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The map label for this gene is relA [H]

Identifier: 121998519

GI number: 121998519

Start: 1909824

End: 1912034

Strand: Reverse

Name: relA [H]

Synonym: Hhal_1740

Alternate gene names: 121998519

Gene position: 1912034-1909824 (Counterclockwise)

Preceding gene: 121998523

Following gene: 121998518

Centisome position: 71.39

GC content: 67.16

Gene sequence:

>2211_bases
ATGGTCCAGTCCGCCCTAGCCGATATCGACGACGTCACCGCCCTGGAGCCCGCAGTGTGGCTGCATCGCCTGCCGGTGTC
GGTCAGTGACGCCGACCGTGCACTGCTCGAGCAGGCCTGGGAGCGCGCACGGGCCGGCTACGGTGAGCGGCTGCGCGACT
CGGGCGAGCCGTATTTCGCCCACGCCGTCTACACCGCCACCATCCTGGCCGAGCTGGGTCTGGACGCCGCGACCCTGGCG
GCGGCGCTGATCCACGATCTGCCGGAGCTGCAGGAAGGCTCCATCGCCGGTCTGCGCAAGCACCTGGGAGGTGATATCGC
CGAGCTCGCCCAGGGGGTGTGCCGTATGCACGGCATTGGTCGGTTCCGCGATTCGGCCGAGCTGGAGGCAGCGATCCAGG
GGGAGCGGGTCGAGGGGCTGCGCAAGATGCTGCTGGCTATGGCCCGCGACGTCCGCGTGGTGTTCATCGCCCTGGCCGAG
CGGTTGCACGATCTGCGCAGCTGTCGAGGTCTCGACGAGGAGGGGCGCATGCGCCTGGCCCGCGAGACCCGGGATATCTA
TGCACCCTTGGCCAACCGGTTGGGGATCTGGCAGCTCAAGTGGGAGCTGGAAGACCTGGCTTTTCGGCATCTGGAGCCCG
AGGCCTACATGGATCTGGCCAAACGCCTGGCCGAGCGCCGGCGCGACCGCGAGGCCTATATCGAGCAGGTCTGCAGCCAG
CTGCAGGCGGCCATGGACGAGGCCGGCCTCAAGGCCGAGGTCTACGGACGTCCCAAGCACATCTACAGCATCTATCGGAA
GATGCAGCGCAAGGGGCTGCGTTTCGACGATCTCTACGATCTGCGCGCAGTGCGCATCCTCGTCGATGACGAGCGCACCT
GCTACGCCGCCCTGGGGATCGTCCACGGCCTGTGGACGCCGATCCCGCGGGAGTTCGATGACTACATCGCAACCCCCAAG
GAGAACAACTACCGTTCGCTGCATACCGCGGTGGCCGGCCCCGAGGGCAAAGCGGTGGAGGTGCAGATCCGTACCCACGA
GATGCACGCCGAGGCCGAGCTGGGCATTGCCGCCCACTGGCGCTACAAGGAGGGCGGCAAGCAGGATGCCGAATTCGAGC
AGAAGATCGCCTGGCTGCGTCAGATCCTAGAGTGGGGGCGAGAGGATGAAGACGACGAGGGGGATTTCGTCGATCGTGTC
CGTGCCGAGGTCTTCGCCGATCGGGTCTACGCCCTGACTCCCGGCGGCGACGTGATCGATCTGCCGCGCGGGGCGACAGC
GCTGGATTTTGCTTATCACATCCATACCGGCCTCGGGCACCGCTGCCGCGGGGCCAAGATCAATAACCGGATCGCGCCGC
TGACCCGTGCCCTGGACAACGGGGATCGAGTCGAGATCCTGACCAGCCGCGAGGAGCGACCGAGCCGCGACTGGATCAAT
CCGGAGCTGGGCTATCTCTACACCAACCGGGCCCGCACCAAGGTGCGCACCTGGTTCCGTCAGCGCGATCACGACAAGAA
CGTGGCCGCCGGCCGCCAGGCCCTGGACAAGGAGTTGCGCCGTCTAGGTCTGGAGGACGTCAGCACCGAAGAGGTGGTCC
GGCGCAGCCGCTATACCCGGGTGGAGCTGTTCCTCTCGGCCCTGGGTCGCGGCGAGGTGACCCCCGGGCAGATCGCAGCC
CTGTTGCGCGACCGGTTGCTGCCCCAGCAGCCCGAACCCGCCTCTGCGCCCGAGCGGCCCCGCGCTACCGAGAAACCCGG
TGGCGACGGCGATATCACCGTGGAGGGGGTCGGCAACCTGTTGACTCGCATGGCCCGTTGCTGCAGTCCGGCCCCGGGGG
ATCCGATTATCGGGTTCATTACCCGGGGGGCGGGGGTAACGATCCACCGCCAGGATTGCCGCAACGTCGAGCAGCTGCGT
GAGCGCGAGCCAGAGCGTCTGCTCGACGTCAACTGGCGTACGGCCCCTGCCGGTCGGTACCCGGTGCGCATCCACGTCCG
TACCGGCGTGCTGGACGGGCCGTTGAGCGAGATCACCCGCCTTTGTGGCCACGAAGGGGTACGCCTGGATGGGGTACAGA
CCCATGCGGAGCCGGCGGACGCCTACCGCATCGATATCGACGTGCAGGTCAGTGACGTCCAGCAGCTCTCGCGGTTGATG
GCCAAGCTTTCATCCAGTGAGCGGGTGGAGCGCGTATGGCGCGGCACATAA

