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The map label for this gene is trpB2 [H]

Identifier: 121998421

GI number: 121998421

Start: 1792316

End: 1793680

Strand: Reverse

Name: trpB2 [H]

Synonym: Hhal_1642

Alternate gene names: 121998421

Gene position: 1793680-1792316 (Counterclockwise)

Preceding gene: 121998423

Following gene: 121998420

Centisome position: 66.97

GC content: 67.25

Gene sequence:

>1365_bases
ATGAGTGAGACCGTCAAGTATGTCCTCGATGAGGCGCGGATCCCGCGCGCCTGGTACAACATCAGCGCCGATCTGCCCGC
CCCGCTGGCGCCGGTGCTCCACCCGGCAACCCGGGAGCCGGTTTCGGCGGACGACCTGGCGCCGCTCTTCCCGCGCGGAG
TGATTGAGCAGGAGCTGACTACGGAGCGGGAGGTCGAGATCCCGGAGCCGGTTCGCAACGCCTATCGGCAGTGGCGGCCG
GCGCCGCTTTACCGGGCCCGGCGCCTGGAGAAGGCCCTCGATACCCCGGCGCGCATCTATTACAAGTACGAGGGCGTCAG
CCCGGCCGGGAGTCACAAGCCGAATACCGCGCTCCCGCAGGCCTTCTACAACCACGCCGAGGGGGTGCGCCGCATCACCA
CCGAGACTGGGGCCGGTCAGTGGGGGTCGTCCCTGGCGCTGGCCGGCGCCATGTTCGATCTCGACATCGAGGTCTTCATG
GTGGGGGTCAGTTATAAGCAGAAACCCTACCGGCGTGCGTTCATGGAGAGCTTCGGTGCGCGGTGCATTGCCAGCCCGAG
CCAAGAGACCGCCTTTGGCCGCTCGGTCTTGGCGGACAACCCGGCCAGCACCGGGAGTCTGGGCATCGCCATCGGCGAGG
CGGTGGAGCTGGCCGCGCAGCGCGACGACACCAAGTACGCGCTGGGCAGCGTGCTCAACCACGTCCTGCTACATCAGACC
GTGACGGGTGTCGAGGCGATGGAGCAGATGGCCATGGCCGACGATTACCCCGACACGGTCATTGCCTGTACCGGGGGTGG
CAGCAACTTCGCGGGGATCGTTTTCCCGTTCCTCGGCGCCGCCCTGCGCGGCGGCCCGAGCGTCGAGGCCATCGCCGTGG
AGCCGCTGGCCTGTCCGACCCTAACCCGGGGGCAGTTTGCCTACGATTACGGTGACTCGGCGCAGTTGACGCCGCTGACC
AAGATGCACACCCTTGGCTCCGGTTTCATGCCCCCGGGGTTTCACGCCGGGGGGCTGCGTTACCACGGCATGGCCCCCCA
GATCAGCCATTTGAAGGACCTCGGCTACCTCGACGCGCGGGCCTACGATCAGCTCGACTGCTTTGCTGCCGGTCTGGAAT
TTGCGCGGGCCGAGGGCATCATCCCCGCCCCCGAGGCCAATCACGCGGTCAAGGCGGTGATCGAGGAGGCCCAGGGGTGC
CGCGAACGTGGCGAGTCGAAGGCGATCCTGTTCAACCTTTGCGGCCACGGTAATTTTGACATGCAGGCCTATACGGATTA
CCTGGCGGGCAACCTCAGCGACCTCGGCGGCAGCCAGGTCGAGGCGGCCATGGCGCTGTCCGGACTGCCGTCGGTGGAGC
GTTGA

Upstream 100 bases:

>100_bases
CTAGCCGGCGGCGGCGTGTCGCGCCTGGTCAGCTTCCCCCCGTGGCCGGGCGCGGCTACCATAGCCGGCGTTCTGATCTG
GGCCAGCGCGAGAGCCAACC

Downstream 100 bases:

>100_bases
CGGCGGCTTGCCCATCCCACTTGCAGAGTTAAGGAGCGCAGCATGACCAACGAGATCCTCGAGGAGCGACAGCGGTTACT
CAAGGAACTCACCAAGTCGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSETVKYVLDEARIPRAWYNISADLPAPLAPVLHPATREPVSADDLAPLFPRGVIEQELTTEREVEIPEPVRNAYRQWRP
APLYRARRLEKALDTPARIYYKYEGVSPAGSHKPNTALPQAFYNHAEGVRRITTETGAGQWGSSLALAGAMFDLDIEVFM
VGVSYKQKPYRRAFMESFGARCIASPSQETAFGRSVLADNPASTGSLGIAIGEAVELAAQRDDTKYALGSVLNHVLLHQT
VTGVEAMEQMAMADDYPDTVIACTGGGSNFAGIVFPFLGAALRGGPSVEAIAVEPLACPTLTRGQFAYDYGDSAQLTPLT
KMHTLGSGFMPPGFHAGGLRYHGMAPQISHLKDLGYLDARAYDQLDCFAAGLEFARAEGIIPAPEANHAVKAVIEEAQGC
RERGESKAILFNLCGHGNFDMQAYTDYLAGNLSDLGGSQVEAAMALSGLPSVER

