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The map label for this gene is gltB [H]

Identifier: 121998395

GI number: 121998395

Start: 1758082

End: 1759548

Strand: Reverse

Name: gltB [H]

Synonym: Hhal_1616

Alternate gene names: 121998395

Gene position: 1759548-1758082 (Counterclockwise)

Preceding gene: 121998396

Following gene: 121998392

Centisome position: 65.69

GC content: 62.58

Gene sequence:

>1467_bases
ATGGGTAAACAAACTGGATTCTTGGAACACGAGCGCCAAGAGTACGGCTATCAGCCGGCCTGTAAGCGCGTCTTCCACTA
CGGGGAGTTCCTGATCCCGCTCAGCCGGGAGCAAGTCGGCCAACAGGGGGCTCGCTGTATGGACTGCGGCATCCCCTTCT
GCCACAGCGGCTGTCCGGTGGACAACCTGATCCCGAACTGGAACGACATGGTCTACCGCGGTGAATGGGAGCGGGCCGTC
GAAGAGTTGCACGCCACCAACAACTTCCCTGAATTCACCGGCAGGGTCTGCCCGGCACCCTGTGAGGCCTCCTGTACCCT
GAACCTCGATGACAGTCCTGTATCCATCAAGACCATCGAATGCAGCATCGTCGATCGGGCGTGGGAAGCGGGGCTGATCC
GTCCCCGGGTGGCCCGCCGCCGGACGGGTAAGCGGATTGCCGTCGTTGGTTCCGGGCCGGCGGGCCTGGCCTGCGCCCAA
CAACTCGCGCGGGTTGGCCATAACGTTGATGTCTACGAGAAAGCCGACGCAATCGGCGGGCTCCTGCGATACGGCATCCC
CGATTTCAAGCTTGAGAAGAGGCACATCGACCGGCGTATCGCACAGATGCGCACCGAAGGGGTGCAGTTCCACACGTTGA
CCCATGTCGGGGTGGACATACCGATCGCCCAACTGCGTGATGAGTTTGATTCGGTGGTCCTCGCCGGTGGCAGTGAGCAG
CCTCGTGACCTGCCTGTGCCCGGTCGCGAACTCAACGGCGTCCACTTCGCCATGGAATTTTTGACCGCCAACAGCAAGCG
GGTCCAGGGCGTGTATGTGCCCGACGACGCGTTCATTGACGCCCAGGGCAAGCACGTGGTGGTCATCGGTGGCGGCGATA
CGGGCTCCGACTGTGTGGGGACCTCGTGTCGTCATGGGGCACGCTCTGTGACCCAGGTGGAAATCCTCAATAAGCCGCCG
GAAAAAGAGGATAAAGAGCTGACCTGGCCCTACTGGCCGGAGAAACTCAACACATCGACCTCCCACCAAGAGGGGTGCGA
GCGGATGTGGAACTTCCTGACCAAGGAGTTCGTCGGCGATGGCAGCGGCCATGTCCGCGCGATCCGTTACGCCAAGGTTG
AGTGGCACAAGGACCAGCAGGGCCGCTGGCAGATGAGCGAGATCCCCGGTAGCGAGGCCGAGATGCCGGCCGATCGGGTC
TTTCTGGCTATGGGGTTTCTCCACCCGGTCCGTGCGGGGATGATCGCTGAACTGGAGGAGAAGGGGGGGCTGGAACTCGA
TGACCGCGGCAATGTACGCGGCGATACTGAAGGAGCGGATGCCTACAAGACCAGTACGGATGGCGTCTTCGTCGCCGGAG
ACATGCGTCGGGGCCAGTCACTCGTGGTCTGGGCAATCCGCGAAGGCCGGCAGTGCGCTCATGCGGTCGACAAGTGGCTG
CAGGGCACGAGCGATCTGCCCCGCTGA

Upstream 100 bases:

>100_bases
TTCCGCCGCGCACTCGACGAGATCGAGGCGCAACGGGCACAGCCACCGTCGCCGACCCGGCCGGCGAAGTTTCATCAGGC
TCTCCAGGGGAGCGTTGATC

Downstream 100 bases:

