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The map label for this gene is tilS/hprT [H]

Identifier: 121998050

GI number: 121998050

Start: 1368324

End: 1368917

Strand: Reverse

Name: tilS/hprT [H]

Synonym: Hhal_1260

Alternate gene names: 121998050

Gene position: 1368917-1368324 (Counterclockwise)

Preceding gene: 121998051

Following gene: 121998049

Centisome position: 51.11

GC content: 65.66

Gene sequence:

>594_bases
ATGCCTTGGGACGATGCCCCGGATCTGCGCGGGGCCGAACTGATTCATGACCAGTGGGCCATCGATCAGGCCCTGGATCG
GATGGCCAGGGAGATCACGCAGGACCTTGGTGGCCGCAACCCGCTGATCCTGGGTGTCATGATCGGTGGCATGATCCCCG
CCGGGCGGTTGCTGCCGCGGCTCGATTTCCCGCTCGAGGTGGACTACGTCCACGCCACGCGGTATCGCAACGAGCTCAGC
GGCGAGGAGCTGGTCTGGCACGCCCTGCCTACGACGCCGATCGAAGGGCGCGACCTGCTGGTGGTCGATGACATCCTCGA
TGAGGGCAACACCCTGGCCGGGATCATCGACGCCTTCCGCAGCGCAGGTGCCCGTTCGGTGCGCACGGCCGTGCTGGTCG
ATAAGCGCCACGACCGCAAGTATCAGGGGCTGTGCGCCGATTACGTCGGCTTGGAAGTGGAGGATCGCTACGTCTTCGGC
GCCGGGATGGACTGTCGGGGCCTCGGCCGCAACGCGGACGGCATCTACGCCATCGATCAGCGCATCGAACAGGCGGCGAA
AACCCAGCAAGAACCGGATACGGAGGAGCGTTGA

Upstream 100 bases:

>100_bases
GCCTACCAGACAGCGGTCTGCGAATTGACCGAGGGCGTTCCCGGGTTCGGGGCGCCGGGGTGAAGACACCGAAACAGAGA
ACAGTACCAGGAGGGCTGCA

Downstream 100 bases:

>100_bases
TGAGTGTCGGCATTATCGGCGGGACGGGGCTGACGTCCCTGGAAGGGCTGGAAATTACCCGCCGCGAGGTGGTCCGGACC
CCGTTCGGCGAGCCTTCGGC

Product: phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MPWDDAPDLRGAELIHDQWAIDQALDRMAREITQDLGGRNPLILGVMIGGMIPAGRLLPRLDFPLEVDYVHATRYRNELS
GEELVWHALPTTPIEGRDLLVVDDILDEGNTLAGIIDAFRSAGARSVRTAVLVDKRHDRKYQGLCADYVGLEVEDRYVFG
AGMDCRGLGRNADGIYAIDQRIEQAAKTQQEPDTEER

Sequences:

>Translated_197_residues
MPWDDAPDLRGAELIHDQWAIDQALDRMAREITQDLGGRNPLILGVMIGGMIPAGRLLPRLDFPLEVDYVHATRYRNELS
GEELVWHALPTTPIEGRDLLVVDDILDEGNTLAGIIDAFRSAGARSVRTAVLVDKRHDRKYQGLCADYVGLEVEDRYVFG
AGMDCRGLGRNADGIYAIDQRIEQAAKTQQEPDTEER
>Mature_196_residues
PWDDAPDLRGAELIHDQWAIDQALDRMAREITQDLGGRNPLILGVMIGGMIPAGRLLPRLDFPLEVDYVHATRYRNELSG
EELVWHALPTTPIEGRDLLVVDDILDEGNTLAGIIDAFRSAGARSVRTAVLVDKRHDRKYQGLCADYVGLEVEDRYVFGA
GMDCRGLGRNADGIYAIDQRIEQAAKTQQEPDTEER

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=165, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17510817, Length=146, Percent_Identity=28.0821917808219, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 21997; Mature: 21866

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWDDAPDLRGAELIHDQWAIDQALDRMAREITQDLGGRNPLILGVMIGGMIPAGRLLPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHCCC
LDFPLEVDYVHATRYRNELSGEELVWHALPTTPIEGRDLLVVDDILDEGNTLAGIIDAFR
CCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHH
SAGARSVRTAVLVDKRHDRKYQGLCADYVGLEVEDRYVFGAGMDCRGLGRNADGIYAIDQ
HCCCHHHHHHHEEECCCCCHHHHHHHHHHCCEEECCEEEECCCCCCCCCCCCCCHHHHHH
RIEQAAKTQQEPDTEER
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PWDDAPDLRGAELIHDQWAIDQALDRMAREITQDLGGRNPLILGVMIGGMIPAGRLLPR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHCCC
LDFPLEVDYVHATRYRNELSGEELVWHALPTTPIEGRDLLVVDDILDEGNTLAGIIDAFR
CCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHH
SAGARSVRTAVLVDKRHDRKYQGLCADYVGLEVEDRYVFGAGMDCRGLGRNADGIYAIDQ
HCCCHHHHHHHEEECCCCCHHHHHHHHHHCCEEECCEEEECCCCCCCCCCCCCCHHHHHH
RIEQAAKTQQEPDTEER
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]