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The map label for this gene is rlmE

Identifier: 121998550

GI number: 121998550

Start: 1945245

End: 1945883

Strand: Reverse

Name: rlmE

Synonym: Hhal_1771

Alternate gene names: 121998550

Gene position: 1945883-1945245 (Counterclockwise)

Preceding gene: 121998552

Following gene: 121998549

Centisome position: 72.65

GC content: 67.61

Gene sequence:

>639_bases
ATGGCAACTTGTAGGCGGCGTCGGCGAGGGTGCAACTCGCAGGCTCGGCGGAGCCGGCACGAGAGCGACCCCTTTGTCCG
CCGTGCCCGGGCCGAGGGGTGGCGCTCCAGGGCGGCGCTCAAGCTGGAGGCCATCGATCAGCGTGACGGGCTCTTTCGGC
CCGGGCAGGTGGTGGTCGACCTGGGGGCGGCGCCCGGCGGCTGGTCGCAGCTGGTGGCCCCGAAGGTCGGCTCGGCCGGG
CGGGTGGTCGCCATCGACTTGCTGGAGATGGATCCGCTGCCTGGGGTCACGTTCCTGCACGCCGACTTCTCCACGGATGA
GGGGTTACGCGCCGTCGAGAGGGCCCTGGAGGGCCGCCCGGTGGATATCGTGCTCTCGGATATGGCGCCGAATCTGACCG
GTCACAACGCAGTGGACCAGCCGGCAGCCATGGGGCTGGCGGAACTTGCCGCCGACTTTGCTGGTCAATTCCTGCATAAG
AATGGCGACTTTCTAGTTAAGGTCTTCCACGGCGAGGGATTCGACGCGTTCCGCGGGGACCTGACCCGCCGCTTCTCCCG
CGTACTTTCCCGTAAGCCGGATGCCTCCAGGTCGGGGAGTCGGGAGGTTTACCTTCTGGCTCGTGGTCCGACGGTATAG

Upstream 100 bases:

>100_bases
GAAGTCGTCGGCGTGCTGGCGGCGGCCTGCCTGGGGGGCGGTTGGGTGGCGTTGCGCGTGCGCGTGCTGGCGCGTCGCAG
ACTGGCGGAGGGCCGTGAGC

Downstream 100 bases:

>100_bases
TAAGGTGCCTGTCAGGTGCCGGATTCAGGCAGATTGAGGTGACGCCGTGAGCGACATGGCCAAGAATTTGATCCTTTGGG
TCATCATCGCCGTCGTGCTG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MATCRRRRRGCNSQARRSRHESDPFVRRARAEGWRSRAALKLEAIDQRDGLFRPGQVVVDLGAAPGGWSQLVAPKVGSAG
RVVAIDLLEMDPLPGVTFLHADFSTDEGLRAVERALEGRPVDIVLSDMAPNLTGHNAVDQPAAMGLAELAADFAGQFLHK
NGDFLVKVFHGEGFDAFRGDLTRRFSRVLSRKPDASRSGSREVYLLARGPTV

Sequences:

>Translated_212_residues
MATCRRRRRGCNSQARRSRHESDPFVRRARAEGWRSRAALKLEAIDQRDGLFRPGQVVVDLGAAPGGWSQLVAPKVGSAG
RVVAIDLLEMDPLPGVTFLHADFSTDEGLRAVERALEGRPVDIVLSDMAPNLTGHNAVDQPAAMGLAELAADFAGQFLHK
NGDFLVKVFHGEGFDAFRGDLTRRFSRVLSRKPDASRSGSREVYLLARGPTV
>Mature_211_residues
ATCRRRRRGCNSQARRSRHESDPFVRRARAEGWRSRAALKLEAIDQRDGLFRPGQVVVDLGAAPGGWSQLVAPKVGSAGR
VVAIDLLEMDPLPGVTFLHADFSTDEGLRAVERALEGRPVDIVLSDMAPNLTGHNAVDQPAAMGLAELAADFAGQFLHKN
GDFLVKVFHGEGFDAFRGDLTRRFSRVLSRKPDASRSGSREVYLLARGPTV

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=199, Percent_Identity=39.6984924623116, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=36.734693877551, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=36.734693877551, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=36.734693877551, Blast_Score=127, Evalue=5e-30,
Organism=Homo sapiens, GI194097365, Length=187, Percent_Identity=33.6898395721925, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=48.2758620689655, Blast_Score=195, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553474, Length=199, Percent_Identity=36.6834170854271, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=32.3076923076923, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=32.8042328042328, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=32.8042328042328, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=32.8042328042328, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=204, Percent_Identity=32.3529411764706, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=198, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=282, Percent_Identity=25.886524822695, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356387, Length=210, Percent_Identity=35.7142857142857, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=31.6326530612245, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648639, Length=201, Percent_Identity=33.8308457711443, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24647580, Length=202, Percent_Identity=30.1980198019802, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_HALHL (A1WXX3)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003337.1
- ProteinModelPortal:   A1WXX3
- SMR:   A1WXX3
- STRING:   A1WXX3
- GeneID:   4710934
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1771
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1WXX3
- BioCyc:   HHAL349124:HHAL_1771-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23159; Mature: 23027

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: ACT_SITE 167-167 BINDING 66-66 BINDING 68-68 BINDING 86-86 BINDING 102-102 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATCRRRRRGCNSQARRSRHESDPFVRRARAEGWRSRAALKLEAIDQRDGLFRPGQVVVD
CCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
LGAAPGGWSQLVAPKVGSAGRVVAIDLLEMDPLPGVTFLHADFSTDEGLRAVERALEGRP
CCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
VDIVLSDMAPNLTGHNAVDQPAAMGLAELAADFAGQFLHKNGDFLVKVFHGEGFDAFRGD
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LTRRFSRVLSRKPDASRSGSREVYLLARGPTV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
ATCRRRRRGCNSQARRSRHESDPFVRRARAEGWRSRAALKLEAIDQRDGLFRPGQVVVD
CCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEE
LGAAPGGWSQLVAPKVGSAGRVVAIDLLEMDPLPGVTFLHADFSTDEGLRAVERALEGRP
CCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
VDIVLSDMAPNLTGHNAVDQPAAMGLAELAADFAGQFLHKNGDFLVKVFHGEGFDAFRGD
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LTRRFSRVLSRKPDASRSGSREVYLLARGPTV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA