGAG
AGTTCCGCCGAAGCGCAGCCTTTCTACGTCAACTCGCCCTACGGCATCTCGCTGGCGCACAACGGCAACCTGACCAATAC
CGAGGAGCTCAAGCAGGAGCTCTTCCGTACGGACCGACGCCATATCAACACCAACTCCGACTCGGAGATCCTGCTCAACA
TCCTCGCCCACGAGTTGAGTGTCTGCCCCCACGGCCGGCTCGAGCCCAGCGATCTGTTCAGTGCCGTCGAGCAGGTCCAC
CGGCGCTGCCACGGCGCCTACTCGGCCGTGGCGATGATCAACGGCTACGGCATTCTCGCCTTCCGTGACCCCTTCGGCAT
CCGCCCGCTGTGCTTCGGCGAGCGCGATACCGAGCAGGGGCGGGAGTACCTGATCGCTTCGGAGAGCGTGGCGCTGGATA
TGCTCGGCTTCCGCCTGGTGCGCGAGGTGGCTCCGGGCGAGGCGATCTTCATCGACATGGATGGTGTCCTGCACACCCGC
CAGTGTGCCGAGGATCCTATCCACGCCCCGTGCCTGTTCGAGTACGTCTACCTCTCCCGGCCCGACTCCATTGTCGACGG
CGTGGCCGTGCACAAGGCACGTCTGCGTATGGGCGAGCGGCTGGCGGCCAAGATCCGGCGCGAGTGGCCCGATCACGATA
TCGATGTGATCATCCCGATCCCGGACACCAGCCGCACCGTCGCCATGCAGCTGGCCTACCAGTTGGGCGTGGAGTCCCGG
GAGGGCTTCATCAAGAACCGCTACGTCGGGCGCACCTTCATCATGCCCGGGCAGGCGGCGCGCAAGAAATCGGTGCGTCA
GAAGCTCAACCCCATCCCGCTGGAGTTCGCGGGCAAGAACGTCCTGCTTGTGGACGACTCGGTGGTCCGGGGCACCACCT
CGCAGCAGCTGGTGCAGCTGGCCCGCGAGTCCGGGGCGCGGCGGGTCTATCTCGCCTCCGCCGCCCCGCCGGTGCGCTAC
CCCAACGTCTACGGTATCGATATGCCGGCCCCCGAAGAGCTCATCGCCCATAACCGAGATGAGGCCGAGATCACGGCGGC
GATCGGTGCCGACCGCATGATCTACCAGGAACTCGACGACCTGGAAGCGGCCGTGGCCGATGGCAATCCGGAGATCCGGC
AGTTCGATAGTTCCTGCTTCAACGGGCACTACGTGACCGGAGGCGTCAGCCAGGACTATCTGCGTGGCCTCTCTCAGGCA
CGTGCCGATGCCGCCAGGGAACAGGCGGGCAGCGACCAAGACTTGGAGATCGTGGATGTGCACAACCAGGCCTGA

Upstream 100 bases:

>100_bases
GCTTCGATGGCTTGCCGGATTACTGGGCCGAATACTGCGCCGGGCGGCGGCCGTGAGTCCGTCGGCCGTTCCGGTGCCTA
GCAAGAGGTAGGAGCAATCC

Downstream 100 bases:

>100_bases
TGACGATCCGCTCGCCGGGTTCGAATCCCGGGCGGTGCGAGCCGGTCAGGTGCGCACCGATGCCCAGGAGCAGTCGGAGC
CCATCTACCCGACCTCCAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGVIGMVAKGPVNQDLYEGLTVLQHRGQDAAGIITYDQGQLSLRKSNGLVRDVFRTRHMMRLTGNVGIGHVRYPTAGCE
SSAEAQPFYVNSPYGISLAHNGNLTNTEELKQELFRTDRRHINTNSDSEILLNILAHELSVCPHGRLEPSDLFSAVEQVH
RRCHGAYSAVAMINGYGILAFRDPFGIRPLCFGERDTEQGREYLIASESVALDMLGFRLVREVAPGEAIFIDMDGVLHTR
QCAEDPIHAPCLFEYVYLSRPDSIVDGVAVHKARLRMGERLAAKIRREWPDHDIDVIIPIPDTSRTVAMQLAYQLGVESR
EGFIKNRYVGRTFIMPGQAARKKSVRQKLNPIPLEFAGKNVLLVDDSVVRGTTSQQLVQLARESGARRVYLASAAPPVRY
PNVYGIDMPAPEELIAHNRDEAEITAAIGADRMIYQELDDLEAAVADGNPEIRQFDSSCFNGHYVTGGVSQDYLRGLSQA
RADAAREQAGSDQDLEIVDVHNQA

Sequences:

>Translated_504_residues
MCGVIGMVAKGPVNQDLYEGLTVLQHRGQDAAGIITYDQGQLSLRKSNGLVRDVFRTRHMMRLTGNVGIGHVRYPTAGCE
SSAEAQPFYVNSPYGISLAHNGNLTNTEELKQELFRTDRRHINTNSDSEILLNILAHELSVCPHGRLEPSDLFSAVEQVH
RRCHGAYSAVAMINGYGILAFRDPFGIRPLCFGERDTEQGREYLIASESVALDMLGFRLVREVAPGEAIFIDMDGVLHTR
QCAEDPIHAPCLFEYVYLSRPDSIVDGVAVHKARLRMGERLAAKIRREWPDHDIDVIIPIPDTSRTVAMQLAYQLGVESR
EGFIKNRYVGRTFIMPGQAARKKSVRQKLNPIPLEFAGKNVLLVDDSVVRGTTSQQLVQLARESGARRVYLASAAPPVRY
PNVYGIDMPAPEELIAHNRDEAEITAAIGADRMIYQELDDLEAAVADGNPEIRQFDSSCFNGHYVTGGVSQDYLRGLSQA
RADAAREQAGSDQDLEIVDVHNQA
>Mature_504_residues
MCGVIGMVAKGPVNQDLYEGLTVLQHRGQDAAGIITYDQGQLSLRKSNGLVRDVFRTRHMMRLTGNVGIGHVRYPTAGCE
SSAEAQPFYVNSPYGISLAHNGNLTNTEELKQELFRTDRRHINTNSDSEILLNILAHELSVCPHGRLEPSDLFSAVEQVH
RRCHGAYSAVAMINGYGILAFRDPFGIRPLCFGERDTEQGREYLIASESVALDMLGFRLVREVAPGEAIFIDMDGVLHTR
QCAEDPIHAPCLFEYVYLSRPDSIVDGVAVHKARLRMGERLAAKIRREWPDHDIDVIIPIPDTSRTVAMQLAYQLGVESR
EGFIKNRYVGRTFIMPGQAARKKSVRQKLNPIPLEFAGKNVLLVDDSVVRGTTSQQLVQLARESGARRVYLASAAPPVRY
PNVYGIDMPAPEELIAHNRDEAEITAAIGADRMIYQELDDLEAAVADGNPEIRQFDSSCFNGHYVTGGVSQDYLRGLSQA
RADAAREQAGSDQDLEIVDVHNQA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=471, Percent_Identity=36.3057324840764, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=60.3960396039604, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=30.0578034682081, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=32.9192546583851, Blast_Score=251, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=506, Percent_Identity=48.8142292490119, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=34.9494949494949, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI28573187, Length=498, Percent_Identity=34.7389558232932, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24659604, Length=476, Percent_Identity=35.0840336134454, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55823; Mature: 55823

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVIGMVAKGPVNQDLYEGLTVLQHRGQDAAGIITYDQGQLSLRKSNGLVRDVFRTRHM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
MRLTGNVGIGHVRYPTAGCESSAEAQPFYVNSPYGISLAHNGNLTNTEELKQELFRTDRR
HEEECCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTNSDSEILLNILAHELSVCPHGRLEPSDLFSAVEQVHRRCHGAYSAVAMINGYGILA
HCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
FRDPFGIRPLCFGERDTEQGREYLIASESVALDMLGFRLVREVAPGEAIFIDMDGVLHTR
ECCCCCCCCEECCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
QCAEDPIHAPCLFEYVYLSRPDSIVDGVAVHKARLRMGERLAAKIRREWPDHDIDVIIPI
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTVAMQLAYQLGVESREGFIKNRYVGRTFIMPGQAARKKSVRQKLNPIPLEFAGKN
CCCCHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCE
VLLVDDSVVRGTTSQQLVQLARESGARRVYLASAAPPVRYPNVYGIDMPAPEELIAHNRD
EEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCC
EAEITAAIGADRMIYQELDDLEAAVADGNPEIRQFDSSCFNGHYVTGGVSQDYLRGLSQA
CCEEEEHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RADAAREQAGSDQDLEIVDVHNQA
HHHHHHHHCCCCCCEEEEECCCCC
>Mature Secondary Structure
MCGVIGMVAKGPVNQDLYEGLTVLQHRGQDAAGIITYDQGQLSLRKSNGLVRDVFRTRHM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHH
MRLTGNVGIGHVRYPTAGCESSAEAQPFYVNSPYGISLAHNGNLTNTEELKQELFRTDRR
HEEECCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HINTNSDSEILLNILAHELSVCPHGRLEPSDLFSAVEQVHRRCHGAYSAVAMINGYGILA
HCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
FRDPFGIRPLCFGERDTEQGREYLIASESVALDMLGFRLVREVAPGEAIFIDMDGVLHTR
ECCCCCCCCEECCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
QCAEDPIHAPCLFEYVYLSRPDSIVDGVAVHKARLRMGERLAAKIRREWPDHDIDVIIPI
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTVAMQLAYQLGVESREGFIKNRYVGRTFIMPGQAARKKSVRQKLNPIPLEFAGKN
CCCCHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCE
VLLVDDSVVRGTTSQQLVQLARESGARRVYLASAAPPVRYPNVYGIDMPAPEELIAHNRD
EEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCC
EAEITAAIGADRMIYQELDDLEAAVADGNPEIRQFDSSCFNGHYVTGGVSQDYLRGLSQA
CCEEEEHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RADAAREQAGSDQDLEIVDVHNQA
HHHHHHHHCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]