>100_bases
TCGAAGATCGCTGGAAGAGGATACGCCCACCATGGATTTGCCTGGTTTCATCATCTTCAAGCTAGCGCTCGTGGCGCTGG
CCATGGGCTACGCAGTGCGA

Product: glutamate synthase NADH/NADPH small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKQTGFLEHERQEYGYQPACKRVFHYGEFLIPLSREQVGQQGARCMDCGIPFCHSGCPVDNLIPNWNDMVYRGEWERAV
EELHATNNFPEFTGRVCPAPCEASCTLNLDDSPVSIKTIECSIVDRAWEAGLIRPRVARRRTGKRIAVVGSGPAGLACAQ
QLARVGHNVDVYEKADAIGGLLRYGIPDFKLEKRHIDRRIAQMRTEGVQFHTLTHVGVDIPIAQLRDEFDSVVLAGGSEQ
PRDLPVPGRELNGVHFAMEFLTANSKRVQGVYVPDDAFIDAQGKHVVVIGGGDTGSDCVGTSCRHGARSVTQVEILNKPP
EKEDKELTWPYWPEKLNTSTSHQEGCERMWNFLTKEFVGDGSGHVRAIRYAKVEWHKDQQGRWQMSEIPGSEAEMPADRV
FLAMGFLHPVRAGMIAELEEKGGLELDDRGNVRGDTEGADAYKTSTDGVFVAGDMRRGQSLVVWAIREGRQCAHAVDKWL
QGTSDLPR

Sequences:

>Translated_488_residues
MGKQTGFLEHERQEYGYQPACKRVFHYGEFLIPLSREQVGQQGARCMDCGIPFCHSGCPVDNLIPNWNDMVYRGEWERAV
EELHATNNFPEFTGRVCPAPCEASCTLNLDDSPVSIKTIECSIVDRAWEAGLIRPRVARRRTGKRIAVVGSGPAGLACAQ
QLARVGHNVDVYEKADAIGGLLRYGIPDFKLEKRHIDRRIAQMRTEGVQFHTLTHVGVDIPIAQLRDEFDSVVLAGGSEQ
PRDLPVPGRELNGVHFAMEFLTANSKRVQGVYVPDDAFIDAQGKHVVVIGGGDTGSDCVGTSCRHGARSVTQVEILNKPP
EKEDKELTWPYWPEKLNTSTSHQEGCERMWNFLTKEFVGDGSGHVRAIRYAKVEWHKDQQGRWQMSEIPGSEAEMPADRV
FLAMGFLHPVRAGMIAELEEKGGLELDDRGNVRGDTEGADAYKTSTDGVFVAGDMRRGQSLVVWAIREGRQCAHAVDKWL
QGTSDLPR
>Mature_487_residues
GKQTGFLEHERQEYGYQPACKRVFHYGEFLIPLSREQVGQQGARCMDCGIPFCHSGCPVDNLIPNWNDMVYRGEWERAVE
ELHATNNFPEFTGRVCPAPCEASCTLNLDDSPVSIKTIECSIVDRAWEAGLIRPRVARRRTGKRIAVVGSGPAGLACAQQ
LARVGHNVDVYEKADAIGGLLRYGIPDFKLEKRHIDRRIAQMRTEGVQFHTLTHVGVDIPIAQLRDEFDSVVLAGGSEQP
RDLPVPGRELNGVHFAMEFLTANSKRVQGVYVPDDAFIDAQGKHVVVIGGGDTGSDCVGTSCRHGARSVTQVEILNKPPE
KEDKELTWPYWPEKLNTSTSHQEGCERMWNFLTKEFVGDGSGHVRAIRYAKVEWHKDQQGRWQMSEIPGSEAEMPADRVF
LAMGFLHPVRAGMIAELEEKGGLELDDRGNVRGDTEGADAYKTSTDGVFVAGDMRRGQSLVVWAIREGRQCAHAVDKWLQ
GTSDLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=475, Percent_Identity=23.7894736842105, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI111118983, Length=155, Percent_Identity=33.5483870967742, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI111118981, Length=119, Percent_Identity=38.655462184874, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789606, Length=479, Percent_Identity=36.9519832985386, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1788811, Length=463, Percent_Identity=36.7170626349892, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI87082180, Length=462, Percent_Identity=34.8484848484849, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1788468, Length=447, Percent_Identity=27.5167785234899, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=26.3279445727483, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=502, Percent_Identity=42.6294820717131, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984108, Length=467, Percent_Identity=24.4111349036403, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17543860, Length=168, Percent_Identity=34.5238095238095, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=498, Percent_Identity=46.1847389558233, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28574881, Length=498, Percent_Identity=48.995983935743, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=498, Percent_Identity=48.995983935743, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=498, Percent_Identity=48.995983935743, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=498, Percent_Identity=48.995983935743, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=471, Percent_Identity=25.0530785562633, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18858217, Length=471, Percent_Identity=25.0530785562633, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137178, Length=185, Percent_Identity=29.1891891891892, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54267; Mature: 54136

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQTGFLEHERQEYGYQPACKRVFHYGEFLIPLSREQVGQQGARCMDCGIPFCHSGCPV
CCCCCCCHHHHHHHCCCCHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCCCCCCCCCH
DNLIPNWNDMVYRGEWERAVEELHATNNFPEFTGRVCPAPCEASCTLNLDDSPVSIKTIE
HHCCCCCCCEEECCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEECCCCCCEEEEEE
CSIVDRAWEAGLIRPRVARRRTGKRIAVVGSGPAGLACAQQLARVGHNVDVYEKADAIGG
EEEEHHHHHCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LLRYGIPDFKLEKRHIDRRIAQMRTEGVQFHTLTHVGVDIPIAQLRDEFDSVVLAGGSEQ
HHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCEEEECCCCC
PRDLPVPGRELNGVHFAMEFLTANSKRVQGVYVPDDAFIDAQGKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCCEEEEEECCCCCCCCCC
TSCRHGARSVTQVEILNKPPEKEDKELTWPYWPEKLNTSTSHQEGCERMWNFLTKEFVGD
CCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCC
GSGHVRAIRYAKVEWHKDQQGRWQMSEIPGSEAEMPADRVFLAMGFLHPVRAGMIAELEE
CCCCEEEEEEEEEEECCCCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGGLELDDRGNVRGDTEGADAYKTSTDGVFVAGDMRRGQSLVVWAIREGRQCAHAVDKWL
CCCCEECCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHH
QGTSDLPR
CCCCCCCC
>Mature Secondary Structure 
GKQTGFLEHERQEYGYQPACKRVFHYGEFLIPLSREQVGQQGARCMDCGIPFCHSGCPV
CCCCCCHHHHHHHCCCCHHHHHHHHCCCEEEECCHHHHHHCCCEEECCCCCCCCCCCCH
DNLIPNWNDMVYRGEWERAVEELHATNNFPEFTGRVCPAPCEASCTLNLDDSPVSIKTIE
HHCCCCCCCEEECCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEECCCCCCEEEEEE
CSIVDRAWEAGLIRPRVARRRTGKRIAVVGSGPAGLACAQQLARVGHNVDVYEKADAIGG
EEEEHHHHHCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LLRYGIPDFKLEKRHIDRRIAQMRTEGVQFHTLTHVGVDIPIAQLRDEFDSVVLAGGSEQ
HHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCEEEECCCCC
PRDLPVPGRELNGVHFAMEFLTANSKRVQGVYVPDDAFIDAQGKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCCEEEEEECCCCCCCCCC
TSCRHGARSVTQVEILNKPPEKEDKELTWPYWPEKLNTSTSHQEGCERMWNFLTKEFVGD
CCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCC
GSGHVRAIRYAKVEWHKDQQGRWQMSEIPGSEAEMPADRVFLAMGFLHPVRAGMIAELEE
CCCCEEEEEEEEEEECCCCCCCEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGGLELDDRGNVRGDTEGADAYKTSTDGVFVAGDMRRGQSLVVWAIREGRQCAHAVDKWL
CCCCEECCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHH
QGTSDLPR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]