Click here to switch to the map view.

The map label for this gene is greA

Identifier: 121998553

GI number: 121998553

Start: 1946956

End: 1947432

Strand: Reverse

Name: greA

Synonym: Hhal_1774

Alternate gene names: 121998553

Gene position: 1947432-1946956 (Counterclockwise)

Preceding gene: 121998554

Following gene: 121998552

Centisome position: 72.71

GC content: 61.01

Gene sequence:

>477_bases
ATGAGCAAGATACCGTTGACGATTCGTGGTGCAGAGAAGCTGCGTGAAGAGCTTCACCGGCTCAAGCACGAAGACCGGCC
GCGGGTTATTCAGGCCATTGCCGAGGCGCGTGAGCACGGCGATCTGAAGGAGAACGCCGAGTACCACGCCGCGCGCGAGC
AACAGAGCTTTATCGAGGGCCGAATCCAGGAGATCGAGGGCAAGCTGTCCAATGCCCAGATCATCGATCCGGCTGCGGTC
CAGGCGGCGCCGAAGATTGTCTTCGGCGCCACTGTGGCGCTTGAAGACACGGACACTGAAGAGACGGTCACCTATCAGAT
CGTTGGCGAGGACGAGGCGGACATCAAGCAACACCTGATCTCGGTCAATTCGCCCATCGCCCGCGCCCTGATCGGCAAGG
AGGAGGGTGACGAGGCTGTGGTTCAGGCCCCGGGCGGAGAGCGGACCTACGAGATCGTCGAGGTTCGGTATGAATAG

Upstream 100 bases:

>100_bases
CGACGACCATCGCGGGCGCTCGGGCGACGTGCCTGGCGCTGGATCACATGAAGGACTGGGAGGCCCGCCCCCTCGATGCC
CTGCACAGGGAGATGACGGG

Downstream 100 bases:

>100_bases
TCCCGGACGGTGCGGGCTGGCTGTGGAGGTGCGGTGCTGATCCGCGCCGCAGAGCGGGTAATGTTGACGGTGCTGGCCGG
CGCGCTGTGGAGCGGTGGCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSKIPLTIRGAEKLREELHRLKHEDRPRVIQAIAEAREHGDLKENAEYHAAREQQSFIEGRIQEIEGKLSNAQIIDPAAV
QAAPKIVFGATVALEDTDTEETVTYQIVGEDEADIKQHLISVNSPIARALIGKEEGDEAVVQAPGGERTYEIVEVRYE

Sequences:

>Translated_158_residues
MSKIPLTIRGAEKLREELHRLKHEDRPRVIQAIAEAREHGDLKENAEYHAAREQQSFIEGRIQEIEGKLSNAQIIDPAAV
QAAPKIVFGATVALEDTDTEETVTYQIVGEDEADIKQHLISVNSPIARALIGKEEGDEAVVQAPGGERTYEIVEVRYE
>Mature_157_residues
SKIPLTIRGAEKLREELHRLKHEDRPRVIQAIAEAREHGDLKENAEYHAAREQQSFIEGRIQEIEGKLSNAQIIDPAAVQ
AAPKIVFGATVALEDTDTEETVTYQIVGEDEADIKQHLISVNSPIARALIGKEEGDEAVVQAPGGERTYEIVEVRYE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=64.3312101910828, Blast_Score=207, Evalue=3e-55,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=39.1025641025641, Blast_Score=103, Evalue=6e-24,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_HALHL (A1WXX6)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003340.1
- ProteinModelPortal:   A1WXX6
- SMR:   A1WXX6
- STRING:   A1WXX6
- GeneID:   4711011
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1774
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   A1WXX6
- ProtClustDB:   PRK00226
- BioCyc:   HHAL349124:HHAL_1774-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17607; Mature: 17476

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPLTIRGAEKLREELHRLKHEDRPRVIQAIAEAREHGDLKENAEYHAAREQQSFIEG
CCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIQEIEGKLSNAQIIDPAAVQAAPKIVFGATVALEDTDTEETVTYQIVGEDEADIKQHLI
HHHHHHCCCCCCEEECHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHH
SVNSPIARALIGKEEGDEAVVQAPGGERTYEIVEVRYE
HCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure 
SKIPLTIRGAEKLREELHRLKHEDRPRVIQAIAEAREHGDLKENAEYHAAREQQSFIEG
CCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIQEIEGKLSNAQIIDPAAVQAAPKIVFGATVALEDTDTEETVTYQIVGEDEADIKQHLI
HHHHHHCCCCCCEEECHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHH
SVNSPIARALIGKEEGDEAVVQAPGGERTYEIVEVRYE
HCCCHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA