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The map label for this gene is recF [H]

Identifier: 121998015

GI number: 121998015

Start: 1331199

End: 1332248

Strand: Reverse

Name: recF [H]

Synonym: Hhal_1225

Alternate gene names: 121998015

Gene position: 1332248-1331199 (Counterclockwise)

Preceding gene: 121998016

Following gene: 121998014

Centisome position: 49.74

GC content: 68.76

Gene sequence:

>1050_bases
ATGGAACGCCTGGAGGTCCACGGCTTCCGGAACCTGGCCGATACCCAACTGCGGCCCCATCCCCACCTCAACGTGGTGAC
CGGGCCGAATGGCGCGGGAAAGACCAGTCTGCTCGAGGCGATCTACTTCCTCTCTCGGGTCCGTTCATTCCGCACCCGGC
AGAACGACCGTCTCATCGGCTGGGGGGCCGAGGAGGCCCGCGTGGTCGCCGTGCGCGGCCACGACCGCCTCGGTGCCGCC
CGTACCCCCGGCCACACGCGACTGCGGCTGAACGGTGCCGATGCCCATACCCGCTCGGCCCTGGCCGCTCGGCTTCCGGT
TCAGCTGATGAACACCGAGCACCAGCGGCTGCTCCTTGACGGTCCCCGGGTGCGCCGCCAGTTCCTCGACTGGGGGACGT
TCCACCTGGAACCGGACTACCGCGAACTGGCCCAGCGTTATCAGCACGCCCTGCGCCAGCGCAACGCCGCCCTTCGGGTC
GGCGACCGGCGCAGCGAACAGGCCTGGACCCCGGTCCTCATCCGTTGTGCCGCCGCCGTGGACGTTGCCCGCCAGCGGTT
CATTGAGGCCCTGCGTCCCATCTGGAGCGAATTCGCCCGGCAGTGGCTGGGCCTGGAGAGCCTGGAACTGCGTTACTACC
GGGGGGCTGCCGCCGAATTGCCGTGGGAACGGGTCCTCGATGAGCAGCTCGAGCGCGACCGCACTGCCGGGTTCACCCAC
CGCGGCCCCCACCGGGCCGATCTGATCCTCAGCCGGGATCGCATCCCCGCTGCCGATGCCCTCTCGCGGGGTCAGCAGAA
GCTACTGGTGGTGGCCCTGCTGATCGCCGAGGTGAAGCTCTGGTCCCGCCAGGGGCTGACCCCGGTGCTGCTGATCGACG
ATCTCCCCGCCGAGCTGGATCCGGCGCACCTGCACACCGTGCTGGATACGGTGACCGGTGATCCCACCCAGGTCTTTCTG
ACCGCTATCGACGGCGCTGCGCTGCCGACCGATCTGCCCCCGGGACAGTGGTATCAGGTATGCGAAGGGAGGGTGTCAGC
GATGGTATAA

Upstream 100 bases:

>100_bases
TCCAAGTGACCCTGACCGACGCCAGCAGCAGCGGCCTGATCCGTGCCGAGGGGTCCGACAATGCGCGCTACGTCGTTATG
CCGATGCGCCTCTGAACCGA

Downstream 100 bases:

>100_bases
TGGCCTTTTACGACCGGCACTTCGGTCCAGACGTACCCTTGTAGAGGCTGAATCATCGATGACGACGGAGATTACGCCGC
AGTACGACTCGGAAAGCATT

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MERLEVHGFRNLADTQLRPHPHLNVVTGPNGAGKTSLLEAIYFLSRVRSFRTRQNDRLIGWGAEEARVVAVRGHDRLGAA
RTPGHTRLRLNGADAHTRSALAARLPVQLMNTEHQRLLLDGPRVRRQFLDWGTFHLEPDYRELAQRYQHALRQRNAALRV
GDRRSEQAWTPVLIRCAAAVDVARQRFIEALRPIWSEFARQWLGLESLELRYYRGAAAELPWERVLDEQLERDRTAGFTH
RGPHRADLILSRDRIPAADALSRGQQKLLVVALLIAEVKLWSRQGLTPVLLIDDLPAELDPAHLHTVLDTVTGDPTQVFL
TAIDGAALPTDLPPGQWYQVCEGRVSAMV

Sequences:

>Translated_349_residues
MERLEVHGFRNLADTQLRPHPHLNVVTGPNGAGKTSLLEAIYFLSRVRSFRTRQNDRLIGWGAEEARVVAVRGHDRLGAA
RTPGHTRLRLNGADAHTRSALAARLPVQLMNTEHQRLLLDGPRVRRQFLDWGTFHLEPDYRELAQRYQHALRQRNAALRV
GDRRSEQAWTPVLIRCAAAVDVARQRFIEALRPIWSEFARQWLGLESLELRYYRGAAAELPWERVLDEQLERDRTAGFTH
RGPHRADLILSRDRIPAADALSRGQQKLLVVALLIAEVKLWSRQGLTPVLLIDDLPAELDPAHLHTVLDTVTGDPTQVFL
TAIDGAALPTDLPPGQWYQVCEGRVSAMV
>Mature_349_residues
MERLEVHGFRNLADTQLRPHPHLNVVTGPNGAGKTSLLEAIYFLSRVRSFRTRQNDRLIGWGAEEARVVAVRGHDRLGAA
RTPGHTRLRLNGADAHTRSALAARLPVQLMNTEHQRLLLDGPRVRRQFLDWGTFHLEPDYRELAQRYQHALRQRNAALRV
GDRRSEQAWTPVLIRCAAAVDVARQRFIEALRPIWSEFARQWLGLESLELRYYRGAAAELPWERVLDEQLERDRTAGFTH
RGPHRADLILSRDRIPAADALSRGQQKLLVVALLIAEVKLWSRQGLTPVLLIDDLPAELDPAHLHTVLDTVTGDPTQVFL
TAIDGAALPTDLPPGQWYQVCEGRVSAMV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=355, Percent_Identity=34.9295774647887, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 39491; Mature: 39491

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLEVHGFRNLADTQLRPHPHLNVVTGPNGAGKTSLLEAIYFLSRVRSFRTRQNDRLIG
CCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
WGAEEARVVAVRGHDRLGAARTPGHTRLRLNGADAHTRSALAARLPVQLMNTEHQRLLLD
ECCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHCCCCCEEEEC
GPRVRRQFLDWGTFHLEPDYRELAQRYQHALRQRNAALRVGDRRSEQAWTPVLIRCAAAV
CHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
DVARQRFIEALRPIWSEFARQWLGLESLELRYYRGAAAELPWERVLDEQLERDRTAGFTH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCC
RGPHRADLILSRDRIPAADALSRGQQKLLVVALLIAEVKLWSRQGLTPVLLIDDLPAELD
CCCCCHHEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PAHLHTVLDTVTGDPTQVFLTAIDGAALPTDLPPGQWYQVCEGRVSAMV
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCHHHHCC
>Mature Secondary Structure
MERLEVHGFRNLADTQLRPHPHLNVVTGPNGAGKTSLLEAIYFLSRVRSFRTRQNDRLIG
CCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
WGAEEARVVAVRGHDRLGAARTPGHTRLRLNGADAHTRSALAARLPVQLMNTEHQRLLLD
ECCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHCCCCCEEEEC
GPRVRRQFLDWGTFHLEPDYRELAQRYQHALRQRNAALRVGDRRSEQAWTPVLIRCAAAV
CHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHH
DVARQRFIEALRPIWSEFARQWLGLESLELRYYRGAAAELPWERVLDEQLERDRTAGFTH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCC
RGPHRADLILSRDRIPAADALSRGQQKLLVVALLIAEVKLWSRQGLTPVLLIDDLPAELD
CCCCCHHEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PAHLHTVLDTVTGDPTQVFLTAIDGAALPTDLPPGQWYQVCEGRVSAMV
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA