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The map label for this gene is metK [H]

Identifier: 121998455

GI number: 121998455

Start: 1828733

End: 1829899

Strand: Reverse

Name: metK [H]

Synonym: Hhal_1676

Alternate gene names: 121998455

Gene position: 1829899-1828733 (Counterclockwise)

Preceding gene: 121998456

Following gene: 121998454

Centisome position: 68.32

GC content: 65.38

Gene sequence:

>1167_bases
ATGAGCAAGCGCTACCTCTTTACGTCTGAGTCGGTGTCCGAGGGCCACCCGGACAAGATGGCCGACGCGATCTCCGATGC
CCTGCTGGACGAGTTCCTGCGCCAGGATCCGAAGTCCCGCGTGGCGGCTGAGACCATGATCCAGACCGGCATGGTCGTGG
TAGCTGGCGAGATCAAGAGCACGGCCAAGGTCAACGTTGAGCCGCTGGTTCGCGACGTGGTCCGTGAGATCGGCTACACC
AGCTCGGACATGGGCTTCGACGCCGACACCTGCGCGGTGCTCAACGCGCTGGGCGAGCAGTCCCCGGACATCAACCAGGG
CGTCGATCGCGAGGAGGAAGAGGAGCAGGGCGCTGGTGACCAGGGCCTGATGTTCGGCTACGCCACCAATGAGACCGATG
TGCTCATGCCGGCGGCCATCCACTACTCCCACCTGCTGGTCAAGCGCCAGGCCGAGGTGCGCAAGAACGGCAAGCTGCCG
TGGCTGCGCCCCGACGCGAAGAGCCAGGTCACCTTCCAGTACGAAGGCGACAAGATCGTTGGCTGTGACGCCGTGGTGCT
CTCCACCCAGCACGACGAGACCGTGGATCAGAAGACGGTCCACGAGGGCGTGATGGAGGAGATCATCAAGCCCGTACTGG
GTGACACCGGCTGGCTGACCAACGACACCAAGTATCACATCAACCCGACCGGGCGCTTCGTCACCGGCGGCCCGCTGGGT
GATTGCGGCCTGACCGGCCGGAAGATCATCGTCGACACCTACGGCGGCATGGGCCGTCACGGCGGTGGTGCCTTCTCCGG
CAAGGATCCGTCCAAGGTCGACCGCTCGGCTGCGTACGTCGGTCGCTACGTCGCCAAGAACGTGGTCGCCGCCGGCCTGG
CTGACCGCTGCGAGGTGCAGCTCTCCTACGCCATCGGCGTTGCCGAGCCGACCTCCGTCAACGTCGAGACCTTCGGCACC
GGCAAGATCGACGAGGAAGTGATCAACAAGGTGGTCCGCGAGAACTTCGACCTGCGTCCGTACGGCATCCTGAAGATGCT
GGGCCTGGAGCAGCCGATCTACCGCCCGACCGCCGCCTACGGCCACTTTGGCCGCGAGGACATCGACGTGCCGTGGGAGC
GCACCGACCGTGCTGCCGCCCTCAAGGACGCCGCTGGCCTCAAGTAA

Upstream 100 bases:

>100_bases
TAACCCCCGCGTATTCACCCATGGATTCCGGGCCGCGCGCGGCTGCGGCCGCGCCGCCGGAGTTGTATAACCTGAGAATC
CTTAGCGAGAAGGAGTAACC

Downstream 100 bases:

>100_bases
CCGAATACGCAAGGAGTAACCCGAAAGCATGAGTAATCAAGACTACAAGGTTGCGGATATCTCCCTGGCCGACTGGGGCC
GTAAGGAGATCAAGATCGCC

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSKRYLFTSESVSEGHPDKMADAISDALLDEFLRQDPKSRVAAETMIQTGMVVVAGEIKSTAKVNVEPLVRDVVREIGYT
SSDMGFDADTCAVLNALGEQSPDINQGVDREEEEEQGAGDQGLMFGYATNETDVLMPAAIHYSHLLVKRQAEVRKNGKLP
WLRPDAKSQVTFQYEGDKIVGCDAVVLSTQHDETVDQKTVHEGVMEEIIKPVLGDTGWLTNDTKYHINPTGRFVTGGPLG
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYVGRYVAKNVVAAGLADRCEVQLSYAIGVAEPTSVNVETFGT
GKIDEEVINKVVRENFDLRPYGILKMLGLEQPIYRPTAAYGHFGREDIDVPWERTDRAAALKDAAGLK

Sequences:

>Translated_388_residues
MSKRYLFTSESVSEGHPDKMADAISDALLDEFLRQDPKSRVAAETMIQTGMVVVAGEIKSTAKVNVEPLVRDVVREIGYT
SSDMGFDADTCAVLNALGEQSPDINQGVDREEEEEQGAGDQGLMFGYATNETDVLMPAAIHYSHLLVKRQAEVRKNGKLP
WLRPDAKSQVTFQYEGDKIVGCDAVVLSTQHDETVDQKTVHEGVMEEIIKPVLGDTGWLTNDTKYHINPTGRFVTGGPLG
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYVGRYVAKNVVAAGLADRCEVQLSYAIGVAEPTSVNVETFGT
GKIDEEVINKVVRENFDLRPYGILKMLGLEQPIYRPTAAYGHFGREDIDVPWERTDRAAALKDAAGLK
>Mature_387_residues
SKRYLFTSESVSEGHPDKMADAISDALLDEFLRQDPKSRVAAETMIQTGMVVVAGEIKSTAKVNVEPLVRDVVREIGYTS
SDMGFDADTCAVLNALGEQSPDINQGVDREEEEEQGAGDQGLMFGYATNETDVLMPAAIHYSHLLVKRQAEVRKNGKLPW
LRPDAKSQVTFQYEGDKIVGCDAVVLSTQHDETVDQKTVHEGVMEEIIKPVLGDTGWLTNDTKYHINPTGRFVTGGPLGD
CGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYVGRYVAKNVVAAGLADRCEVQLSYAIGVAEPTSVNVETFGTG
KIDEEVINKVVRENFDLRPYGILKMLGLEQPIYRPTAAYGHFGREDIDVPWERTDRAAALKDAAGLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=55.6149732620321, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=55.0802139037433, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=74.025974025974, Blast_Score=596, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI25145633, Length=389, Percent_Identity=54.7557840616967, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=54.5212765957447, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17551082, Length=379, Percent_Identity=54.353562005277, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=54.2553191489362, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=48.6702127659575, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=52.6627218934911, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=52.5835866261398, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=51.9867549668874, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=57.8313253012048, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=53.0343007915567, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323209, Length=380, Percent_Identity=53.1578947368421, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=53.2085561497326, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=52.4064171122995, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=52.4064171122995, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=50.1333333333333, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=55.0847457627119, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42246; Mature: 42115

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYLFTSESVSEGHPDKMADAISDALLDEFLRQDPKSRVAAETMIQTGMVVVAGEIKS
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCC
TAKVNVEPLVRDVVREIGYTSSDMGFDADTCAVLNALGEQSPDINQGVDREEEEEQGAGD
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCC
QGLMFGYATNETDVLMPAAIHYSHLLVKRQAEVRKNGKLPWLRPDAKSQVTFQYEGDKIV
CCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
GCDAVVLSTQHDETVDQKTVHEGVMEEIIKPVLGDTGWLTNDTKYHINPTGRFVTGGPLG
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYVGRYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEE
LSYAIGVAEPTSVNVETFGTGKIDEEVINKVVRENFDLRPYGILKMLGLEQPIYRPTAAY
EEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHH
GHFGREDIDVPWERTDRAAALKDAAGLK
CCCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SKRYLFTSESVSEGHPDKMADAISDALLDEFLRQDPKSRVAAETMIQTGMVVVAGEIKS
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCC
TAKVNVEPLVRDVVREIGYTSSDMGFDADTCAVLNALGEQSPDINQGVDREEEEEQGAGD
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCC
QGLMFGYATNETDVLMPAAIHYSHLLVKRQAEVRKNGKLPWLRPDAKSQVTFQYEGDKIV
CCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
GCDAVVLSTQHDETVDQKTVHEGVMEEIIKPVLGDTGWLTNDTKYHINPTGRFVTGGPLG
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYVGRYVAKNVVAAGLADRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEE
LSYAIGVAEPTSVNVETFGTGKIDEEVINKVVRENFDLRPYGILKMLGLEQPIYRPTAAY
EEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHH
GHFGREDIDVPWERTDRAAALKDAAGLK
CCCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]