Upstream 100 bases:

>100_bases
AGCGGCCGGCGGTGTGTCTGTTGTCCCTTGTCACGTTGCCGCTGCAGCCTGAAACTCTCCTACCCTGAACGTGAACCCTC
AACTTGAACCCCCGAGGCCC

Downstream 100 bases:

>100_bases
GGGGCGGCTAATATAGCCGTGGCCATCCACGGCCCCGGCCAGGAGTCACCATGCGTCCCGCTTACCAGATCCTCTTCGCT
GCCCTGCTGATTGCCGGGGT

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MVQSALADIDDVTALEPAVWLHRLPVSVSDADRALLEQAWERARAGYGERLRDSGEPYFAHAVYTATILAELGLDAATLA
AALIHDLPELQEGSIAGLRKHLGGDIAELAQGVCRMHGIGRFRDSAELEAAIQGERVEGLRKMLLAMARDVRVVFIALAE
RLHDLRSCRGLDEEGRMRLARETRDIYAPLANRLGIWQLKWELEDLAFRHLEPEAYMDLAKRLAERRRDREAYIEQVCSQ
LQAAMDEAGLKAEVYGRPKHIYSIYRKMQRKGLRFDDLYDLRAVRILVDDERTCYAALGIVHGLWTPIPREFDDYIATPK
ENNYRSLHTAVAGPEGKAVEVQIRTHEMHAEAELGIAAHWRYKEGGKQDAEFEQKIAWLRQILEWGREDEDDEGDFVDRV
RAEVFADRVYALTPGGDVIDLPRGATALDFAYHIHTGLGHRCRGAKINNRIAPLTRALDNGDRVEILTSREERPSRDWIN
PELGYLYTNRARTKVRTWFRQRDHDKNVAAGRQALDKELRRLGLEDVSTEEVVRRSRYTRVELFLSALGRGEVTPGQIAA
LLRDRLLPQQPEPASAPERPRATEKPGGDGDITVEGVGNLLTRMARCCSPAPGDPIIGFITRGAGVTIHRQDCRNVEQLR
EREPERLLDVNWRTAPAGRYPVRIHVRTGVLDGPLSEITRLCGHEGVRLDGVQTHAEPADAYRIDIDVQVSDVQQLSRLM
AKLSSSERVERVWRGT

Sequences:

>Translated_736_residues
MVQSALADIDDVTALEPAVWLHRLPVSVSDADRALLEQAWERARAGYGERLRDSGEPYFAHAVYTATILAELGLDAATLA
AALIHDLPELQEGSIAGLRKHLGGDIAELAQGVCRMHGIGRFRDSAELEAAIQGERVEGLRKMLLAMARDVRVVFIALAE
RLHDLRSCRGLDEEGRMRLARETRDIYAPLANRLGIWQLKWELEDLAFRHLEPEAYMDLAKRLAERRRDREAYIEQVCSQ
LQAAMDEAGLKAEVYGRPKHIYSIYRKMQRKGLRFDDLYDLRAVRILVDDERTCYAALGIVHGLWTPIPREFDDYIATPK
ENNYRSLHTAVAGPEGKAVEVQIRTHEMHAEAELGIAAHWRYKEGGKQDAEFEQKIAWLRQILEWGREDEDDEGDFVDRV
RAEVFADRVYALTPGGDVIDLPRGATALDFAYHIHTGLGHRCRGAKINNRIAPLTRALDNGDRVEILTSREERPSRDWIN
PELGYLYTNRARTKVRTWFRQRDHDKNVAAGRQALDKELRRLGLEDVSTEEVVRRSRYTRVELFLSALGRGEVTPGQIAA
LLRDRLLPQQPEPASAPERPRATEKPGGDGDITVEGVGNLLTRMARCCSPAPGDPIIGFITRGAGVTIHRQDCRNVEQLR
EREPERLLDVNWRTAPAGRYPVRIHVRTGVLDGPLSEITRLCGHEGVRLDGVQTHAEPADAYRIDIDVQVSDVQQLSRLM
AKLSSSERVERVWRGT
>Mature_736_residues
MVQSALADIDDVTALEPAVWLHRLPVSVSDADRALLEQAWERARAGYGERLRDSGEPYFAHAVYTATILAELGLDAATLA
AALIHDLPELQEGSIAGLRKHLGGDIAELAQGVCRMHGIGRFRDSAELEAAIQGERVEGLRKMLLAMARDVRVVFIALAE
RLHDLRSCRGLDEEGRMRLARETRDIYAPLANRLGIWQLKWELEDLAFRHLEPEAYMDLAKRLAERRRDREAYIEQVCSQ
LQAAMDEAGLKAEVYGRPKHIYSIYRKMQRKGLRFDDLYDLRAVRILVDDERTCYAALGIVHGLWTPIPREFDDYIATPK
ENNYRSLHTAVAGPEGKAVEVQIRTHEMHAEAELGIAAHWRYKEGGKQDAEFEQKIAWLRQILEWGREDEDDEGDFVDRV
RAEVFADRVYALTPGGDVIDLPRGATALDFAYHIHTGLGHRCRGAKINNRIAPLTRALDNGDRVEILTSREERPSRDWIN
PELGYLYTNRARTKVRTWFRQRDHDKNVAAGRQALDKELRRLGLEDVSTEEVVRRSRYTRVELFLSALGRGEVTPGQIAA
LLRDRLLPQQPEPASAPERPRATEKPGGDGDITVEGVGNLLTRMARCCSPAPGDPIIGFITRGAGVTIHRQDCRNVEQLR
EREPERLLDVNWRTAPAGRYPVRIHVRTGVLDGPLSEITRLCGHEGVRLDGVQTHAEPADAYRIDIDVQVSDVQQLSRLM
AKLSSSERVERVWRGT

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=740, Percent_Identity=43.3783783783784, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=35.9264497878359, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83190; Mature: 83190

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSALADIDDVTALEPAVWLHRLPVSVSDADRALLEQAWERARAGYGERLRDSGEPYFA
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEH
HAVYTATILAELGLDAATLAAALIHDLPELQEGSIAGLRKHLGGDIAELAQGVCRMHGIG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
RFRDSAELEAAIQGERVEGLRKMLLAMARDVRVVFIALAERLHDLRSCRGLDEEGRMRLA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RETRDIYAPLANRLGIWQLKWELEDLAFRHLEPEAYMDLAKRLAERRRDREAYIEQVCSQ
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAAMDEAGLKAEVYGRPKHIYSIYRKMQRKGLRFDDLYDLRAVRILVDDERTCYAALGI
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEECCCCHHHHHHHH
VHGLWTPIPREFDDYIATPKENNYRSLHTAVAGPEGKAVEVQIRTHEMHAEAELGIAAHW
HHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEHHHHHHHCCCEEEEE
RYKEGGKQDAEFEQKIAWLRQILEWGREDEDDEGDFVDRVRAEVFADRVYALTPGGDVID
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEE
LPRGATALDFAYHIHTGLGHRCRGAKINNRIAPLTRALDNGDRVEILTSREERPSRDWIN
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
PELGYLYTNRARTKVRTWFRQRDHDKNVAAGRQALDKELRRLGLEDVSTEEVVRRSRYTR
CCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VELFLSALGRGEVTPGQIAALLRDRLLPQQPEPASAPERPRATEKPGGDGDITVEGVGNL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
LTRMARCCSPAPGDPIIGFITRGAGVTIHRQDCRNVEQLREREPERLLDVNWRTAPAGRY
HHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHCCCHHHEECCCCCCCCCCC
PVRIHVRTGVLDGPLSEITRLCGHEGVRLDGVQTHAEPADAYRIDIDVQVSDVQQLSRLM
CEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
AKLSSSERVERVWRGT
HHHCCHHHHHHHHCCC
>Mature Secondary Structure
MVQSALADIDDVTALEPAVWLHRLPVSVSDADRALLEQAWERARAGYGERLRDSGEPYFA
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEH
HAVYTATILAELGLDAATLAAALIHDLPELQEGSIAGLRKHLGGDIAELAQGVCRMHGIG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
RFRDSAELEAAIQGERVEGLRKMLLAMARDVRVVFIALAERLHDLRSCRGLDEEGRMRLA
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RETRDIYAPLANRLGIWQLKWELEDLAFRHLEPEAYMDLAKRLAERRRDREAYIEQVCSQ
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAAMDEAGLKAEVYGRPKHIYSIYRKMQRKGLRFDDLYDLRAVRILVDDERTCYAALGI
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHCEEEEEEECCCCHHHHHHHH
VHGLWTPIPREFDDYIATPKENNYRSLHTAVAGPEGKAVEVQIRTHEMHAEAELGIAAHW
HHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEHHHHHHHCCCEEEEE
RYKEGGKQDAEFEQKIAWLRQILEWGREDEDDEGDFVDRVRAEVFADRVYALTPGGDVID
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEE
LPRGATALDFAYHIHTGLGHRCRGAKINNRIAPLTRALDNGDRVEILTSREERPSRDWIN
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
PELGYLYTNRARTKVRTWFRQRDHDKNVAAGRQALDKELRRLGLEDVSTEEVVRRSRYTR
CCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VELFLSALGRGEVTPGQIAALLRDRLLPQQPEPASAPERPRATEKPGGDGDITVEGVGNL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
LTRMARCCSPAPGDPIIGFITRGAGVTIHRQDCRNVEQLREREPERLLDVNWRTAPAGRY
HHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHCCCHHHEECCCCCCCCCCC
PVRIHVRTGVLDGPLSEITRLCGHEGVRLDGVQTHAEPADAYRIDIDVQVSDVQQLSRLM
CEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
AKLSSSERVERVWRGT
HHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]