Sequences:

>Translated_454_residues
MSETVKYVLDEARIPRAWYNISADLPAPLAPVLHPATREPVSADDLAPLFPRGVIEQELTTEREVEIPEPVRNAYRQWRP
APLYRARRLEKALDTPARIYYKYEGVSPAGSHKPNTALPQAFYNHAEGVRRITTETGAGQWGSSLALAGAMFDLDIEVFM
VGVSYKQKPYRRAFMESFGARCIASPSQETAFGRSVLADNPASTGSLGIAIGEAVELAAQRDDTKYALGSVLNHVLLHQT
VTGVEAMEQMAMADDYPDTVIACTGGGSNFAGIVFPFLGAALRGGPSVEAIAVEPLACPTLTRGQFAYDYGDSAQLTPLT
KMHTLGSGFMPPGFHAGGLRYHGMAPQISHLKDLGYLDARAYDQLDCFAAGLEFARAEGIIPAPEANHAVKAVIEEAQGC
RERGESKAILFNLCGHGNFDMQAYTDYLAGNLSDLGGSQVEAAMALSGLPSVER
>Mature_453_residues
SETVKYVLDEARIPRAWYNISADLPAPLAPVLHPATREPVSADDLAPLFPRGVIEQELTTEREVEIPEPVRNAYRQWRPA
PLYRARRLEKALDTPARIYYKYEGVSPAGSHKPNTALPQAFYNHAEGVRRITTETGAGQWGSSLALAGAMFDLDIEVFMV
GVSYKQKPYRRAFMESFGARCIASPSQETAFGRSVLADNPASTGSLGIAIGEAVELAAQRDDTKYALGSVLNHVLLHQTV
TGVEAMEQMAMADDYPDTVIACTGGGSNFAGIVFPFLGAALRGGPSVEAIAVEPLACPTLTRGQFAYDYGDSAQLTPLTK
MHTLGSGFMPPGFHAGGLRYHGMAPQISHLKDLGYLDARAYDQLDCFAAGLEFARAEGIIPAPEANHAVKAVIEEAQGCR
ERGESKAILFNLCGHGNFDMQAYTDYLAGNLSDLGGSQVEAAMALSGLPSVER

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=363, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321412, Length=369, Percent_Identity=28.7262872628726, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 48888; Mature: 48757

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETVKYVLDEARIPRAWYNISADLPAPLAPVLHPATREPVSADDLAPLFPRGVIEQELT
CCHHHHHHHHHHCCCHHHEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHC
TEREVEIPEPVRNAYRQWRPAPLYRARRLEKALDTPARIYYKYEGVSPAGSHKPNTALPQ
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHH
AFYNHAEGVRRITTETGAGQWGSSLALAGAMFDLDIEVFMVGVSYKQKPYRRAFMESFGA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHCC
RCIASPSQETAFGRSVLADNPASTGSLGIAIGEAVELAAQRDDTKYALGSVLNHVLLHQT
CEECCCCHHHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VTGVEAMEQMAMADDYPDTVIACTGGGSNFAGIVFPFLGAALRGGPSVEAIAVEPLACPT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
LTRGQFAYDYGDSAQLTPLTKMHTLGSGFMPPGFHAGGLRYHGMAPQISHLKDLGYLDAR
CCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCHHHH
AYDQLDCFAAGLEFARAEGIIPAPEANHAVKAVIEEAQGCRERGESKAILFNLCGHGNFD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
MQAYTDYLAGNLSDLGGSQVEAAMALSGLPSVER
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SETVKYVLDEARIPRAWYNISADLPAPLAPVLHPATREPVSADDLAPLFPRGVIEQELT
CHHHHHHHHHHCCCHHHEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHC
TEREVEIPEPVRNAYRQWRPAPLYRARRLEKALDTPARIYYKYEGVSPAGSHKPNTALPQ
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHH
AFYNHAEGVRRITTETGAGQWGSSLALAGAMFDLDIEVFMVGVSYKQKPYRRAFMESFGA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHCC
RCIASPSQETAFGRSVLADNPASTGSLGIAIGEAVELAAQRDDTKYALGSVLNHVLLHQT
CEECCCCHHHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VTGVEAMEQMAMADDYPDTVIACTGGGSNFAGIVFPFLGAALRGGPSVEAIAVEPLACPT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
LTRGQFAYDYGDSAQLTPLTKMHTLGSGFMPPGFHAGGLRYHGMAPQISHLKDLGYLDAR
CCCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCHHHH
AYDQLDCFAAGLEFARAEGIIPAPEANHAVKAVIEEAQGCRERGESKAILFNLCGHGNFD
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
MQAYTDYLAGNLSDLGGSQVEAAMALSGLPSVER
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]