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The map label for this gene is pncA [H]

Identifier: 121998523

GI number: 121998523

Start: 1915075

End: 1915725

Strand: Reverse

Name: pncA [H]

Synonym: Hhal_1744

Alternate gene names: 121998523

Gene position: 1915725-1915075 (Counterclockwise)

Preceding gene: 121998524

Following gene: 121998519

Centisome position: 71.52

GC content: 68.97

Gene sequence:

>651_bases
GTGGTTGAAGCCAAGCTAAGAGACGCGGAGCACGCCGCGTTGCTCGTCGTCGACGTCCAGCCGGACTTTATGCCCGGCGG
TGCGTTGCCGACCCAGGGCGGCGACGAAGTGGTCCCCCCCATTGCCCGCCTGCTGGAGCGGGCGCCCAGCCGCTACGTGG
TGGCGACCCAGGACTGGCACCCACCGGGGCACATCTCCTTTGCCTCCAGCCATCCAGGGTGTTCCCCCTTCGAGGTGATC
GAGCTCCACGGCGTCGACCAGGTGCTCTGGCCCGATCACTGTGTCCAGGAGACCCGCGGTGCAGCCCTGCACGACGGGGT
CCCATGGCGCCATGCAGACCTCATCCTGCGTAAGGCCACCGATCCGCTGGTCGACTCGTACAGCGCGTTCCGCGAGAACT
TCGCCCCGGATGGACGGCGGCCGTCGACGGGTCTGACCGGCTACCTGCGCGAACTCGGTGTCGAGCAGCTCTACGTCTGC
GGCCTGGCGAGGGACTACTGCGCACTGTGGAGCGCGGAGGACGGGGCCCAGGCGGGCTTCGGGGTGCACTTCCTGTGGGA
TCTGACCCGTCCTGTGGATGCCAGCAACGACGCCTCGGTGCAGCGGACGCTGCAGGCGGCCGGGGTGGAGATCGTCACCG
GGGTGCCCTGA

Upstream 100 bases:

>100_bases
AACGGCCGCCGGACCACTGCAATCGCTGCGGTGTCGGCTTGCTGCCGGCGCCGCGGCGGCTACCCTGAAGGGGGTGCCGA
CCGATCGTCGGGAGGTTGCG

Downstream 100 bases:

>100_bases
GCGGTTCAGGCAAAGAAGCCGTGCAGGTGCCAACCGTAGGGCGGGCGACGGTCGCGATAGACCCAGAGCCGGCTGCCGAC
CGGGGCGCGCGCGACGTAGT

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MVEAKLRDAEHAALLVVDVQPDFMPGGALPTQGGDEVVPPIARLLERAPSRYVVATQDWHPPGHISFASSHPGCSPFEVI
ELHGVDQVLWPDHCVQETRGAALHDGVPWRHADLILRKATDPLVDSYSAFRENFAPDGRRPSTGLTGYLRELGVEQLYVC
GLARDYCALWSAEDGAQAGFGVHFLWDLTRPVDASNDASVQRTLQAAGVEIVTGVP

Sequences:

>Translated_216_residues
MVEAKLRDAEHAALLVVDVQPDFMPGGALPTQGGDEVVPPIARLLERAPSRYVVATQDWHPPGHISFASSHPGCSPFEVI
ELHGVDQVLWPDHCVQETRGAALHDGVPWRHADLILRKATDPLVDSYSAFRENFAPDGRRPSTGLTGYLRELGVEQLYVC
GLARDYCALWSAEDGAQAGFGVHFLWDLTRPVDASNDASVQRTLQAAGVEIVTGVP
>Mature_216_residues
MVEAKLRDAEHAALLVVDVQPDFMPGGALPTQGGDEVVPPIARLLERAPSRYVVATQDWHPPGHISFASSHPGCSPFEVI
ELHGVDQVLWPDHCVQETRGAALHDGVPWRHADLILRKATDPLVDSYSAFRENFAPDGRRPSTGLTGYLRELGVEQLYVC
GLARDYCALWSAEDGAQAGFGVHFLWDLTRPVDASNDASVQRTLQAAGVEIVTGVP

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=205, Percent_Identity=41.9512195121951, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543130, Length=201, Percent_Identity=31.8407960199005, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71999339, Length=223, Percent_Identity=29.5964125560538, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=224, Percent_Identity=32.5892857142857, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648247, Length=221, Percent_Identity=31.6742081447964, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23467; Mature: 23467

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEAKLRDAEHAALLVVDVQPDFMPGGALPTQGGDEVVPPIARLLERAPSRYVVATQDWH
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCC
PPGHISFASSHPGCSPFEVIELHGVDQVLWPDHCVQETRGAALHDGVPWRHADLILRKAT
CCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
DPLVDSYSAFRENFAPDGRRPSTGLTGYLRELGVEQLYVCGLARDYCALWSAEDGAQAGF
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GVHFLWDLTRPVDASNDASVQRTLQAAGVEIVTGVP
CEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCC
>Mature Secondary Structure
MVEAKLRDAEHAALLVVDVQPDFMPGGALPTQGGDEVVPPIARLLERAPSRYVVATQDWH
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCC
PPGHISFASSHPGCSPFEVIELHGVDQVLWPDHCVQETRGAALHDGVPWRHADLILRKAT
CCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
DPLVDSYSAFRENFAPDGRRPSTGLTGYLRELGVEQLYVCGLARDYCALWSAEDGAQAGF
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GVHFLWDLTRPVDASNDASVQRTLQAAGVEIVTGVP
CEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]